Dynamic oligomer, primarily tetramer by size-exclusion chromatography.

All shifts referenced to water frequency, using DSS standard for carbon and proton shifts, and 15N shifts referenced to anhydrous liquid ammonia

Proceedings of the National Academy of Sciences of the United States of America
A soluble α-synuclein construct forms a dynamic tetramer.

Rosetta strain is optimized for expression of human proteins. Protein expressed as a GST fusion.

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0.5 mM (monomer) U-15N,13C labeled aSyn in 100 mM HEPES, pH 7.4, 150 mM NaCl, 10% glycerol, 0.1% n-octyl-beta-glucopyranoside (BOG) (BOG conc much less than CMC)

0.5 mM (monomer) in 100 mM HEPES, pH 7.4, 150 mM NaCl, 10% glycerol, 0.1% n-octyl-beta-glucopyranoside (BOG) (BOG conc much less than CMC)