Solution NMR structure of the parkin Ubl domain in complex with the endophilin-A1 SH3 domain

SH3 domains from a subset of BAR proteins define a Ubl-binding domain and implicate parkin in synaptic ubiquitination.

Residues 1-5 represent a non-native tag generated after 3C protease cleavage of N-terminal GST

Residues 1-9 represent a non-native tag generated after thrombin protease cleavage of N-terminal GST

Crystal Structure Of Parkin E3 Ubiquitin Ligase (linker Deletion; Delta 86-130)

E3 ubiquitin-protein ligase parkin [Rattus norvegicus]

RecName: Full=E3 ubiquitin-protein ligase parkin

Crystal Structure Of The Rat Endophilin-A1 Sh3 Domain

SH3-domain GRB2-like 2 protein [Oryctolagus cuniculus]

PREDICTED: endophilin-A1-like [Ailuropoda melanoleuca]

PREDICTED: endophilin-A1 [Otolemur garnettii]

RecName: Full=Endophilin-A1; AltName: Full=Endophilin-1; AltName: Full=SH3 domain protein 2A; AltName: Full=SH3 domain-containi

TPA: SH3-domain GRB2-like 2 [Bos taurus]

10 mM HEPES
50 mM NaCl
1 mM DTT
0.1 mM EDTA

4.5% polyacrylamide
10 mM HEPES
50 mM NaCl
1 mM DTT
0.1 mM EDTA

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Brunger, Adams, Clore, Gros, Nilges and Read