Backbone 1H, 13C, and 15N Chemical Shift Assignments for Oryctin

Isolation, cDNA cloning, and structure-based functional characterization of oryctin, a hemolymph protein from the coconut rhinoceros beetle, Oryctes rhinoceros, as a novel serine protease inhibitor.

hemolymph of the coconut rhinoceros beetle, Oryctes rhinoceros

DGSA-distance geometry simulated annealing

13C,15N-labeled oryctin was dissolved into 50 mM sodium phosphate buffer (pH 6.8),
100 mM NaCl, 0.02% NaN3 in 90% H2O/10% D2O (v/v) to a final concentration of 2 mM.

NPPSFA, National Project on Protein Structural and Functional Analyses