D-G3P-bound WT yeast Triosephosphate Isomerase (TIM)

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(2R)-2,3-dihydroxypropyl dihydrogen phosphate

[(2R)-2,3-dihydroxypropyl] dihydrogen phosphate

Triosephosphate isomerase: 15N and 13C chemical shift assignments and conformational change upon ligand binding by magic-angle spinning solid-state NMR spectroscopy.

Yeast Triosephosphate Isomerase (Mutant)

Triosephosphate Isomerase In Complex With Dhap

Structure Of Yeast Triosephosphate Isomerase At 1.9- Angstroms Resolution

Crystallographic Analysis Of The Complex Between Triosephosphate Isomerase And 2-Phosphoglycolate At 2.5- Angstroms Resolution.

Electrophilic Catalysis In Triosephosphase Isomerase: The Role Of Histidine-95

Structure Of C126s Mutant Of Saccharomyces Cerevisiae Triosephosphate Isomerase

Structure Of The Triosephosphate Isomerase- Phosphoglycolohydroxamate Complex: An Analogue Of The Intermediate On The Reaction

K7_Tpi1p [Saccharomyces cerevisiae Kyokai no. 7]

triose phosphate isomerase [Saccharomyces cerevisiae]

Tpi1p [Saccharomyces cerevisiae YJM1078]

triose-phosphate isomerase TPI1 [Saccharomyces cerevisiae S288c]

RecName: Full=Triosephosphate isomerase; Short=TIM; AltName: Full=Triose-phosphate isomerase

TPA: triose-phosphate isomerase TPI1 [Saccharomyces cerevisiae S288c]

50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 6.8 at 4 C

Ligand was added in a 5:1 ratio to enzyme. Ligand saturation was monitored by
measured inhibition of the enzyme activity. The ligand and the precipitant were
dissolved in 10 mM Tris-HCl, pH 6.8 at 4 C to minimize the ionic strength.
Addition of the magnesium salt of the ligand did not change the solution pH.
After an hour of incubation at 4 C, 40% (w/v) PEG4K was added to reach the
final concentration of 15%. After an overnight incubation at 4 C, crystals were
visible. A typical 4 mm SSNMR Varian rotor contained 20 mg of hydrated,
microcrystalline protein, approximately 35 μl of sample.

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax