NMR Solution structure of a diflavin flavoprotein A3 from Nostoc sp. PCC 7120, Northeast Structural Genomics Consortium Target NsR431C

Northeast Structural Genomics Consortium

Mediates electron transfer from NADH to oxygen, reducing it to water. This modular protein has 3 redox cofactors, in other organisms the same activity requires 2 or 3 proteins (By similarity)

Nmr Solution Structure Of A Diflavin Flavoprotein A3 From Nostoc Sp. Pcc 7120, Northeast Structural Genomics Consortium Target

Crystal Structure Of A Diflavin Flavoprotein A3 (all3895) From Nostoc Sp., Northeast Structural Genomics Consortium Target Nsr4

diflavin flavoprotein A [Nostoc sp. PCC 7120]

RecName: Full=Putative diflavin flavoprotein A 3

Data acquisition on B600 was performed using 35ul of 1.18mM sample and a 1.7mm microcryoprobe.

1.18mM NsR431C, 10mM DTT, 50uM DSS, 0.02% NaN3,200mM NaCl, 5mM CaCl2, 20mM MES, protease inhibitors added.
Zimmerman, Moseley, Kulikowski and Montelione
Brunger, Adams, Clore, Gros, Nilges and Read