1H, 13C, and 15N Chemical Shift Assignments for Myosin Phosphatase Trageting Subunit 1 (residues 1-98)

Understanding the assembly of the myosin phosphatase holoenzyme

MYPT1 is a 110 kDa protein. In this study, we used a shorter construct
comprising the first 98 residues of the protein. This construct, refered
as MYPT1 1-98, is part of the PP1-binding domain of MYPT1.

The first 2 residues (Gly1 and His2) are part of the TEV protease cleavage site used during production of the protein.

Regulatory (targeting) subunit of protein phosphatase 1 in the myosin phosphatase holoenzyme.

Complex Between Protein Ser/thr Phosphatase-1 (delta) And The Myosin Phosphatase Targeting Subunit 1 (mypt1)

133kDa myosin-binding subunit of smooth muscle myosin phosphatase (M133) [Gallus gallus]

130 kDa myosin-binding subunit of smooth muscle myosin phophatase (M130) [Gallus gallus]

hypothetical protein RCJMB04_22k13, partial [Gallus gallus]

hypothetical protein AAES_00677 [Amazona aestiva]

protein phosphatase 1, regulatory subunit 12A [Alligator mississippiensis]

protein phosphatase 1 regulatory subunit 12A [Gallus gallus]

PREDICTED: protein phosphatase 1 regulatory subunit 12A isoform X1 [Taeniopygia guttata]

PREDICTED: protein phosphatase 1 regulatory subunit 12A isoform X1 [Ficedula albicollis]

PREDICTED: protein phosphatase 1 regulatory subunit 12A isoform X2 [Ficedula albicollis]

PREDICTED: protein phosphatase 1 regulatory subunit 12A isoform X3 [Ficedula albicollis]

RecName: Full=Protein phosphatase 1 regulatory subunit 12A; AltName: Full=130 kDa myosin-binding subunit of smooth muscle myosi

MYPT1 was expressed as a histag fusion protein with a cleavage site for TEV protease.

Prof. Kurt Wuthrich
Institute of Molecular Biology
and Biophysics
ETH Honggerberg
CH-8093 Zurich

Bruker BioSpin GmbH
Silberstreifen 4
76287 Rheinstetten
Germany