NMR structures of GA95 and GB95, two designed proteins with 95% sequence identity but different folds and functions

Protein science : a publication of the Protein Society
De novo structure generation using chemical shifts for proteins with high-sequence identity but different folds.

with a z-axis gradient triple-resonance cryoprobe

0.15~0.3 mM Gb95 protein + pH7.2 100 mM potassium phosphate + 10% D2O

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Brunger, Adams, Clore, Gros, Nilges and Read