NMR solution Structure of Membrane associated protein from Bacillus cereus: Northeast Structural Genomics Consortium Target: BcR97A

Internal DSS was used to reference the proton spectrum

The sequence used to solve the structure is a construct (21-117aa) of the full length BcR97. The construct has been designed eliminating the first 20 residues based on the disorder-prediction algorithms. The residues 21-117 have been numbered as 1-97 in the current entry. The 8 residues at the C-terminal, LEHHHHHH correspond to the purification tag.

Nmr Solution Structure Of Membrane Associated Protein From Bacillus Cereus: Northeast Structural Genomics Consortium Target: Bc

putative lipoprotein [Bacillus thuringiensis serovar tolworthi]

Uncharacterized protein conserved in bacteria [Streptococcus pneumoniae]

hypothetical Membrane Associated Protein [Bacillus cereus ATCC 14579]

hypothetical protein BMB171_C4548 [Bacillus thuringiensis BMB171]

hypothetical protein CT43_CH4991 [Bacillus thuringiensis serovar chinensis CT-43]

hypothetical protein BTB_c51580 [Bacillus thuringiensis Bt407]

hypothetical protein H175_ch5073 [Bacillus thuringiensis serovar thuringiensis str. IS5056]

hypothetical protein BC4932 [Bacillus cereus ATCC 14579]

MULTISPECIES: membrane protein [Bacillus cereus group]

Northeast Structural Genomics Consortium

GFT (4,3)D data was acquired for backbone assignments.

1.25mM BcR97A, 10mM DTT, 50uM DSS, 10% D2O, 0.02% NaN3, 5mM CaCl2, 100mM NaCl, 20mM MES, protease inhibitors added.

0.77mM BcR97A, 10mM DTT, 50uM DSS, 10% D2O, 0.02% NaN3, 5mM CaCl2, 100mM NaCl, 20mM MES, protease inhibitors added.

Zimmerman, Moseley, Kulikowski and Montelione

Brunger, Adams, Clore, Gros, Nilges and Read