Dynamics and Calcium-Dependent Actin Bundling by the two Carboxy Terminal Domains of Villin

The isolated sixth gelsolin repeat and headpiece domain of villin bundle F-actin in the presence of calcium and are linked by a 40-residue unstructured sequence.

Sixth Gelsolin-like Domain Of Villin In 5 Mm Cacl2

The construct was cloned from full-length chicken villin cDNA (a gift of Dr. Paul Matsudaira,
Whitehead Institute/M.I.T.) into the pET-24a vector (Novagen) using standard protocols.

D6-HP is a C-terminal modular fragment of chicken villin containing the core domain 6 and headpiece.

Department of Physiology and Biophysics, Boston University School of Medicine

WR Wiley Environmental Molecular Sciences Laboratory, Hight Field NMR Facility

NOE value here is a ratio of NOE signal with presat over NOE w/out presat

http://xbeams.chem.yale.edu/~loria/
patrick.loria@yale.edu