NMR structure of the murine DLC2 (deleted in liver cancer -2) SAM (sterile alpha motif) domain

The NMR structure of the murine DLC2 SAM domain reveals a variant fold that is similar to a four-helix bundle.

The expressed protein contains the following additional amino acids not present
in the deposited structure. At the amino terminus - an terminal affinity tag, a
thrombin cleavage sequence (GSSHHHHHHSSGLVPRGSM) and several disordered amino
acids (LVTKIQQ). At the carboxy terminus, several disorderd amino acids
(MRLDVNFQRKKGDDSDEED).

StAR-related lipid transfer (START) domain containing 13 [Mus musculus]

StAR-related lipid transfer domain containing 13 [Rattus norvegicus]

serologically defined colon cancer antigen 13, isoform CRA_a, partial [Mus musculus]

serologically defined colon cancer antigen 13, isoform CRA_b, partial [Mus musculus]

stAR-related lipid transfer protein 13 [Rattus norvegicus]

stAR-related lipid transfer protein 13 isoform 1 [Mus musculus]

stAR-related lipid transfer protein 13 isoform 2 [Mus musculus]

PREDICTED: stAR-related lipid transfer protein 13 isoform X1 [Mesocricetus auratus]

PREDICTED: stAR-related lipid transfer protein 13 isoform X2 [Mesocricetus auratus]

RecName: Full=StAR-related lipid transfer protein 13; AltName: Full=START domain-containing protein 13; Short=StARD13

A DNA fragment from IMAGE cDNA 5320966 was amplified by PCR and inserted into NdeI and BamHI restriction sites of pET15b

The structure was determined using a combination of NOE restraints, amide
residual dipolar couplings and phi/psi/chi dihedral angle restraints predicted
by the PREDITOR software package.

0.9 mM protein concentration in a buffer comprised of 20 mM sodium phosphate, pH 7.8, 0.15 M sodium chloride, 0.05 % sodium azide

Using CYANA 2.1, 25 structures calculated from an initial ensemble of 250
structures. Further refinement of the 25 structures in water was performed
using XPLOR NIH 2.16.0. A total of 10 lowest structures were selected as the
final deposited ensemble.

Schwieters, Kuszewski, Tjandra and Clore