data_52630 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52630 _Entry.Title ; p14ARF forms meso-scale assemblies upon phase separation with NPM1 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2024-10-04 _Entry.Accession_date 2024-10-04 _Entry.Last_release_date 2024-10-04 _Entry.Original_release_date 2024-10-04 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Eric Gibbs . . . . 52630 2 Qi Miao . . . . 52630 3 Mylene Ferrolino . . . . 52630 4 Richa Bajpai . . . . 52630 5 Aila Hassan . . . . 52630 6 Aaron Phillips . . . . 52630 7 Aaron Pitre . . . . 52630 8 Rainer Kummerle . . . . 52630 9 Shondra Miller . . . . 52630 10 Gergely Nagy . . . . 52630 11 Wellington Leite . . . . 52630 12 William Heller . . . . 52630 13 Chris Stanley . . . . 52630 14 Barbara Perrone . . . . 52630 15 Richard Kriwacki . . . . 52630 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 52630 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 30 52630 '1H chemical shifts' 30 52630 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2025-01-09 . original BMRB . 52630 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 52628 'phase separated p14ARF-NPM1 meso-scale assembly, ARF solid assignment' 52630 BMRB 52629 'phase separated p14ARF-NPM1-IDR condensate, ARF solution assignment' 52630 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52630 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 39528457 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; p14ARF forms meso-scale assemblies upon phase separation with NPM1 ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Nat. Commun.' _Citation.Journal_name_full 'Nature Communications' _Citation.Journal_volume 15 _Citation.Journal_issue 1 _Citation.Journal_ASTM . _Citation.Journal_ISSN 2041-1723 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 9531 _Citation.Page_last 9531 _Citation.Year 2024 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Eric Gibbs . . . . 52630 1 2 Qi Miao . . . . 52630 1 3 Mylene Ferrolino . . . . 52630 1 4 Richa Bajpai . . . . 52630 1 5 Aila Hassan . . . . 52630 1 6 Aaron Phillips . . . . 52630 1 7 Aaron Pitre . . . . 52630 1 8 Rainer Kummerle . . . . 52630 1 9 Shondra Miller . . . . 52630 1 10 Gergely Nagy . . . . 52630 1 11 Wellington Leite . . . . 52630 1 12 William Heller . . . . 52630 1 13 Chris Stanley . . . . 52630 1 14 Barbara Perrone . . . . 52630 1 15 Richard Kriwacki . . . . 52630 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52630 _Assembly.ID 1 _Assembly.Name 'phase separated p14ARF-NPM1 condensate' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 NPM1 1 $entity_1 . . yes native no no . . . 52630 1 2 p14ARF 2 $entity_2 . . yes native no no . . . 52630 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52630 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GSMEDSMDMDMSPLRPQNYL FGCELKADKDYHFKVDNDEN EHQLSLRTVSLGAGAKDELH IVEAEAMNYEGSPIKVTLAT LKMSVQPTVSLGGFEITPPV VLRLKCGSGPVHISGQHLVA VEEDAESEDEEEEDVKLLSI SGKRSAPGGGSKVPQKKVKL AADEDDDDDDEEDDDEDDDD DDFDDEEAEEKAPVKKSIRD TPAKNAQKSNQNGKDSKPSS TPRSKGQESFKKQEKTPKTP KGPSSVEDIKAKMQASIEKG GSLPKVEAKFINYVKNCFRM TDQEAIQDLWQWRKSL ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq ; MEDSMDMDMSPLRPQNYLFG CELKADKDYHFKVDNDENEH QLSLRTVSLGAGAKDELHIV EAEAMNYEGSPIKVTLATLK MSVQPTVSLGGFEITPPVVL RLKCGSGPVHISGQHLVAVE EDAESEDEEEEDVKLLSISG KRSAPGGGSKVPQKKVKLAA DEDDDDDDEEDDDEDDDDDD FDDEEAEEKAPVKKSIRDTP AKNAQKSNQNGKDSKPSSTP RSKGQESFKKQEKTPKTPKG PSSVEDIKAKMQASIEKGGS LPKVEAKFINYVKNCFRMTD QEAIQDLWQWRKSL ; _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 296 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 -1 GLY . 52630 1 2 0 SER . 52630 1 3 1 MET . 52630 1 4 2 GLU . 52630 1 5 3 ASP . 52630 1 6 4 SER . 52630 1 7 5 MET . 52630 1 8 6 ASP . 52630 1 9 7 MET . 52630 1 10 8 ASP . 52630 1 11 9 MET . 52630 1 12 10 SER . 52630 1 13 11 PRO . 52630 1 14 12 LEU . 52630 1 15 13 ARG . 52630 1 16 14 PRO . 52630 1 17 15 GLN . 52630 1 18 16 ASN . 52630 1 19 17 TYR . 52630 1 20 18 LEU . 52630 1 21 19 PHE . 52630 1 22 20 GLY . 52630 1 23 21 CYS . 52630 1 24 22 GLU . 52630 1 25 23 LEU . 52630 1 26 24 LYS . 52630 1 27 25 ALA . 52630 1 28 26 ASP . 52630 1 29 27 LYS . 52630 1 30 28 ASP . 52630 1 31 29 TYR . 52630 1 32 30 HIS . 52630 1 33 31 PHE . 52630 1 34 32 LYS . 52630 1 35 33 VAL . 52630 1 36 34 ASP . 52630 1 37 35 ASN . 52630 1 38 36 ASP . 52630 1 39 37 GLU . 52630 1 40 38 ASN . 52630 1 41 39 GLU . 52630 1 42 40 HIS . 52630 1 43 41 GLN . 52630 1 44 42 LEU . 52630 1 45 43 SER . 52630 1 46 44 LEU . 52630 1 47 45 ARG . 52630 1 48 46 THR . 52630 1 49 47 VAL . 52630 1 50 48 SER . 52630 1 51 49 LEU . 52630 1 52 50 GLY . 52630 1 53 51 ALA . 52630 1 54 52 GLY . 52630 1 55 53 ALA . 52630 1 56 54 LYS . 52630 1 57 55 ASP . 52630 1 58 56 GLU . 52630 1 59 57 LEU . 52630 1 60 58 HIS . 52630 1 61 59 ILE . 52630 1 62 60 VAL . 52630 1 63 61 GLU . 52630 1 64 62 ALA . 52630 1 65 63 GLU . 52630 1 66 64 ALA . 52630 1 67 65 MET . 52630 1 68 66 ASN . 52630 1 69 67 TYR . 52630 1 70 68 GLU . 52630 1 71 69 GLY . 52630 1 72 70 SER . 52630 1 73 71 PRO . 52630 1 74 72 ILE . 52630 1 75 73 LYS . 52630 1 76 74 VAL . 52630 1 77 75 THR . 52630 1 78 76 LEU . 52630 1 79 77 ALA . 52630 1 80 78 THR . 52630 1 81 79 LEU . 52630 1 82 80 LYS . 52630 1 83 81 MET . 52630 1 84 82 SER . 52630 1 85 83 VAL . 52630 1 86 84 GLN . 52630 1 87 85 PRO . 52630 1 88 86 THR . 52630 1 89 87 VAL . 52630 1 90 88 SER . 52630 1 91 89 LEU . 52630 1 92 90 GLY . 52630 1 93 91 GLY . 52630 1 94 92 PHE . 52630 1 95 93 GLU . 52630 1 96 94 ILE . 52630 1 97 95 THR . 52630 1 98 96 PRO . 52630 1 99 97 PRO . 52630 1 100 98 VAL . 52630 1 101 99 VAL . 52630 1 102 100 LEU . 52630 1 103 101 ARG . 52630 1 104 102 LEU . 52630 1 105 103 LYS . 52630 1 106 104 CYS . 52630 1 107 105 GLY . 52630 1 108 106 SER . 52630 1 109 107 GLY . 52630 1 110 108 PRO . 52630 1 111 109 VAL . 52630 1 112 110 HIS . 52630 1 113 111 ILE . 52630 1 114 112 SER . 52630 1 115 113 GLY . 52630 1 116 114 GLN . 52630 1 117 115 HIS . 52630 1 118 116 LEU . 52630 1 119 117 VAL . 52630 1 120 118 ALA . 52630 1 121 119 VAL . 52630 1 122 120 GLU . 52630 1 123 121 GLU . 52630 1 124 122 ASP . 52630 1 125 123 ALA . 52630 1 126 124 GLU . 52630 1 127 125 SER . 52630 1 128 126 GLU . 52630 1 129 127 ASP . 52630 1 130 128 GLU . 52630 1 131 129 GLU . 52630 1 132 130 GLU . 52630 1 133 131 GLU . 52630 1 134 132 ASP . 52630 1 135 133 VAL . 52630 1 136 134 LYS . 52630 1 137 135 LEU . 52630 1 138 136 LEU . 52630 1 139 137 SER . 52630 1 140 138 ILE . 52630 1 141 139 SER . 52630 1 142 140 GLY . 52630 1 143 141 LYS . 52630 1 144 142 ARG . 52630 1 145 143 SER . 52630 1 146 144 ALA . 52630 1 147 145 PRO . 52630 1 148 146 GLY . 52630 1 149 147 GLY . 52630 1 150 148 GLY . 52630 1 151 149 SER . 52630 1 152 150 LYS . 52630 1 153 151 VAL . 52630 1 154 152 PRO . 52630 1 155 153 GLN . 52630 1 156 154 LYS . 52630 1 157 155 LYS . 52630 1 158 156 VAL . 52630 1 159 157 LYS . 52630 1 160 158 LEU . 52630 1 161 159 ALA . 52630 1 162 160 ALA . 52630 1 163 161 ASP . 52630 1 164 162 GLU . 52630 1 165 163 ASP . 52630 1 166 164 ASP . 52630 1 167 165 ASP . 52630 1 168 166 ASP . 52630 1 169 167 ASP . 52630 1 170 168 ASP . 52630 1 171 169 GLU . 52630 1 172 170 GLU . 52630 1 173 171 ASP . 52630 1 174 172 ASP . 52630 1 175 173 ASP . 52630 1 176 174 GLU . 52630 1 177 175 ASP . 52630 1 178 176 ASP . 52630 1 179 177 ASP . 52630 1 180 178 ASP . 52630 1 181 179 ASP . 52630 1 182 180 ASP . 52630 1 183 181 PHE . 52630 1 184 182 ASP . 52630 1 185 183 ASP . 52630 1 186 184 GLU . 52630 1 187 185 GLU . 52630 1 188 186 ALA . 52630 1 189 187 GLU . 52630 1 190 188 GLU . 52630 1 191 189 LYS . 52630 1 192 190 ALA . 52630 1 193 191 PRO . 52630 1 194 192 VAL . 52630 1 195 193 LYS . 52630 1 196 194 LYS . 52630 1 197 195 SER . 52630 1 198 196 ILE . 52630 1 199 197 ARG . 52630 1 200 198 ASP . 52630 1 201 199 THR . 52630 1 202 200 PRO . 52630 1 203 201 ALA . 52630 1 204 202 LYS . 52630 1 205 203 ASN . 52630 1 206 204 ALA . 52630 1 207 205 GLN . 52630 1 208 206 LYS . 52630 1 209 207 SER . 52630 1 210 208 ASN . 52630 1 211 209 GLN . 52630 1 212 210 ASN . 52630 1 213 211 GLY . 52630 1 214 212 LYS . 52630 1 215 213 ASP . 52630 1 216 214 SER . 52630 1 217 215 LYS . 52630 1 218 216 PRO . 52630 1 219 217 SER . 52630 1 220 218 SER . 52630 1 221 219 THR . 52630 1 222 220 PRO . 52630 1 223 221 ARG . 52630 1 224 222 SER . 52630 1 225 223 LYS . 52630 1 226 224 GLY . 52630 1 227 225 GLN . 52630 1 228 226 GLU . 52630 1 229 227 SER . 52630 1 230 228 PHE . 52630 1 231 229 LYS . 52630 1 232 230 LYS . 52630 1 233 231 GLN . 52630 1 234 232 GLU . 52630 1 235 233 LYS . 52630 1 236 234 THR . 52630 1 237 235 PRO . 52630 1 238 236 LYS . 52630 1 239 237 THR . 52630 1 240 238 PRO . 52630 1 241 239 LYS . 52630 1 242 240 GLY . 52630 1 243 241 PRO . 52630 1 244 242 SER . 52630 1 245 243 SER . 52630 1 246 244 VAL . 52630 1 247 245 GLU . 52630 1 248 246 ASP . 52630 1 249 247 ILE . 52630 1 250 248 LYS . 52630 1 251 249 ALA . 52630 1 252 250 LYS . 52630 1 253 251 MET . 52630 1 254 252 GLN . 52630 1 255 253 ALA . 52630 1 256 254 SER . 52630 1 257 255 ILE . 52630 1 258 256 GLU . 52630 1 259 257 LYS . 52630 1 260 258 GLY . 52630 1 261 259 GLY . 52630 1 262 260 SER . 52630 1 263 261 LEU . 52630 1 264 262 PRO . 52630 1 265 263 LYS . 52630 1 266 264 VAL . 52630 1 267 265 GLU . 52630 1 268 266 ALA . 52630 1 269 267 LYS . 52630 1 270 268 PHE . 52630 1 271 269 ILE . 52630 1 272 270 ASN . 52630 1 273 271 TYR . 52630 1 274 272 VAL . 52630 1 275 273 LYS . 52630 1 276 274 ASN . 52630 1 277 275 CYS . 52630 1 278 276 PHE . 52630 1 279 277 ARG . 52630 1 280 278 MET . 52630 1 281 279 THR . 52630 1 282 280 ASP . 52630 1 283 281 GLN . 52630 1 284 282 GLU . 52630 1 285 283 ALA . 52630 1 286 284 ILE . 52630 1 287 285 GLN . 52630 1 288 286 ASP . 52630 1 289 287 LEU . 52630 1 290 288 TRP . 52630 1 291 289 GLN . 52630 1 292 290 TRP . 52630 1 293 291 ARG . 52630 1 294 292 LYS . 52630 1 295 293 SER . 52630 1 296 294 LEU . 52630 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 52630 1 . SER 2 2 52630 1 . MET 3 3 52630 1 . GLU 4 4 52630 1 . ASP 5 5 52630 1 . SER 6 6 52630 1 . MET 7 7 52630 1 . ASP 8 8 52630 1 . MET 9 9 52630 1 . ASP 10 10 52630 1 . MET 11 11 52630 1 . SER 12 12 52630 1 . PRO 13 13 52630 1 . LEU 14 14 52630 1 . ARG 15 15 52630 1 . PRO 16 16 52630 1 . GLN 17 17 52630 1 . ASN 18 18 52630 1 . TYR 19 19 52630 1 . LEU 20 20 52630 1 . PHE 21 21 52630 1 . GLY 22 22 52630 1 . CYS 23 23 52630 1 . GLU 24 24 52630 1 . LEU 25 25 52630 1 . LYS 26 26 52630 1 . ALA 27 27 52630 1 . ASP 28 28 52630 1 . LYS 29 29 52630 1 . ASP 30 30 52630 1 . TYR 31 31 52630 1 . HIS 32 32 52630 1 . PHE 33 33 52630 1 . LYS 34 34 52630 1 . VAL 35 35 52630 1 . ASP 36 36 52630 1 . ASN 37 37 52630 1 . ASP 38 38 52630 1 . GLU 39 39 52630 1 . ASN 40 40 52630 1 . GLU 41 41 52630 1 . HIS 42 42 52630 1 . GLN 43 43 52630 1 . LEU 44 44 52630 1 . SER 45 45 52630 1 . LEU 46 46 52630 1 . ARG 47 47 52630 1 . THR 48 48 52630 1 . VAL 49 49 52630 1 . SER 50 50 52630 1 . LEU 51 51 52630 1 . GLY 52 52 52630 1 . ALA 53 53 52630 1 . GLY 54 54 52630 1 . ALA 55 55 52630 1 . LYS 56 56 52630 1 . ASP 57 57 52630 1 . GLU 58 58 52630 1 . LEU 59 59 52630 1 . HIS 60 60 52630 1 . ILE 61 61 52630 1 . VAL 62 62 52630 1 . GLU 63 63 52630 1 . ALA 64 64 52630 1 . GLU 65 65 52630 1 . ALA 66 66 52630 1 . MET 67 67 52630 1 . ASN 68 68 52630 1 . TYR 69 69 52630 1 . GLU 70 70 52630 1 . GLY 71 71 52630 1 . SER 72 72 52630 1 . PRO 73 73 52630 1 . ILE 74 74 52630 1 . LYS 75 75 52630 1 . VAL 76 76 52630 1 . THR 77 77 52630 1 . LEU 78 78 52630 1 . ALA 79 79 52630 1 . THR 80 80 52630 1 . LEU 81 81 52630 1 . LYS 82 82 52630 1 . MET 83 83 52630 1 . SER 84 84 52630 1 . VAL 85 85 52630 1 . GLN 86 86 52630 1 . PRO 87 87 52630 1 . THR 88 88 52630 1 . VAL 89 89 52630 1 . SER 90 90 52630 1 . LEU 91 91 52630 1 . GLY 92 92 52630 1 . GLY 93 93 52630 1 . PHE 94 94 52630 1 . GLU 95 95 52630 1 . ILE 96 96 52630 1 . THR 97 97 52630 1 . PRO 98 98 52630 1 . PRO 99 99 52630 1 . VAL 100 100 52630 1 . VAL 101 101 52630 1 . LEU 102 102 52630 1 . ARG 103 103 52630 1 . LEU 104 104 52630 1 . LYS 105 105 52630 1 . CYS 106 106 52630 1 . GLY 107 107 52630 1 . SER 108 108 52630 1 . GLY 109 109 52630 1 . PRO 110 110 52630 1 . VAL 111 111 52630 1 . HIS 112 112 52630 1 . ILE 113 113 52630 1 . SER 114 114 52630 1 . GLY 115 115 52630 1 . GLN 116 116 52630 1 . HIS 117 117 52630 1 . LEU 118 118 52630 1 . VAL 119 119 52630 1 . ALA 120 120 52630 1 . VAL 121 121 52630 1 . GLU 122 122 52630 1 . GLU 123 123 52630 1 . ASP 124 124 52630 1 . ALA 125 125 52630 1 . GLU 126 126 52630 1 . SER 127 127 52630 1 . GLU 128 128 52630 1 . ASP 129 129 52630 1 . GLU 130 130 52630 1 . GLU 131 131 52630 1 . GLU 132 132 52630 1 . GLU 133 133 52630 1 . ASP 134 134 52630 1 . VAL 135 135 52630 1 . LYS 136 136 52630 1 . LEU 137 137 52630 1 . LEU 138 138 52630 1 . SER 139 139 52630 1 . ILE 140 140 52630 1 . SER 141 141 52630 1 . GLY 142 142 52630 1 . LYS 143 143 52630 1 . ARG 144 144 52630 1 . SER 145 145 52630 1 . ALA 146 146 52630 1 . PRO 147 147 52630 1 . GLY 148 148 52630 1 . GLY 149 149 52630 1 . GLY 150 150 52630 1 . SER 151 151 52630 1 . LYS 152 152 52630 1 . VAL 153 153 52630 1 . PRO 154 154 52630 1 . GLN 155 155 52630 1 . LYS 156 156 52630 1 . LYS 157 157 52630 1 . VAL 158 158 52630 1 . LYS 159 159 52630 1 . LEU 160 160 52630 1 . ALA 161 161 52630 1 . ALA 162 162 52630 1 . ASP 163 163 52630 1 . GLU 164 164 52630 1 . ASP 165 165 52630 1 . ASP 166 166 52630 1 . ASP 167 167 52630 1 . ASP 168 168 52630 1 . ASP 169 169 52630 1 . ASP 170 170 52630 1 . GLU 171 171 52630 1 . GLU 172 172 52630 1 . ASP 173 173 52630 1 . ASP 174 174 52630 1 . ASP 175 175 52630 1 . GLU 176 176 52630 1 . ASP 177 177 52630 1 . ASP 178 178 52630 1 . ASP 179 179 52630 1 . ASP 180 180 52630 1 . ASP 181 181 52630 1 . ASP 182 182 52630 1 . PHE 183 183 52630 1 . ASP 184 184 52630 1 . ASP 185 185 52630 1 . GLU 186 186 52630 1 . GLU 187 187 52630 1 . ALA 188 188 52630 1 . GLU 189 189 52630 1 . GLU 190 190 52630 1 . LYS 191 191 52630 1 . ALA 192 192 52630 1 . PRO 193 193 52630 1 . VAL 194 194 52630 1 . LYS 195 195 52630 1 . LYS 196 196 52630 1 . SER 197 197 52630 1 . ILE 198 198 52630 1 . ARG 199 199 52630 1 . ASP 200 200 52630 1 . THR 201 201 52630 1 . PRO 202 202 52630 1 . ALA 203 203 52630 1 . LYS 204 204 52630 1 . ASN 205 205 52630 1 . ALA 206 206 52630 1 . GLN 207 207 52630 1 . LYS 208 208 52630 1 . SER 209 209 52630 1 . ASN 210 210 52630 1 . GLN 211 211 52630 1 . ASN 212 212 52630 1 . GLY 213 213 52630 1 . LYS 214 214 52630 1 . ASP 215 215 52630 1 . SER 216 216 52630 1 . LYS 217 217 52630 1 . PRO 218 218 52630 1 . SER 219 219 52630 1 . SER 220 220 52630 1 . THR 221 221 52630 1 . PRO 222 222 52630 1 . ARG 223 223 52630 1 . SER 224 224 52630 1 . LYS 225 225 52630 1 . GLY 226 226 52630 1 . GLN 227 227 52630 1 . GLU 228 228 52630 1 . SER 229 229 52630 1 . PHE 230 230 52630 1 . LYS 231 231 52630 1 . LYS 232 232 52630 1 . GLN 233 233 52630 1 . GLU 234 234 52630 1 . LYS 235 235 52630 1 . THR 236 236 52630 1 . PRO 237 237 52630 1 . LYS 238 238 52630 1 . THR 239 239 52630 1 . PRO 240 240 52630 1 . LYS 241 241 52630 1 . GLY 242 242 52630 1 . PRO 243 243 52630 1 . SER 244 244 52630 1 . SER 245 245 52630 1 . VAL 246 246 52630 1 . GLU 247 247 52630 1 . ASP 248 248 52630 1 . ILE 249 249 52630 1 . LYS 250 250 52630 1 . ALA 251 251 52630 1 . LYS 252 252 52630 1 . MET 253 253 52630 1 . GLN 254 254 52630 1 . ALA 255 255 52630 1 . SER 256 256 52630 1 . ILE 257 257 52630 1 . GLU 258 258 52630 1 . LYS 259 259 52630 1 . GLY 260 260 52630 1 . GLY 261 261 52630 1 . SER 262 262 52630 1 . LEU 263 263 52630 1 . PRO 264 264 52630 1 . LYS 265 265 52630 1 . VAL 266 266 52630 1 . GLU 267 267 52630 1 . ALA 268 268 52630 1 . LYS 269 269 52630 1 . PHE 270 270 52630 1 . ILE 271 271 52630 1 . ASN 272 272 52630 1 . TYR 273 273 52630 1 . VAL 274 274 52630 1 . LYS 275 275 52630 1 . ASN 276 276 52630 1 . CYS 277 277 52630 1 . PHE 278 278 52630 1 . ARG 279 279 52630 1 . MET 280 280 52630 1 . THR 281 281 52630 1 . ASP 282 282 52630 1 . GLN 283 283 52630 1 . GLU 284 284 52630 1 . ALA 285 285 52630 1 . ILE 286 286 52630 1 . GLN 287 287 52630 1 . ASP 288 288 52630 1 . LEU 289 289 52630 1 . TRP 290 290 52630 1 . GLN 291 291 52630 1 . TRP 292 292 52630 1 . ARG 293 293 52630 1 . LYS 294 294 52630 1 . SER 295 295 52630 1 . LEU 296 296 52630 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 52630 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MGSSHHHHHHSQDLENLYFQ GSMVRRFLVTLRIRRACGPP RVRVFVVHIPRLTGEWAAPG APAAVALVLMLLRSQRLGQQ PLPRRPGHDDGQRPSGGAAA APRRGAQLRRPRHSHPTRAR RCPGGLPGHAGGAAPGRGAA GRARCLGPSARGPG ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 154 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 -21 MET . 52630 2 2 -20 GLY . 52630 2 3 -19 SER . 52630 2 4 -18 SER . 52630 2 5 -17 HIS . 52630 2 6 -16 HIS . 52630 2 7 -15 HIS . 52630 2 8 -14 HIS . 52630 2 9 -13 HIS . 52630 2 10 -12 HIS . 52630 2 11 -11 SER . 52630 2 12 -10 GLN . 52630 2 13 -9 ASP . 52630 2 14 -8 LEU . 52630 2 15 -7 GLU . 52630 2 16 -6 ASN . 52630 2 17 -5 LEU . 52630 2 18 -4 TYR . 52630 2 19 -3 PHE . 52630 2 20 -2 GLN . 52630 2 21 -1 GLY . 52630 2 22 0 SER . 52630 2 23 1 MET . 52630 2 24 2 VAL . 52630 2 25 3 ARG . 52630 2 26 4 ARG . 52630 2 27 5 PHE . 52630 2 28 6 LEU . 52630 2 29 7 VAL . 52630 2 30 8 THR . 52630 2 31 9 LEU . 52630 2 32 10 ARG . 52630 2 33 11 ILE . 52630 2 34 12 ARG . 52630 2 35 13 ARG . 52630 2 36 14 ALA . 52630 2 37 15 CYS . 52630 2 38 16 GLY . 52630 2 39 17 PRO . 52630 2 40 18 PRO . 52630 2 41 19 ARG . 52630 2 42 20 VAL . 52630 2 43 21 ARG . 52630 2 44 22 VAL . 52630 2 45 23 PHE . 52630 2 46 24 VAL . 52630 2 47 25 VAL . 52630 2 48 26 HIS . 52630 2 49 27 ILE . 52630 2 50 28 PRO . 52630 2 51 29 ARG . 52630 2 52 30 LEU . 52630 2 53 31 THR . 52630 2 54 32 GLY . 52630 2 55 33 GLU . 52630 2 56 34 TRP . 52630 2 57 35 ALA . 52630 2 58 36 ALA . 52630 2 59 37 PRO . 52630 2 60 38 GLY . 52630 2 61 39 ALA . 52630 2 62 40 PRO . 52630 2 63 41 ALA . 52630 2 64 42 ALA . 52630 2 65 43 VAL . 52630 2 66 44 ALA . 52630 2 67 45 LEU . 52630 2 68 46 VAL . 52630 2 69 47 LEU . 52630 2 70 48 MET . 52630 2 71 49 LEU . 52630 2 72 50 LEU . 52630 2 73 51 ARG . 52630 2 74 52 SER . 52630 2 75 53 GLN . 52630 2 76 54 ARG . 52630 2 77 55 LEU . 52630 2 78 56 GLY . 52630 2 79 57 GLN . 52630 2 80 58 GLN . 52630 2 81 59 PRO . 52630 2 82 60 LEU . 52630 2 83 61 PRO . 52630 2 84 62 ARG . 52630 2 85 63 ARG . 52630 2 86 64 PRO . 52630 2 87 65 GLY . 52630 2 88 66 HIS . 52630 2 89 67 ASP . 52630 2 90 68 ASP . 52630 2 91 69 GLY . 52630 2 92 70 GLN . 52630 2 93 71 ARG . 52630 2 94 72 PRO . 52630 2 95 73 SER . 52630 2 96 74 GLY . 52630 2 97 75 GLY . 52630 2 98 76 ALA . 52630 2 99 77 ALA . 52630 2 100 78 ALA . 52630 2 101 79 ALA . 52630 2 102 80 PRO . 52630 2 103 81 ARG . 52630 2 104 82 ARG . 52630 2 105 83 GLY . 52630 2 106 84 ALA . 52630 2 107 85 GLN . 52630 2 108 86 LEU . 52630 2 109 87 ARG . 52630 2 110 88 ARG . 52630 2 111 89 PRO . 52630 2 112 90 ARG . 52630 2 113 91 HIS . 52630 2 114 92 SER . 52630 2 115 93 HIS . 52630 2 116 94 PRO . 52630 2 117 95 THR . 52630 2 118 96 ARG . 52630 2 119 97 ALA . 52630 2 120 98 ARG . 52630 2 121 99 ARG . 52630 2 122 100 CYS . 52630 2 123 101 PRO . 52630 2 124 102 GLY . 52630 2 125 103 GLY . 52630 2 126 104 LEU . 52630 2 127 105 PRO . 52630 2 128 106 GLY . 52630 2 129 107 HIS . 52630 2 130 108 ALA . 52630 2 131 109 GLY . 52630 2 132 110 GLY . 52630 2 133 111 ALA . 52630 2 134 112 ALA . 52630 2 135 113 PRO . 52630 2 136 114 GLY . 52630 2 137 115 ARG . 52630 2 138 116 GLY . 52630 2 139 117 ALA . 52630 2 140 118 ALA . 52630 2 141 119 GLY . 52630 2 142 120 ARG . 52630 2 143 121 ALA . 52630 2 144 122 ARG . 52630 2 145 123 CYS . 52630 2 146 124 LEU . 52630 2 147 125 GLY . 52630 2 148 126 PRO . 52630 2 149 127 SER . 52630 2 150 128 ALA . 52630 2 151 129 ARG . 52630 2 152 130 GLY . 52630 2 153 131 PRO . 52630 2 154 132 GLY . 52630 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 52630 2 . GLY 2 2 52630 2 . SER 3 3 52630 2 . SER 4 4 52630 2 . HIS 5 5 52630 2 . HIS 6 6 52630 2 . HIS 7 7 52630 2 . HIS 8 8 52630 2 . HIS 9 9 52630 2 . HIS 10 10 52630 2 . SER 11 11 52630 2 . GLN 12 12 52630 2 . ASP 13 13 52630 2 . LEU 14 14 52630 2 . GLU 15 15 52630 2 . ASN 16 16 52630 2 . LEU 17 17 52630 2 . TYR 18 18 52630 2 . PHE 19 19 52630 2 . GLN 20 20 52630 2 . GLY 21 21 52630 2 . SER 22 22 52630 2 . MET 23 23 52630 2 . VAL 24 24 52630 2 . ARG 25 25 52630 2 . ARG 26 26 52630 2 . PHE 27 27 52630 2 . LEU 28 28 52630 2 . VAL 29 29 52630 2 . THR 30 30 52630 2 . LEU 31 31 52630 2 . ARG 32 32 52630 2 . ILE 33 33 52630 2 . ARG 34 34 52630 2 . ARG 35 35 52630 2 . ALA 36 36 52630 2 . CYS 37 37 52630 2 . GLY 38 38 52630 2 . PRO 39 39 52630 2 . PRO 40 40 52630 2 . ARG 41 41 52630 2 . VAL 42 42 52630 2 . ARG 43 43 52630 2 . VAL 44 44 52630 2 . PHE 45 45 52630 2 . VAL 46 46 52630 2 . VAL 47 47 52630 2 . HIS 48 48 52630 2 . ILE 49 49 52630 2 . PRO 50 50 52630 2 . ARG 51 51 52630 2 . LEU 52 52 52630 2 . THR 53 53 52630 2 . GLY 54 54 52630 2 . GLU 55 55 52630 2 . TRP 56 56 52630 2 . ALA 57 57 52630 2 . ALA 58 58 52630 2 . PRO 59 59 52630 2 . GLY 60 60 52630 2 . ALA 61 61 52630 2 . PRO 62 62 52630 2 . ALA 63 63 52630 2 . ALA 64 64 52630 2 . VAL 65 65 52630 2 . ALA 66 66 52630 2 . LEU 67 67 52630 2 . VAL 68 68 52630 2 . LEU 69 69 52630 2 . MET 70 70 52630 2 . LEU 71 71 52630 2 . LEU 72 72 52630 2 . ARG 73 73 52630 2 . SER 74 74 52630 2 . GLN 75 75 52630 2 . ARG 76 76 52630 2 . LEU 77 77 52630 2 . GLY 78 78 52630 2 . GLN 79 79 52630 2 . GLN 80 80 52630 2 . PRO 81 81 52630 2 . LEU 82 82 52630 2 . PRO 83 83 52630 2 . ARG 84 84 52630 2 . ARG 85 85 52630 2 . PRO 86 86 52630 2 . GLY 87 87 52630 2 . HIS 88 88 52630 2 . ASP 89 89 52630 2 . ASP 90 90 52630 2 . GLY 91 91 52630 2 . GLN 92 92 52630 2 . ARG 93 93 52630 2 . PRO 94 94 52630 2 . SER 95 95 52630 2 . GLY 96 96 52630 2 . GLY 97 97 52630 2 . ALA 98 98 52630 2 . ALA 99 99 52630 2 . ALA 100 100 52630 2 . ALA 101 101 52630 2 . PRO 102 102 52630 2 . ARG 103 103 52630 2 . ARG 104 104 52630 2 . GLY 105 105 52630 2 . ALA 106 106 52630 2 . GLN 107 107 52630 2 . LEU 108 108 52630 2 . ARG 109 109 52630 2 . ARG 110 110 52630 2 . PRO 111 111 52630 2 . ARG 112 112 52630 2 . HIS 113 113 52630 2 . SER 114 114 52630 2 . HIS 115 115 52630 2 . PRO 116 116 52630 2 . THR 117 117 52630 2 . ARG 118 118 52630 2 . ALA 119 119 52630 2 . ARG 120 120 52630 2 . ARG 121 121 52630 2 . CYS 122 122 52630 2 . PRO 123 123 52630 2 . GLY 124 124 52630 2 . GLY 125 125 52630 2 . LEU 126 126 52630 2 . PRO 127 127 52630 2 . GLY 128 128 52630 2 . HIS 129 129 52630 2 . ALA 130 130 52630 2 . GLY 131 131 52630 2 . GLY 132 132 52630 2 . ALA 133 133 52630 2 . ALA 134 134 52630 2 . PRO 135 135 52630 2 . GLY 136 136 52630 2 . ARG 137 137 52630 2 . GLY 138 138 52630 2 . ALA 139 139 52630 2 . ALA 140 140 52630 2 . GLY 141 141 52630 2 . ARG 142 142 52630 2 . ALA 143 143 52630 2 . ARG 144 144 52630 2 . CYS 145 145 52630 2 . LEU 146 146 52630 2 . GLY 147 147 52630 2 . PRO 148 148 52630 2 . SER 149 149 52630 2 . ALA 150 150 52630 2 . ARG 151 151 52630 2 . GLY 152 152 52630 2 . PRO 153 153 52630 2 . GLY 154 154 52630 2 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52630 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52630 1 2 2 $entity_2 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52630 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52630 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET28 . . . 52630 1 2 2 $entity_2 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET28 . . . 52630 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52630 _Sample.ID 1 _Sample.Name na _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system na _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 NPM1 '[U-100% 13C; U-100% 15N]' . . 1 $entity_1 . . 1 . . mM . . . . 52630 1 2 p14ARF 'natural abundance' . . 2 $entity_2 . . 200 . . uM . . . . 52630 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52630 _Sample_condition_list.ID 1 _Sample_condition_list.Name na _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pressure 1 . atm 52630 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52630 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 52630 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52630 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name Bruker _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE NEO' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52630 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52630 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 52630 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name na _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 na na . . . . ppm 0 na direct 1 . . . . . 52630 1 N 15 na na . . . . ppm 0 na direct 1 . . . . . 52630 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 52630 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name NPM1_solution_assignment.txt _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 52630 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 52630 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 149 149 GLY H H 1 8.14 . . 1 . . . . . 147 GLY H . 52630 1 2 . 1 . 1 149 149 GLY N N 15 109.22 . . 1 . . . . . 147 GLY N . 52630 1 3 . 1 . 1 156 156 LYS H H 1 8.24 . . 1 . . . . . 154 LYS H . 52630 1 4 . 1 . 1 156 156 LYS N N 15 123.34 . . 1 . . . . . 154 LYS N . 52630 1 5 . 1 . 1 157 157 LYS H H 1 8.06 . . 1 . . . . . 155 LYS H . 52630 1 6 . 1 . 1 157 157 LYS N N 15 123.24 . . 1 . . . . . 155 LYS N . 52630 1 7 . 1 . 1 188 188 ALA H H 1 7.93 . . 1 . . . . . 186 ALA H . 52630 1 8 . 1 . 1 188 188 ALA N N 15 125.04 . . 1 . . . . . 186 ALA N . 52630 1 9 . 1 . 1 192 192 ALA H H 1 8.05 . . 1 . . . . . 190 ALA H . 52630 1 10 . 1 . 1 192 192 ALA N N 15 126.65 . . 1 . . . . . 190 ALA N . 52630 1 11 . 1 . 1 194 194 VAL H H 1 7.97 . . 1 . . . . . 192 VAL H . 52630 1 12 . 1 . 1 194 194 VAL N N 15 120.81 . . 1 . . . . . 192 VAL N . 52630 1 13 . 1 . 1 195 195 LYS H H 1 8.18 . . 1 . . . . . 193 LYS H . 52630 1 14 . 1 . 1 195 195 LYS N N 15 125.91 . . 1 . . . . . 193 LYS N . 52630 1 15 . 1 . 1 197 197 SER H H 1 8.2 . . 1 . . . . . 195 SER H . 52630 1 16 . 1 . 1 197 197 SER N N 15 117.65 . . 1 . . . . . 195 SER N . 52630 1 17 . 1 . 1 199 199 ARG H H 1 8.07 . . 1 . . . . . 197 ARG H . 52630 1 18 . 1 . 1 199 199 ARG N N 15 124.45 . . 1 . . . . . 197 ARG N . 52630 1 19 . 1 . 1 201 201 THR H H 1 7.83 . . 1 . . . . . 199 THR H . 52630 1 20 . 1 . 1 201 201 THR N N 15 116.67 . . 1 . . . . . 199 THR N . 52630 1 21 . 1 . 1 203 203 ALA H H 1 8.16 . . 1 . . . . . 201 ALA H . 52630 1 22 . 1 . 1 203 203 ALA N N 15 124.46 . . 1 . . . . . 201 ALA N . 52630 1 23 . 1 . 1 205 205 ASN H H 1 8.19 . . 1 . . . . . 203 ASN H . 52630 1 24 . 1 . 1 205 205 ASN N N 15 119.5 . . 1 . . . . . 203 ASN N . 52630 1 25 . 1 . 1 206 206 ALA H H 1 7.99 . . 1 . . . . . 204 ALA H . 52630 1 26 . 1 . 1 206 206 ALA N N 15 124.51 . . 1 . . . . . 204 ALA N . 52630 1 27 . 1 . 1 207 207 GLN H H 1 8.06 . . 1 . . . . . 205 GLN H . 52630 1 28 . 1 . 1 207 207 GLN N N 15 119.4 . . 1 . . . . . 205 GLN N . 52630 1 29 . 1 . 1 209 209 SER H H 1 8.13 . . 1 . . . . . 207 SER H . 52630 1 30 . 1 . 1 209 209 SER N N 15 116.91 . . 1 . . . . . 207 SER N . 52630 1 31 . 1 . 1 210 210 ASN H H 1 8.24 . . 1 . . . . . 208 ASN H . 52630 1 32 . 1 . 1 210 210 ASN N N 15 121.0 . . 1 . . . . . 208 ASN N . 52630 1 33 . 1 . 1 212 212 ASN H H 1 8.24 . . 1 . . . . . 210 ASN H . 52630 1 34 . 1 . 1 212 212 ASN N N 15 119.6 . . 1 . . . . . 210 ASN N . 52630 1 35 . 1 . 1 213 213 GLY H H 1 8.16 . . 1 . . . . . 211 GLY H . 52630 1 36 . 1 . 1 213 213 GLY N N 15 109.68 . . 1 . . . . . 211 GLY N . 52630 1 37 . 1 . 1 214 214 LYS H H 1 7.98 . . 1 . . . . . 212 LYS H . 52630 1 38 . 1 . 1 214 214 LYS N N 15 121.17 . . 1 . . . . . 212 LYS N . 52630 1 39 . 1 . 1 216 216 SER H H 1 7.92 . . 1 . . . . . 214 SER H . 52630 1 40 . 1 . 1 216 216 SER N N 15 116.35 . . 1 . . . . . 214 SER N . 52630 1 41 . 1 . 1 219 219 SER H H 1 8.25 . . 1 . . . . . 217 SER H . 52630 1 42 . 1 . 1 219 219 SER N N 15 116.5 . . 1 . . . . . 217 SER N . 52630 1 43 . 1 . 1 220 220 SER H H 1 8.15 . . 1 . . . . . 218 SER H . 52630 1 44 . 1 . 1 220 220 SER N N 15 118.17 . . 1 . . . . . 218 SER N . 52630 1 45 . 1 . 1 221 221 THR H H 1 8.0 . . 1 . . . . . 219 THR H . 52630 1 46 . 1 . 1 221 221 THR N N 15 118.69 . . 1 . . . . . 219 THR N . 52630 1 47 . 1 . 1 224 224 SER H H 1 8.13 . . 1 . . . . . 222 SER H . 52630 1 48 . 1 . 1 224 224 SER N N 15 117.4 . . 1 . . . . . 222 SER N . 52630 1 49 . 1 . 1 225 225 LYS H H 1 8.21 . . 1 . . . . . 223 LYS H . 52630 1 50 . 1 . 1 225 225 LYS N N 15 123.76 . . 1 . . . . . 223 LYS N . 52630 1 51 . 1 . 1 226 226 GLY H H 1 8.29 . . 1 . . . . . 224 GLY H . 52630 1 52 . 1 . 1 226 226 GLY N N 15 110.43 . . 1 . . . . . 224 GLY N . 52630 1 53 . 1 . 1 228 228 GLU H H 1 8.38 . . 1 . . . . . 226 GLU H . 52630 1 54 . 1 . 1 228 228 GLU N N 15 122.37 . . 1 . . . . . 226 GLU N . 52630 1 55 . 1 . 1 229 229 SER H H 1 8.05 . . 1 . . . . . 227 SER H . 52630 1 56 . 1 . 1 229 229 SER N N 15 116.71 . . 1 . . . . . 227 SER N . 52630 1 57 . 1 . 1 230 230 PHE H H 1 7.94 . . 1 . . . . . 228 PHE H . 52630 1 58 . 1 . 1 230 230 PHE N N 15 122.31 . . 1 . . . . . 228 PHE N . 52630 1 59 . 1 . 1 231 231 LYS H H 1 7.89 . . 1 . . . . . 229 LYS H . 52630 1 60 . 1 . 1 231 231 LYS N N 15 123.09 . . 1 . . . . . 229 LYS N . 52630 1 stop_ save_