data_52629 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52629 _Entry.Title ; p14ARF forms meso-scale assemblies upon phase separation with NPM1 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2024-10-04 _Entry.Accession_date 2024-10-04 _Entry.Last_release_date 2024-10-04 _Entry.Original_release_date 2024-10-04 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Eric Gibbs . . . . 52629 2 Qi Miao . . . . 52629 3 Mylene Ferrolino . . . . 52629 4 Richa Bajpai . . . . 52629 5 Aila Hassan . . . . 52629 6 Aaron Phillips . . . . 52629 7 Aaron Pitre . . . . 52629 8 Rainer Kummerle . . . . 52629 9 Shondra Miller . . . . 52629 10 Gergely Nagy . . . . 52629 11 Wellington Leite . . . . 52629 12 William Heller . . . . 52629 13 Chris Stanley . . . . 52629 14 Barbara Perrone . . . . 52629 15 Richard Kriwacki . . . . 52629 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 52629 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 44 52629 '15N chemical shifts' 18 52629 '1H chemical shifts' 17 52629 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2025-01-09 . original BMRB . 52629 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 52628 'phase separated p14ARF-NPM1 meso-scale assembly, ARF solid assignment' 52629 BMRB 52630 'phase separated p14ARF-NPM1 condensate, NPM1 solution assignment' 52629 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52629 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 39528457 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; p14ARF forms meso-scale assemblies upon phase separation with NPM1 ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Nat. Commun.' _Citation.Journal_name_full 'Nature Communications' _Citation.Journal_volume 15 _Citation.Journal_issue 1 _Citation.Journal_ASTM . _Citation.Journal_ISSN 2041-1723 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 9531 _Citation.Page_last 9531 _Citation.Year 2024 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Eric Gibbs . . . . 52629 1 2 Qi Miao . . . . 52629 1 3 Mylene Ferrolino . . . . 52629 1 4 Richa Bajpai . . . . 52629 1 5 Aila Hassan . . . . 52629 1 6 Aaron Phillips . . . . 52629 1 7 Aaron Pitre . . . . 52629 1 8 Rainer Kummerle . . . . 52629 1 9 Shondra Miller . . . . 52629 1 10 Gergely Nagy . . . . 52629 1 11 Wellington Leite . . . . 52629 1 12 William Heller . . . . 52629 1 13 Chris Stanley . . . . 52629 1 14 Barbara Perrone . . . . 52629 1 15 Richard Kriwacki . . . . 52629 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52629 _Assembly.ID 1 _Assembly.Name 'phase separated p14ARF-NPM1-IDR condensate' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 p14ARF 1 $entity_1 . . yes native no no . . . 52629 1 2 NPM1-IDR 2 $entity_2 . . no native no no . . . 52629 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52629 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MGSSHHHHHHSQDLENLYFQ GSMVRRFLVTLRIRRACGPP RVRVFVVHIPRLTGEWAAPG APAAVALVLMLLRSQRLGQQ PLPRRPGHDDGQRPSGGAAA APRRGAQLRRPRHSHPTRAR RCPGGLPGHAGGAAPGRGAA GRARCLGPSARGPG ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq ; MVRRFLVTLRIRRACGPPRV RVFVVHIPRLTGEWAAPGAP AAVALVLMLLRSQRLGQQPL PRRPGHDDGQRPSGGAAAAP RRGAQLRRPRHSHPTRARRC PGGLPGHAGGAAPGRGAAGR ARCLGPSARGPG ; _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 154 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 -21 MET . 52629 1 2 -20 GLY . 52629 1 3 -19 SER . 52629 1 4 -18 SER . 52629 1 5 -17 HIS . 52629 1 6 -16 HIS . 52629 1 7 -15 HIS . 52629 1 8 -14 HIS . 52629 1 9 -13 HIS . 52629 1 10 -12 HIS . 52629 1 11 -11 SER . 52629 1 12 -10 GLN . 52629 1 13 -9 ASP . 52629 1 14 -8 LEU . 52629 1 15 -7 GLU . 52629 1 16 -6 ASN . 52629 1 17 -5 LEU . 52629 1 18 -4 TYR . 52629 1 19 -3 PHE . 52629 1 20 -2 GLN . 52629 1 21 -1 GLY . 52629 1 22 0 SER . 52629 1 23 1 MET . 52629 1 24 2 VAL . 52629 1 25 3 ARG . 52629 1 26 4 ARG . 52629 1 27 5 PHE . 52629 1 28 6 LEU . 52629 1 29 7 VAL . 52629 1 30 8 THR . 52629 1 31 9 LEU . 52629 1 32 10 ARG . 52629 1 33 11 ILE . 52629 1 34 12 ARG . 52629 1 35 13 ARG . 52629 1 36 14 ALA . 52629 1 37 15 CYS . 52629 1 38 16 GLY . 52629 1 39 17 PRO . 52629 1 40 18 PRO . 52629 1 41 19 ARG . 52629 1 42 20 VAL . 52629 1 43 21 ARG . 52629 1 44 22 VAL . 52629 1 45 23 PHE . 52629 1 46 24 VAL . 52629 1 47 25 VAL . 52629 1 48 26 HIS . 52629 1 49 27 ILE . 52629 1 50 28 PRO . 52629 1 51 29 ARG . 52629 1 52 30 LEU . 52629 1 53 31 THR . 52629 1 54 32 GLY . 52629 1 55 33 GLU . 52629 1 56 34 TRP . 52629 1 57 35 ALA . 52629 1 58 36 ALA . 52629 1 59 37 PRO . 52629 1 60 38 GLY . 52629 1 61 39 ALA . 52629 1 62 40 PRO . 52629 1 63 41 ALA . 52629 1 64 42 ALA . 52629 1 65 43 VAL . 52629 1 66 44 ALA . 52629 1 67 45 LEU . 52629 1 68 46 VAL . 52629 1 69 47 LEU . 52629 1 70 48 MET . 52629 1 71 49 LEU . 52629 1 72 50 LEU . 52629 1 73 51 ARG . 52629 1 74 52 SER . 52629 1 75 53 GLN . 52629 1 76 54 ARG . 52629 1 77 55 LEU . 52629 1 78 56 GLY . 52629 1 79 57 GLN . 52629 1 80 58 GLN . 52629 1 81 59 PRO . 52629 1 82 60 LEU . 52629 1 83 61 PRO . 52629 1 84 62 ARG . 52629 1 85 63 ARG . 52629 1 86 64 PRO . 52629 1 87 65 GLY . 52629 1 88 66 HIS . 52629 1 89 67 ASP . 52629 1 90 68 ASP . 52629 1 91 69 GLY . 52629 1 92 70 GLN . 52629 1 93 71 ARG . 52629 1 94 72 PRO . 52629 1 95 73 SER . 52629 1 96 74 GLY . 52629 1 97 75 GLY . 52629 1 98 76 ALA . 52629 1 99 77 ALA . 52629 1 100 78 ALA . 52629 1 101 79 ALA . 52629 1 102 80 PRO . 52629 1 103 81 ARG . 52629 1 104 82 ARG . 52629 1 105 83 GLY . 52629 1 106 84 ALA . 52629 1 107 85 GLN . 52629 1 108 86 LEU . 52629 1 109 87 ARG . 52629 1 110 88 ARG . 52629 1 111 89 PRO . 52629 1 112 90 ARG . 52629 1 113 91 HIS . 52629 1 114 92 SER . 52629 1 115 93 HIS . 52629 1 116 94 PRO . 52629 1 117 95 THR . 52629 1 118 96 ARG . 52629 1 119 97 ALA . 52629 1 120 98 ARG . 52629 1 121 99 ARG . 52629 1 122 100 CYS . 52629 1 123 101 PRO . 52629 1 124 102 GLY . 52629 1 125 103 GLY . 52629 1 126 104 LEU . 52629 1 127 105 PRO . 52629 1 128 106 GLY . 52629 1 129 107 HIS . 52629 1 130 108 ALA . 52629 1 131 109 GLY . 52629 1 132 110 GLY . 52629 1 133 111 ALA . 52629 1 134 112 ALA . 52629 1 135 113 PRO . 52629 1 136 114 GLY . 52629 1 137 115 ARG . 52629 1 138 116 GLY . 52629 1 139 117 ALA . 52629 1 140 118 ALA . 52629 1 141 119 GLY . 52629 1 142 120 ARG . 52629 1 143 121 ALA . 52629 1 144 122 ARG . 52629 1 145 123 CYS . 52629 1 146 124 LEU . 52629 1 147 125 GLY . 52629 1 148 126 PRO . 52629 1 149 127 SER . 52629 1 150 128 ALA . 52629 1 151 129 ARG . 52629 1 152 130 GLY . 52629 1 153 131 PRO . 52629 1 154 132 GLY . 52629 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 52629 1 . GLY 2 2 52629 1 . SER 3 3 52629 1 . SER 4 4 52629 1 . HIS 5 5 52629 1 . HIS 6 6 52629 1 . HIS 7 7 52629 1 . HIS 8 8 52629 1 . HIS 9 9 52629 1 . HIS 10 10 52629 1 . SER 11 11 52629 1 . GLN 12 12 52629 1 . ASP 13 13 52629 1 . LEU 14 14 52629 1 . GLU 15 15 52629 1 . ASN 16 16 52629 1 . LEU 17 17 52629 1 . TYR 18 18 52629 1 . PHE 19 19 52629 1 . GLN 20 20 52629 1 . GLY 21 21 52629 1 . SER 22 22 52629 1 . MET 23 23 52629 1 . VAL 24 24 52629 1 . ARG 25 25 52629 1 . ARG 26 26 52629 1 . PHE 27 27 52629 1 . LEU 28 28 52629 1 . VAL 29 29 52629 1 . THR 30 30 52629 1 . LEU 31 31 52629 1 . ARG 32 32 52629 1 . ILE 33 33 52629 1 . ARG 34 34 52629 1 . ARG 35 35 52629 1 . ALA 36 36 52629 1 . CYS 37 37 52629 1 . GLY 38 38 52629 1 . PRO 39 39 52629 1 . PRO 40 40 52629 1 . ARG 41 41 52629 1 . VAL 42 42 52629 1 . ARG 43 43 52629 1 . VAL 44 44 52629 1 . PHE 45 45 52629 1 . VAL 46 46 52629 1 . VAL 47 47 52629 1 . HIS 48 48 52629 1 . ILE 49 49 52629 1 . PRO 50 50 52629 1 . ARG 51 51 52629 1 . LEU 52 52 52629 1 . THR 53 53 52629 1 . GLY 54 54 52629 1 . GLU 55 55 52629 1 . TRP 56 56 52629 1 . ALA 57 57 52629 1 . ALA 58 58 52629 1 . PRO 59 59 52629 1 . GLY 60 60 52629 1 . ALA 61 61 52629 1 . PRO 62 62 52629 1 . ALA 63 63 52629 1 . ALA 64 64 52629 1 . VAL 65 65 52629 1 . ALA 66 66 52629 1 . LEU 67 67 52629 1 . VAL 68 68 52629 1 . LEU 69 69 52629 1 . MET 70 70 52629 1 . LEU 71 71 52629 1 . LEU 72 72 52629 1 . ARG 73 73 52629 1 . SER 74 74 52629 1 . GLN 75 75 52629 1 . ARG 76 76 52629 1 . LEU 77 77 52629 1 . GLY 78 78 52629 1 . GLN 79 79 52629 1 . GLN 80 80 52629 1 . PRO 81 81 52629 1 . LEU 82 82 52629 1 . PRO 83 83 52629 1 . ARG 84 84 52629 1 . ARG 85 85 52629 1 . PRO 86 86 52629 1 . GLY 87 87 52629 1 . HIS 88 88 52629 1 . ASP 89 89 52629 1 . ASP 90 90 52629 1 . GLY 91 91 52629 1 . GLN 92 92 52629 1 . ARG 93 93 52629 1 . PRO 94 94 52629 1 . SER 95 95 52629 1 . GLY 96 96 52629 1 . GLY 97 97 52629 1 . ALA 98 98 52629 1 . ALA 99 99 52629 1 . ALA 100 100 52629 1 . ALA 101 101 52629 1 . PRO 102 102 52629 1 . ARG 103 103 52629 1 . ARG 104 104 52629 1 . GLY 105 105 52629 1 . ALA 106 106 52629 1 . GLN 107 107 52629 1 . LEU 108 108 52629 1 . ARG 109 109 52629 1 . ARG 110 110 52629 1 . PRO 111 111 52629 1 . ARG 112 112 52629 1 . HIS 113 113 52629 1 . SER 114 114 52629 1 . HIS 115 115 52629 1 . PRO 116 116 52629 1 . THR 117 117 52629 1 . ARG 118 118 52629 1 . ALA 119 119 52629 1 . ARG 120 120 52629 1 . ARG 121 121 52629 1 . CYS 122 122 52629 1 . PRO 123 123 52629 1 . GLY 124 124 52629 1 . GLY 125 125 52629 1 . LEU 126 126 52629 1 . PRO 127 127 52629 1 . GLY 128 128 52629 1 . HIS 129 129 52629 1 . ALA 130 130 52629 1 . GLY 131 131 52629 1 . GLY 132 132 52629 1 . ALA 133 133 52629 1 . ALA 134 134 52629 1 . PRO 135 135 52629 1 . GLY 136 136 52629 1 . ARG 137 137 52629 1 . GLY 138 138 52629 1 . ALA 139 139 52629 1 . ALA 140 140 52629 1 . GLY 141 141 52629 1 . ARG 142 142 52629 1 . ALA 143 143 52629 1 . ARG 144 144 52629 1 . CYS 145 145 52629 1 . LEU 146 146 52629 1 . GLY 147 147 52629 1 . PRO 148 148 52629 1 . SER 149 149 52629 1 . ALA 150 150 52629 1 . ARG 151 151 52629 1 . GLY 152 152 52629 1 . PRO 153 153 52629 1 . GLY 154 154 52629 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 52629 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; WEEDAESEDEEEEDVKLLSI SGKRSAPGGGSKVPQKKVKL AADEDDDDDDEEDDDEDDDD DDFDDEEAEEKAPVKKSIRD TPAKNAQKSNQGKDSKPSST PRSKGQESFKKQEKTPKTPK G ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 121 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . TRP . 52629 2 2 . GLU . 52629 2 3 . GLU . 52629 2 4 . ASP . 52629 2 5 . ALA . 52629 2 6 . GLU . 52629 2 7 . SER . 52629 2 8 . GLU . 52629 2 9 . ASP . 52629 2 10 . GLU . 52629 2 11 . GLU . 52629 2 12 . GLU . 52629 2 13 . GLU . 52629 2 14 . ASP . 52629 2 15 . VAL . 52629 2 16 . LYS . 52629 2 17 . LEU . 52629 2 18 . LEU . 52629 2 19 . SER . 52629 2 20 . ILE . 52629 2 21 . SER . 52629 2 22 . GLY . 52629 2 23 . LYS . 52629 2 24 . ARG . 52629 2 25 . SER . 52629 2 26 . ALA . 52629 2 27 . PRO . 52629 2 28 . GLY . 52629 2 29 . GLY . 52629 2 30 . GLY . 52629 2 31 . SER . 52629 2 32 . LYS . 52629 2 33 . VAL . 52629 2 34 . PRO . 52629 2 35 . GLN . 52629 2 36 . LYS . 52629 2 37 . LYS . 52629 2 38 . VAL . 52629 2 39 . LYS . 52629 2 40 . LEU . 52629 2 41 . ALA . 52629 2 42 . ALA . 52629 2 43 . ASP . 52629 2 44 . GLU . 52629 2 45 . ASP . 52629 2 46 . ASP . 52629 2 47 . ASP . 52629 2 48 . ASP . 52629 2 49 . ASP . 52629 2 50 . ASP . 52629 2 51 . GLU . 52629 2 52 . GLU . 52629 2 53 . ASP . 52629 2 54 . ASP . 52629 2 55 . ASP . 52629 2 56 . GLU . 52629 2 57 . ASP . 52629 2 58 . ASP . 52629 2 59 . ASP . 52629 2 60 . ASP . 52629 2 61 . ASP . 52629 2 62 . ASP . 52629 2 63 . PHE . 52629 2 64 . ASP . 52629 2 65 . ASP . 52629 2 66 . GLU . 52629 2 67 . GLU . 52629 2 68 . ALA . 52629 2 69 . GLU . 52629 2 70 . GLU . 52629 2 71 . LYS . 52629 2 72 . ALA . 52629 2 73 . PRO . 52629 2 74 . VAL . 52629 2 75 . LYS . 52629 2 76 . LYS . 52629 2 77 . SER . 52629 2 78 . ILE . 52629 2 79 . ARG . 52629 2 80 . ASP . 52629 2 81 . THR . 52629 2 82 . PRO . 52629 2 83 . ALA . 52629 2 84 . LYS . 52629 2 85 . ASN . 52629 2 86 . ALA . 52629 2 87 . GLN . 52629 2 88 . LYS . 52629 2 89 . SER . 52629 2 90 . ASN . 52629 2 91 . GLN . 52629 2 92 . GLY . 52629 2 93 . LYS . 52629 2 94 . ASP . 52629 2 95 . SER . 52629 2 96 . LYS . 52629 2 97 . PRO . 52629 2 98 . SER . 52629 2 99 . SER . 52629 2 100 . THR . 52629 2 101 . PRO . 52629 2 102 . ARG . 52629 2 103 . SER . 52629 2 104 . LYS . 52629 2 105 . GLY . 52629 2 106 . GLN . 52629 2 107 . GLU . 52629 2 108 . SER . 52629 2 109 . PHE . 52629 2 110 . LYS . 52629 2 111 . LYS . 52629 2 112 . GLN . 52629 2 113 . GLU . 52629 2 114 . LYS . 52629 2 115 . THR . 52629 2 116 . PRO . 52629 2 117 . LYS . 52629 2 118 . THR . 52629 2 119 . PRO . 52629 2 120 . LYS . 52629 2 121 . GLY . 52629 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . TRP 1 1 52629 2 . GLU 2 2 52629 2 . GLU 3 3 52629 2 . ASP 4 4 52629 2 . ALA 5 5 52629 2 . GLU 6 6 52629 2 . SER 7 7 52629 2 . GLU 8 8 52629 2 . ASP 9 9 52629 2 . GLU 10 10 52629 2 . GLU 11 11 52629 2 . GLU 12 12 52629 2 . GLU 13 13 52629 2 . ASP 14 14 52629 2 . VAL 15 15 52629 2 . LYS 16 16 52629 2 . LEU 17 17 52629 2 . LEU 18 18 52629 2 . SER 19 19 52629 2 . ILE 20 20 52629 2 . SER 21 21 52629 2 . GLY 22 22 52629 2 . LYS 23 23 52629 2 . ARG 24 24 52629 2 . SER 25 25 52629 2 . ALA 26 26 52629 2 . PRO 27 27 52629 2 . GLY 28 28 52629 2 . GLY 29 29 52629 2 . GLY 30 30 52629 2 . SER 31 31 52629 2 . LYS 32 32 52629 2 . VAL 33 33 52629 2 . PRO 34 34 52629 2 . GLN 35 35 52629 2 . LYS 36 36 52629 2 . LYS 37 37 52629 2 . VAL 38 38 52629 2 . LYS 39 39 52629 2 . LEU 40 40 52629 2 . ALA 41 41 52629 2 . ALA 42 42 52629 2 . ASP 43 43 52629 2 . GLU 44 44 52629 2 . ASP 45 45 52629 2 . ASP 46 46 52629 2 . ASP 47 47 52629 2 . ASP 48 48 52629 2 . ASP 49 49 52629 2 . ASP 50 50 52629 2 . GLU 51 51 52629 2 . GLU 52 52 52629 2 . ASP 53 53 52629 2 . ASP 54 54 52629 2 . ASP 55 55 52629 2 . GLU 56 56 52629 2 . ASP 57 57 52629 2 . ASP 58 58 52629 2 . ASP 59 59 52629 2 . ASP 60 60 52629 2 . ASP 61 61 52629 2 . ASP 62 62 52629 2 . PHE 63 63 52629 2 . ASP 64 64 52629 2 . ASP 65 65 52629 2 . GLU 66 66 52629 2 . GLU 67 67 52629 2 . ALA 68 68 52629 2 . GLU 69 69 52629 2 . GLU 70 70 52629 2 . LYS 71 71 52629 2 . ALA 72 72 52629 2 . PRO 73 73 52629 2 . VAL 74 74 52629 2 . LYS 75 75 52629 2 . LYS 76 76 52629 2 . SER 77 77 52629 2 . ILE 78 78 52629 2 . ARG 79 79 52629 2 . ASP 80 80 52629 2 . THR 81 81 52629 2 . PRO 82 82 52629 2 . ALA 83 83 52629 2 . LYS 84 84 52629 2 . ASN 85 85 52629 2 . ALA 86 86 52629 2 . GLN 87 87 52629 2 . LYS 88 88 52629 2 . SER 89 89 52629 2 . ASN 90 90 52629 2 . GLN 91 91 52629 2 . GLY 92 92 52629 2 . LYS 93 93 52629 2 . ASP 94 94 52629 2 . SER 95 95 52629 2 . LYS 96 96 52629 2 . PRO 97 97 52629 2 . SER 98 98 52629 2 . SER 99 99 52629 2 . THR 100 100 52629 2 . PRO 101 101 52629 2 . ARG 102 102 52629 2 . SER 103 103 52629 2 . LYS 104 104 52629 2 . GLY 105 105 52629 2 . GLN 106 106 52629 2 . GLU 107 107 52629 2 . SER 108 108 52629 2 . PHE 109 109 52629 2 . LYS 110 110 52629 2 . LYS 111 111 52629 2 . GLN 112 112 52629 2 . GLU 113 113 52629 2 . LYS 114 114 52629 2 . THR 115 115 52629 2 . PRO 116 116 52629 2 . LYS 117 117 52629 2 . THR 118 118 52629 2 . PRO 119 119 52629 2 . LYS 120 120 52629 2 . GLY 121 121 52629 2 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52629 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52629 1 2 2 $entity_2 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52629 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52629 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET28 . . . 52629 1 2 2 $entity_2 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET28 . . . 52629 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52629 _Sample.ID 1 _Sample.Name 'phase separated p14ARF-NPM1-IDR condensate' _Sample.Type 'gel solid' _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system na _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 p14ARF '[U-100% 13C; U-100% 15N]' . . 1 $entity_1 . . ~1 . . mM . . . . 52629 1 2 NPM1-IDR 'natural abundance' . . 2 $entity_2 . . ~1 . . mM . . . . 52629 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52629 _Sample_condition_list.ID 1 _Sample_condition_list.Name na _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pressure 1 . atm 52629 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52629 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 52629 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52629 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name Bruker _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE NEO' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52629 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52629 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 52629 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name na _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 na na . . . . na 0 na direct 1 . . . . . 52629 1 H 1 na na . . . . na 0 na direct 1 . . . . . 52629 1 N 15 na na . . . . na 0 na direct 1 . . . . . 52629 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 52629 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name ARF_solution_assignment_final.txt _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 52629 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 52629 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 129 129 HIS H H 1 8.155 . . 1 . . . . . 107 HIS H . 52629 1 2 . 1 . 1 129 129 HIS CA C 13 53.42 . . 1 . . . . . 107 HIS CA . 52629 1 3 . 1 . 1 129 129 HIS CB C 13 28.38 . . 1 . . . . . 107 HIS CB . 52629 1 4 . 1 . 1 129 129 HIS N N 15 119.6 . . 1 . . . . . 107 HIS N . 52629 1 5 . 1 . 1 130 130 ALA C C 13 175.4 . . 1 . . . . . 108 ALA C . 52629 1 6 . 1 . 1 130 130 ALA CA C 13 50.08 . . 1 . . . . . 108 ALA CA . 52629 1 7 . 1 . 1 130 130 ALA CB C 13 16.41 . . 1 . . . . . 108 ALA CB . 52629 1 8 . 1 . 1 131 131 GLY H H 1 8.399 . . 1 . . . . . 109 GLY H . 52629 1 9 . 1 . 1 131 131 GLY CA C 13 42.91 . . 1 . . . . . 109 GLY CA . 52629 1 10 . 1 . 1 131 131 GLY N N 15 108.3 . . 1 . . . . . 109 GLY N . 52629 1 11 . 1 . 1 132 132 GLY C C 13 170.9 . . 1 . . . . . 110 GLY C . 52629 1 12 . 1 . 1 132 132 GLY CA C 13 42.6 . . 1 . . . . . 110 GLY CA . 52629 1 13 . 1 . 1 133 133 ALA H H 1 8.124 . . 1 . . . . . 111 ALA H . 52629 1 14 . 1 . 1 133 133 ALA C C 13 174.3 . . 1 . . . . . 111 ALA C . 52629 1 15 . 1 . 1 133 133 ALA CA C 13 49.39 . . 1 . . . . . 111 ALA CA . 52629 1 16 . 1 . 1 133 133 ALA CB C 13 16.74 . . 1 . . . . . 111 ALA CB . 52629 1 17 . 1 . 1 133 133 ALA N N 15 123.5 . . 1 . . . . . 111 ALA N . 52629 1 18 . 1 . 1 134 134 ALA H H 1 8.24 . . 1 . . . . . 112 ALA H . 52629 1 19 . 1 . 1 134 134 ALA C C 13 172.8 . . 1 . . . . . 112 ALA C . 52629 1 20 . 1 . 1 134 134 ALA CA C 13 47.81 . . 1 . . . . . 112 ALA CA . 52629 1 21 . 1 . 1 134 134 ALA CB C 13 15.57 . . 1 . . . . . 112 ALA CB . 52629 1 22 . 1 . 1 134 134 ALA N N 15 124.5 . . 1 . . . . . 112 ALA N . 52629 1 23 . 1 . 1 135 135 PRO C C 13 174.9 . . 1 . . . . . 113 PRO C . 52629 1 24 . 1 . 1 135 135 PRO N N 15 135.4 . . 1 . . . . . 113 PRO N . 52629 1 25 . 1 . 1 136 136 GLY H H 1 8.42 . . 1 . . . . . 114 GLY H . 52629 1 26 . 1 . 1 136 136 GLY C C 13 171.6 . . 1 . . . . . 114 GLY C . 52629 1 27 . 1 . 1 136 136 GLY CA C 13 42.78 . . 1 . . . . . 114 GLY CA . 52629 1 28 . 1 . 1 136 136 GLY N N 15 109.0 . . 1 . . . . . 114 GLY N . 52629 1 29 . 1 . 1 137 137 ARG H H 1 8.241 . . 1 . . . . . 115 ARG H . 52629 1 30 . 1 . 1 137 137 ARG C C 13 174.3 . . 1 . . . . . 115 ARG C . 52629 1 31 . 1 . 1 137 137 ARG CA C 13 53.68 . . 1 . . . . . 115 ARG CA . 52629 1 32 . 1 . 1 137 137 ARG CB C 13 28.14 . . 1 . . . . . 115 ARG CB . 52629 1 33 . 1 . 1 137 137 ARG N N 15 120.3 . . 1 . . . . . 115 ARG N . 52629 1 34 . 1 . 1 138 138 GLY H H 1 8.505 . . 1 . . . . . 116 GLY H . 52629 1 35 . 1 . 1 138 138 GLY C C 13 171.4 . . 1 . . . . . 116 GLY C . 52629 1 36 . 1 . 1 138 138 GLY CA C 13 42.73 . . 1 . . . . . 116 GLY CA . 52629 1 37 . 1 . 1 138 138 GLY N N 15 109.9 . . 1 . . . . . 116 GLY N . 52629 1 38 . 1 . 1 139 139 ALA H H 1 8.184 . . 1 . . . . . 117 ALA H . 52629 1 39 . 1 . 1 139 139 ALA C C 13 175.2 . . 1 . . . . . 117 ALA C . 52629 1 40 . 1 . 1 139 139 ALA CA C 13 50.24 . . 1 . . . . . 117 ALA CA . 52629 1 41 . 1 . 1 139 139 ALA CB C 13 16.71 . . 1 . . . . . 117 ALA CB . 52629 1 42 . 1 . 1 139 139 ALA N N 15 123.9 . . 1 . . . . . 117 ALA N . 52629 1 43 . 1 . 1 140 140 ALA H H 1 8.289 . . 1 . . . . . 118 ALA H . 52629 1 44 . 1 . 1 140 140 ALA C C 13 175.7 . . 1 . . . . . 118 ALA C . 52629 1 45 . 1 . 1 140 140 ALA CA C 13 50.35 . . 1 . . . . . 118 ALA CA . 52629 1 46 . 1 . 1 140 140 ALA CB C 13 16.43 . . 1 . . . . . 118 ALA CB . 52629 1 47 . 1 . 1 140 140 ALA N N 15 122.6 . . 1 . . . . . 118 ALA N . 52629 1 48 . 1 . 1 141 141 GLY H H 1 8.234 . . 1 . . . . . 119 GLY H . 52629 1 49 . 1 . 1 141 141 GLY C C 13 171.5 . . 1 . . . . . 119 GLY C . 52629 1 50 . 1 . 1 141 141 GLY CA C 13 42.86 . . 1 . . . . . 119 GLY CA . 52629 1 51 . 1 . 1 141 141 GLY N N 15 107.5 . . 1 . . . . . 119 GLY N . 52629 1 52 . 1 . 1 142 142 ARG H H 1 8.053 . . 1 . . . . . 120 ARG H . 52629 1 53 . 1 . 1 142 142 ARG C C 13 172.2 . . 1 . . . . . 120 ARG C . 52629 1 54 . 1 . 1 142 142 ARG CA C 13 53.16 . . 1 . . . . . 120 ARG CA . 52629 1 55 . 1 . 1 142 142 ARG CB C 13 27.38 . . 1 . . . . . 120 ARG CB . 52629 1 56 . 1 . 1 142 142 ARG N N 15 120.3 . . 1 . . . . . 120 ARG N . 52629 1 57 . 1 . 1 143 143 ALA H H 1 8.371 . . 1 . . . . . 121 ALA H . 52629 1 58 . 1 . 1 143 143 ALA CA C 13 50.16 . . 1 . . . . . 121 ALA CA . 52629 1 59 . 1 . 1 143 143 ALA CB C 13 16.48 . . 1 . . . . . 121 ALA CB . 52629 1 60 . 1 . 1 143 143 ALA N N 15 125.4 . . 1 . . . . . 121 ALA N . 52629 1 61 . 1 . 1 147 147 GLY H H 1 8.154 . . 1 . . . . . 125 GLY H . 52629 1 62 . 1 . 1 147 147 GLY N N 15 109.4 . . 1 . . . . . 125 GLY N . 52629 1 63 . 1 . 1 149 149 SER H H 1 8.359 . . 1 . . . . . 127 SER H . 52629 1 64 . 1 . 1 149 149 SER C C 13 171.7 . . 1 . . . . . 127 SER C . 52629 1 65 . 1 . 1 149 149 SER CA C 13 56.14 . . 1 . . . . . 127 SER CA . 52629 1 66 . 1 . 1 149 149 SER CB C 13 61.12 . . 1 . . . . . 127 SER CB . 52629 1 67 . 1 . 1 149 149 SER N N 15 115.5 . . 1 . . . . . 127 SER N . 52629 1 68 . 1 . 1 150 150 ALA H H 1 8.203 . . 1 . . . . . 128 ALA H . 52629 1 69 . 1 . 1 150 150 ALA C C 13 174.8 . . 1 . . . . . 128 ALA C . 52629 1 70 . 1 . 1 150 150 ALA CA C 13 50.11 . . 1 . . . . . 128 ALA CA . 52629 1 71 . 1 . 1 150 150 ALA CB C 13 16.66 . . 1 . . . . . 128 ALA CB . 52629 1 72 . 1 . 1 150 150 ALA N N 15 125.6 . . 1 . . . . . 128 ALA N . 52629 1 73 . 1 . 1 151 151 ARG H H 1 8.246 . . 1 . . . . . 129 ARG H . 52629 1 74 . 1 . 1 151 151 ARG CA C 13 53.48 . . 1 . . . . . 129 ARG CA . 52629 1 75 . 1 . 1 151 151 ARG CB C 13 28.38 . . 1 . . . . . 129 ARG CB . 52629 1 76 . 1 . 1 151 151 ARG N N 15 119.7 . . 1 . . . . . 129 ARG N . 52629 1 77 . 1 . 1 152 152 GLY H H 1 8.15 . . 1 . . . . . 130 GLY H . 52629 1 78 . 1 . 1 152 152 GLY CA C 13 41.87 . . 1 . . . . . 130 GLY CA . 52629 1 79 . 1 . 1 152 152 GLY N N 15 109.8 . . 1 . . . . . 130 GLY N . 52629 1 stop_ save_