data_52568 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52568 _Entry.Title ; IGF2BP1 Linker2 WT ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2024-07-26 _Entry.Accession_date 2024-07-26 _Entry.Last_release_date 2024-07-26 _Entry.Original_release_date 2024-07-26 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Harald Hornegger . . . . 52568 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 52568 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 44 52568 '1H chemical shifts' 44 52568 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2024-11-05 . original BMRB . 52568 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 52567 'IGF2BP1 Linker1 WT' 52568 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52568 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 39426983 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; IGF2BP1 phosphorylation in the disordered linkers regulates ribonucleoprotein condensate formation and RNA metabolism ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Nat. Commun.' _Citation.Journal_name_full 'Nature communications' _Citation.Journal_volume 15 _Citation.Journal_issue 1 _Citation.Journal_ASTM . _Citation.Journal_ISSN 2041-1723 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 9054 _Citation.Page_last 9054 _Citation.Year 2024 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Harald Hornegger . . . . 52568 1 2 Aleksandra Anisimova . . . . 52568 1 3 Adnan Muratovic . . . . 52568 1 4 Benjamin Burgeois . . . . 52568 1 5 Elena Spinetti . . . . 52568 1 6 Isabell Niedermoser . . . . 52568 1 7 Roberto Covino . . . . 52568 1 8 Tobias Madl . . . . 52568 1 9 Elif Karagoz . . . . 52568 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52568 _Assembly.ID 1 _Assembly.Name 'IGF2BP1 Linker2 WT' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'IGF2BP1 Linker2 WT' 1 $entity_1 . . yes native no no . . . 52568 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52568 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; ATYMKKVREAYENDVAAMSL QSHLIPGLNLAAVGLFPASS SAVPPPPSSVTGAAPYSSFM QAPE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 64 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 341 ALA . 52568 1 2 342 THR . 52568 1 3 343 TYR . 52568 1 4 344 MET . 52568 1 5 345 LYS . 52568 1 6 346 LYS . 52568 1 7 347 VAL . 52568 1 8 348 ARG . 52568 1 9 349 GLU . 52568 1 10 350 ALA . 52568 1 11 351 TYR . 52568 1 12 352 GLU . 52568 1 13 353 ASN . 52568 1 14 354 ASP . 52568 1 15 355 VAL . 52568 1 16 356 ALA . 52568 1 17 357 ALA . 52568 1 18 358 MET . 52568 1 19 359 SER . 52568 1 20 360 LEU . 52568 1 21 361 GLN . 52568 1 22 362 SER . 52568 1 23 363 HIS . 52568 1 24 364 LEU . 52568 1 25 365 ILE . 52568 1 26 366 PRO . 52568 1 27 367 GLY . 52568 1 28 368 LEU . 52568 1 29 369 ASN . 52568 1 30 370 LEU . 52568 1 31 371 ALA . 52568 1 32 372 ALA . 52568 1 33 373 VAL . 52568 1 34 374 GLY . 52568 1 35 375 LEU . 52568 1 36 376 PHE . 52568 1 37 377 PRO . 52568 1 38 378 ALA . 52568 1 39 379 SER . 52568 1 40 380 SER . 52568 1 41 381 SER . 52568 1 42 382 ALA . 52568 1 43 383 VAL . 52568 1 44 384 PRO . 52568 1 45 385 PRO . 52568 1 46 386 PRO . 52568 1 47 387 PRO . 52568 1 48 388 SER . 52568 1 49 389 SER . 52568 1 50 390 VAL . 52568 1 51 391 THR . 52568 1 52 392 GLY . 52568 1 53 393 ALA . 52568 1 54 394 ALA . 52568 1 55 395 PRO . 52568 1 56 396 TYR . 52568 1 57 397 SER . 52568 1 58 398 SER . 52568 1 59 399 PHE . 52568 1 60 400 MET . 52568 1 61 401 GLN . 52568 1 62 402 ALA . 52568 1 63 403 PRO . 52568 1 64 404 GLU . 52568 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ALA 1 1 52568 1 . THR 2 2 52568 1 . TYR 3 3 52568 1 . MET 4 4 52568 1 . LYS 5 5 52568 1 . LYS 6 6 52568 1 . VAL 7 7 52568 1 . ARG 8 8 52568 1 . GLU 9 9 52568 1 . ALA 10 10 52568 1 . TYR 11 11 52568 1 . GLU 12 12 52568 1 . ASN 13 13 52568 1 . ASP 14 14 52568 1 . VAL 15 15 52568 1 . ALA 16 16 52568 1 . ALA 17 17 52568 1 . MET 18 18 52568 1 . SER 19 19 52568 1 . LEU 20 20 52568 1 . GLN 21 21 52568 1 . SER 22 22 52568 1 . HIS 23 23 52568 1 . LEU 24 24 52568 1 . ILE 25 25 52568 1 . PRO 26 26 52568 1 . GLY 27 27 52568 1 . LEU 28 28 52568 1 . ASN 29 29 52568 1 . LEU 30 30 52568 1 . ALA 31 31 52568 1 . ALA 32 32 52568 1 . VAL 33 33 52568 1 . GLY 34 34 52568 1 . LEU 35 35 52568 1 . PHE 36 36 52568 1 . PRO 37 37 52568 1 . ALA 38 38 52568 1 . SER 39 39 52568 1 . SER 40 40 52568 1 . SER 41 41 52568 1 . ALA 42 42 52568 1 . VAL 43 43 52568 1 . PRO 44 44 52568 1 . PRO 45 45 52568 1 . PRO 46 46 52568 1 . PRO 47 47 52568 1 . SER 48 48 52568 1 . SER 49 49 52568 1 . VAL 50 50 52568 1 . THR 51 51 52568 1 . GLY 52 52 52568 1 . ALA 53 53 52568 1 . ALA 54 54 52568 1 . PRO 55 55 52568 1 . TYR 56 56 52568 1 . SER 57 57 52568 1 . SER 58 58 52568 1 . PHE 59 59 52568 1 . MET 60 60 52568 1 . GLN 61 61 52568 1 . ALA 62 62 52568 1 . PRO 63 63 52568 1 . GLU 64 64 52568 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52568 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52568 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52568 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli 'BL21 RIPL' . . plasmid . . pET-21 . . . 52568 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52568 _Sample.ID 1 _Sample.Name 'IGF2BP1 Linker2 WT' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'IGF2BP1 Linker2 WT' '[U-100% 15N]' . . 1 $entity_1 . . 200 . . uM . . . . 52568 1 2 'sodium phosphate' 'natural abundance' . . . . . . 20 . . mM . . . . 52568 1 3 'sodium cloride' 'natural abundance' . . . . . . 150 . . mM . . . . 52568 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52568 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'IGF2BP1 Linker2 WT' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 7.2 . pH 52568 1 pressure 1 . atm 52568 1 temperature 298 . K 52568 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52568 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 52568 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 52568 _Software.ID 2 _Software.Type . _Software.Name NMRPipe _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 52568 2 stop_ save_ save_software_3 _Software.Sf_category software _Software.Sf_framecode software_3 _Software.Entry_ID 52568 _Software.ID 3 _Software.Type . _Software.Name CcpNMR _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 52568 3 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52568 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Bruker Avance Neo 600' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE NEO' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52568 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52568 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 52568 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'IGF2BP1 Linker2 WT' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 water protons . . . . ppm 4.773 internal direct 1 . . . . . 52568 1 N 15 water nitrogen . . . . ppm 117.083 internal indirect 1 . . . . . 52568 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 52568 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'IGF2BP1 Linker2 WT' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 52568 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 52568 1 2 $software_2 . . 52568 1 3 $software_3 . . 52568 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 5 5 LYS H H 1 8.15886658 . . . . . . . . 345 LYS H . 52568 1 2 . 1 . 1 5 5 LYS N N 15 120.4664308 . . . . . . . . 345 LYS N . 52568 1 3 . 1 . 1 6 6 LYS H H 1 8.345074634 . . . . . . . . 346 LYS H . 52568 1 4 . 1 . 1 6 6 LYS N N 15 120.3809908 . . . . . . . . 346 LYS N . 52568 1 5 . 1 . 1 7 7 VAL H H 1 8.160816402 . . . . . . . . 347 VAL H . 52568 1 6 . 1 . 1 7 7 VAL N N 15 118.9797752 . . . . . . . . 347 VAL N . 52568 1 7 . 1 . 1 8 8 ARG H H 1 8.358723391 . . . . . . . . 348 ARG H . 52568 1 8 . 1 . 1 8 8 ARG N N 15 121.4233585 . . . . . . . . 348 ARG N . 52568 1 9 . 1 . 1 9 9 GLU H H 1 8.512759373 . . . . . . . . 349 GLU H . 52568 1 10 . 1 . 1 9 9 GLU N N 15 119.4069751 . . . . . . . . 349 GLU N . 52568 1 11 . 1 . 1 10 10 ALA H H 1 8.277805755 . . . . . . . . 350 ALA H . 52568 1 12 . 1 . 1 10 10 ALA N N 15 121.1328626 . . . . . . . . 350 ALA N . 52568 1 13 . 1 . 1 11 11 TYR H H 1 7.982407638 . . . . . . . . 351 TYR H . 52568 1 14 . 1 . 1 11 11 TYR N N 15 115.3229443 . . . . . . . . 351 TYR N . 52568 1 15 . 1 . 1 13 13 ASN H H 1 8.264156998 . . . . . . . . 353 ASN H . 52568 1 16 . 1 . 1 13 13 ASN N N 15 115.8868482 . . . . . . . . 353 ASN N . 52568 1 17 . 1 . 1 14 14 ASP H H 1 8.271956288 . . . . . . . . 354 ASP H . 52568 1 18 . 1 . 1 14 14 ASP N N 15 118.1253755 . . . . . . . . 354 ASP N . 52568 1 19 . 1 . 1 15 15 VAL H H 1 8.038952492 . . . . . . . . 355 VAL H . 52568 1 20 . 1 . 1 15 15 VAL N N 15 117.7323516 . . . . . . . . 355 VAL N . 52568 1 21 . 1 . 1 16 16 ALA H H 1 8.259282441 . . . . . . . . 356 ALA H . 52568 1 22 . 1 . 1 16 16 ALA N N 15 123.012542 . . . . . . . . 356 ALA N . 52568 1 23 . 1 . 1 17 17 ALA H H 1 8.032128114 . . . . . . . . 357 ALA H . 52568 1 24 . 1 . 1 17 17 ALA N N 15 118.9968632 . . . . . . . . 357 ALA N . 52568 1 25 . 1 . 1 18 18 MET H H 1 8.117920306 . . . . . . . . 358 MET H . 52568 1 26 . 1 . 1 18 18 MET N N 15 115.2204164 . . . . . . . . 358 MET N . 52568 1 27 . 1 . 1 19 19 SER H H 1 8.152042201 . . . . . . . . 359 SER H . 52568 1 28 . 1 . 1 19 19 SER N N 15 113.101505 . . . . . . . . 359 SER N . 52568 1 29 . 1 . 1 20 20 LEU H H 1 8.094522435 . . . . . . . . 360 LEU H . 52568 1 30 . 1 . 1 20 20 LEU N N 15 120.4151668 . . . . . . . . 360 LEU N . 52568 1 31 . 1 . 1 21 21 GLN H H 1 8.207612143 . . . . . . . . 361 GLN H . 52568 1 32 . 1 . 1 21 21 GLN N N 15 116.8095999 . . . . . . . . 361 GLN N . 52568 1 33 . 1 . 1 24 24 LEU H H 1 7.900515091 . . . . . . . . 364 LEU H . 52568 1 34 . 1 . 1 24 24 LEU N N 15 119.3215351 . . . . . . . . 364 LEU N . 52568 1 35 . 1 . 1 25 25 ILE H H 1 7.99118184 . . . . . . . . 365 ILE H . 52568 1 36 . 1 . 1 25 25 ILE N N 15 120.2271989 . . . . . . . . 365 ILE N . 52568 1 37 . 1 . 1 27 27 GLY H H 1 8.440615939 . . . . . . . . 367 GLY H . 52568 1 38 . 1 . 1 27 27 GLY N N 15 106.1637791 . . . . . . . . 367 GLY N . 52568 1 39 . 1 . 1 28 28 LEU H H 1 7.930737341 . . . . . . . . 368 LEU H . 52568 1 40 . 1 . 1 28 28 LEU N N 15 118.4500474 . . . . . . . . 368 LEU N . 52568 1 41 . 1 . 1 29 29 ASN H H 1 8.472788011 . . . . . . . . 369 ASN H . 52568 1 42 . 1 . 1 29 29 ASN N N 15 116.6728959 . . . . . . . . 369 ASN N . 52568 1 43 . 1 . 1 30 30 LEU H H 1 8.193963386 . . . . . . . . 370 LEU H . 52568 1 44 . 1 . 1 30 30 LEU N N 15 119.936703 . . . . . . . . 370 LEU N . 52568 1 45 . 1 . 1 31 31 ALA H H 1 8.15886658 . . . . . . . . 371 ALA H . 52568 1 46 . 1 . 1 31 31 ALA N N 15 120.4664308 . . . . . . . . 371 ALA N . 52568 1 47 . 1 . 1 32 32 ALA H H 1 8.011654977 . . . . . . . . 372 ALA H . 52568 1 48 . 1 . 1 32 32 ALA N N 15 119.4924151 . . . . . . . . 372 ALA N . 52568 1 49 . 1 . 1 33 33 VAL H H 1 7.906364559 . . . . . . . . 373 VAL H . 52568 1 50 . 1 . 1 33 33 VAL N N 15 115.2887684 . . . . . . . . 373 VAL N . 52568 1 51 . 1 . 1 34 34 GLY H H 1 8.315827295 . . . . . . . . 374 GLY H . 52568 1 52 . 1 . 1 34 34 GLY N N 15 108.6415384 . . . . . . . . 374 GLY N . 52568 1 53 . 1 . 1 35 35 LEU H H 1 7.859568818 . . . . . . . . 375 LEU H . 52568 1 54 . 1 . 1 35 35 LEU N N 15 118.1595515 . . . . . . . . 375 LEU N . 52568 1 55 . 1 . 1 36 36 PHE H H 1 8.176414983 . . . . . . . . 376 PHE H . 52568 1 56 . 1 . 1 36 36 PHE N N 15 117.9544955 . . . . . . . . 376 PHE N . 52568 1 57 . 1 . 1 38 38 ALA H H 1 8.436716294 . . . . . . . . 378 ALA H . 52568 1 58 . 1 . 1 38 38 ALA N N 15 121.5087985 . . . . . . . . 378 ALA N . 52568 1 59 . 1 . 1 42 42 ALA H H 1 8.219994485 . . . . . . . . 382 ALA H . 52568 1 60 . 1 . 1 42 42 ALA N N 15 123.0181626 . . . . . . . . 382 ALA N . 52568 1 61 . 1 . 1 43 43 VAL H H 1 8.055525984 . . . . . . . . 383 VAL H . 52568 1 62 . 1 . 1 43 43 VAL N N 15 118.0570235 . . . . . . . . 383 VAL N . 52568 1 63 . 1 . 1 48 48 SER H H 1 8.373347061 . . . . . . . . 388 SER H . 52568 1 64 . 1 . 1 48 48 SER N N 15 112.4863372 . . . . . . . . 388 SER N . 52568 1 65 . 1 . 1 50 50 VAL H H 1 8.114020661 . . . . . . . . 390 VAL H . 52568 1 66 . 1 . 1 50 50 VAL N N 15 118.5354874 . . . . . . . . 390 VAL N . 52568 1 67 . 1 . 1 51 51 THR H H 1 8.167640781 . . . . . . . . 391 THR H . 52568 1 68 . 1 . 1 51 51 THR N N 15 114.0413447 . . . . . . . . 391 THR N . 52568 1 69 . 1 . 1 52 52 GLY H H 1 8.355139847 . . . . . . . . 392 GLY H . 52568 1 70 . 1 . 1 52 52 GLY N N 15 108.6304676 . . . . . . . . 392 GLY N . 52568 1 71 . 1 . 1 53 53 ALA H H 1 8.065275097 . . . . . . . . 393 ALA H . 52568 1 72 . 1 . 1 53 53 ALA N N 15 120.6373107 . . . . . . . . 393 ALA N . 52568 1 73 . 1 . 1 54 54 ALA H H 1 8.285605046 . . . . . . . . 394 ALA H . 52568 1 74 . 1 . 1 54 54 ALA N N 15 121.9018224 . . . . . . . . 394 ALA N . 52568 1 75 . 1 . 1 56 56 TYR H H 1 8.134493798 . . . . . . . . 396 TYR H . 52568 1 76 . 1 . 1 56 56 TYR N N 15 116.9292159 . . . . . . . . 396 TYR N . 52568 1 77 . 1 . 1 57 57 SER H H 1 8.036027759 . . . . . . . . 397 SER H . 52568 1 78 . 1 . 1 57 57 SER N N 15 114.2464007 . . . . . . . . 397 SER N . 52568 1 79 . 1 . 1 59 59 PHE H H 1 8.049676517 . . . . . . . . 399 PHE H . 52568 1 80 . 1 . 1 59 59 PHE N N 15 118.4329594 . . . . . . . . 399 PHE N . 52568 1 81 . 1 . 1 60 60 MET H H 1 8.072099476 . . . . . . . . 400 MET H . 52568 1 82 . 1 . 1 60 60 MET N N 15 118.8601593 . . . . . . . . 400 MET N . 52568 1 83 . 1 . 1 61 61 GLN H H 1 8.220326874 . . . . . . . . 401 GLN H . 52568 1 84 . 1 . 1 61 61 GLN N N 15 118.9417978 . . . . . . . . 401 GLN N . 52568 1 85 . 1 . 1 62 62 ALA H H 1 8.393820197 . . . . . . . . 402 ALA H . 52568 1 86 . 1 . 1 62 62 ALA N N 15 124.5846375 . . . . . . . . 402 ALA N . 52568 1 87 . 1 . 1 64 64 GLU H H 1 7.965834147 . . . . . . . . 404 GLU H . 52568 1 88 . 1 . 1 64 64 GLU N N 15 123.046718 . . . . . . . . 404 GLU N . 52568 1 stop_ save_