data_52558 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52558 _Entry.Title ; 1H, 15N Resonance Assignments of the Truncated Forkhead Domain of the Human Forkhead Transcription Factor FOXO4 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2024-07-18 _Entry.Accession_date 2024-07-18 _Entry.Last_release_date 2024-07-22 _Entry.Original_release_date 2024-07-22 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Donghoon Kang . . . . 52558 2 'Min June' Yang . . . . 52558 3 Chin-Ju Park . . . . 52558 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 52558 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 61 52558 '1H chemical shifts' 61 52558 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2024-11-05 . original BMRB . 52558 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52558 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 39487330 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; NMR investigation of FOXO4-DNA interaction for discriminating target and non-target DNA sequences ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Commun. Biol.' _Citation.Journal_name_full 'Communications biology' _Citation.Journal_volume 7 _Citation.Journal_issue 1 _Citation.Journal_ASTM . _Citation.Journal_ISSN 2399-3642 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 1425 _Citation.Page_last 1425 _Citation.Year 2024 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Donghoon Kang D. . . . 52558 1 2 'Min June' Yang M. J. . . 52558 1 3 Hae-Kap Cheong H. K. . . 52558 1 4 Chin-Ju Park C. J. . . 52558 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52558 _Assembly.ID 1 _Assembly.Name 'FOXO4 FHD_DW2' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'FOXO4 FHD_DW2' 1 $entity_1 . . yes native no no . . . 52558 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52558 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MKSSHHHHHHENLYFQSNAG SRRNAWGNQSYAELISQAIE SAPEKRLTLAQIYEWMVRTV PYFKDKGDSNSSAGWKNSIR HNLSLHSKFIKVHNEATGKS SWWMLNPE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 108 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 76 MET . 52558 1 2 77 LYS . 52558 1 3 78 SER . 52558 1 4 79 SER . 52558 1 5 80 HIS . 52558 1 6 81 HIS . 52558 1 7 82 HIS . 52558 1 8 83 HIS . 52558 1 9 84 HIS . 52558 1 10 85 HIS . 52558 1 11 86 GLU . 52558 1 12 87 ASN . 52558 1 13 88 LEU . 52558 1 14 89 TYR . 52558 1 15 90 PHE . 52558 1 16 91 GLN . 52558 1 17 92 SER . 52558 1 18 93 ASN . 52558 1 19 94 ALA . 52558 1 20 95 GLY . 52558 1 21 96 SER . 52558 1 22 97 ARG . 52558 1 23 98 ARG . 52558 1 24 99 ASN . 52558 1 25 100 ALA . 52558 1 26 101 TRP . 52558 1 27 102 GLY . 52558 1 28 103 ASN . 52558 1 29 104 GLN . 52558 1 30 105 SER . 52558 1 31 106 TYR . 52558 1 32 107 ALA . 52558 1 33 108 GLU . 52558 1 34 109 LEU . 52558 1 35 110 ILE . 52558 1 36 111 SER . 52558 1 37 112 GLN . 52558 1 38 113 ALA . 52558 1 39 114 ILE . 52558 1 40 115 GLU . 52558 1 41 116 SER . 52558 1 42 117 ALA . 52558 1 43 118 PRO . 52558 1 44 119 GLU . 52558 1 45 120 LYS . 52558 1 46 121 ARG . 52558 1 47 122 LEU . 52558 1 48 123 THR . 52558 1 49 124 LEU . 52558 1 50 125 ALA . 52558 1 51 126 GLN . 52558 1 52 127 ILE . 52558 1 53 128 TYR . 52558 1 54 129 GLU . 52558 1 55 130 TRP . 52558 1 56 131 MET . 52558 1 57 132 VAL . 52558 1 58 133 ARG . 52558 1 59 134 THR . 52558 1 60 135 VAL . 52558 1 61 136 PRO . 52558 1 62 137 TYR . 52558 1 63 138 PHE . 52558 1 64 139 LYS . 52558 1 65 140 ASP . 52558 1 66 141 LYS . 52558 1 67 142 GLY . 52558 1 68 143 ASP . 52558 1 69 144 SER . 52558 1 70 145 ASN . 52558 1 71 146 SER . 52558 1 72 147 SER . 52558 1 73 148 ALA . 52558 1 74 149 GLY . 52558 1 75 150 TRP . 52558 1 76 151 LYS . 52558 1 77 152 ASN . 52558 1 78 153 SER . 52558 1 79 154 ILE . 52558 1 80 155 ARG . 52558 1 81 156 HIS . 52558 1 82 157 ASN . 52558 1 83 158 LEU . 52558 1 84 159 SER . 52558 1 85 160 LEU . 52558 1 86 161 HIS . 52558 1 87 162 SER . 52558 1 88 163 LYS . 52558 1 89 164 PHE . 52558 1 90 165 ILE . 52558 1 91 166 LYS . 52558 1 92 167 VAL . 52558 1 93 168 HIS . 52558 1 94 169 ASN . 52558 1 95 170 GLU . 52558 1 96 171 ALA . 52558 1 97 172 THR . 52558 1 98 173 GLY . 52558 1 99 174 LYS . 52558 1 100 175 SER . 52558 1 101 176 SER . 52558 1 102 177 TRP . 52558 1 103 178 TRP . 52558 1 104 179 MET . 52558 1 105 180 LEU . 52558 1 106 181 ASN . 52558 1 107 182 PRO . 52558 1 108 183 GLU . 52558 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 52558 1 . LYS 2 2 52558 1 . SER 3 3 52558 1 . SER 4 4 52558 1 . HIS 5 5 52558 1 . HIS 6 6 52558 1 . HIS 7 7 52558 1 . HIS 8 8 52558 1 . HIS 9 9 52558 1 . HIS 10 10 52558 1 . GLU 11 11 52558 1 . ASN 12 12 52558 1 . LEU 13 13 52558 1 . TYR 14 14 52558 1 . PHE 15 15 52558 1 . GLN 16 16 52558 1 . SER 17 17 52558 1 . ASN 18 18 52558 1 . ALA 19 19 52558 1 . GLY 20 20 52558 1 . SER 21 21 52558 1 . ARG 22 22 52558 1 . ARG 23 23 52558 1 . ASN 24 24 52558 1 . ALA 25 25 52558 1 . TRP 26 26 52558 1 . GLY 27 27 52558 1 . ASN 28 28 52558 1 . GLN 29 29 52558 1 . SER 30 30 52558 1 . TYR 31 31 52558 1 . ALA 32 32 52558 1 . GLU 33 33 52558 1 . LEU 34 34 52558 1 . ILE 35 35 52558 1 . SER 36 36 52558 1 . GLN 37 37 52558 1 . ALA 38 38 52558 1 . ILE 39 39 52558 1 . GLU 40 40 52558 1 . SER 41 41 52558 1 . ALA 42 42 52558 1 . PRO 43 43 52558 1 . GLU 44 44 52558 1 . LYS 45 45 52558 1 . ARG 46 46 52558 1 . LEU 47 47 52558 1 . THR 48 48 52558 1 . LEU 49 49 52558 1 . ALA 50 50 52558 1 . GLN 51 51 52558 1 . ILE 52 52 52558 1 . TYR 53 53 52558 1 . GLU 54 54 52558 1 . TRP 55 55 52558 1 . MET 56 56 52558 1 . VAL 57 57 52558 1 . ARG 58 58 52558 1 . THR 59 59 52558 1 . VAL 60 60 52558 1 . PRO 61 61 52558 1 . TYR 62 62 52558 1 . PHE 63 63 52558 1 . LYS 64 64 52558 1 . ASP 65 65 52558 1 . LYS 66 66 52558 1 . GLY 67 67 52558 1 . ASP 68 68 52558 1 . SER 69 69 52558 1 . ASN 70 70 52558 1 . SER 71 71 52558 1 . SER 72 72 52558 1 . ALA 73 73 52558 1 . GLY 74 74 52558 1 . TRP 75 75 52558 1 . LYS 76 76 52558 1 . ASN 77 77 52558 1 . SER 78 78 52558 1 . ILE 79 79 52558 1 . ARG 80 80 52558 1 . HIS 81 81 52558 1 . ASN 82 82 52558 1 . LEU 83 83 52558 1 . SER 84 84 52558 1 . LEU 85 85 52558 1 . HIS 86 86 52558 1 . SER 87 87 52558 1 . LYS 88 88 52558 1 . PHE 89 89 52558 1 . ILE 90 90 52558 1 . LYS 91 91 52558 1 . VAL 92 92 52558 1 . HIS 93 93 52558 1 . ASN 94 94 52558 1 . GLU 95 95 52558 1 . ALA 96 96 52558 1 . THR 97 97 52558 1 . GLY 98 98 52558 1 . LYS 99 99 52558 1 . SER 100 100 52558 1 . SER 101 101 52558 1 . TRP 102 102 52558 1 . TRP 103 103 52558 1 . MET 104 104 52558 1 . LEU 105 105 52558 1 . ASN 106 106 52558 1 . PRO 107 107 52558 1 . GLU 108 108 52558 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52558 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52558 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52558 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . 'pET HIS TEV LIC cloning vector' . . . 52558 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52558 _Sample.ID 1 _Sample.Name 'FOXO4 FHD_DW2 (95-183 a.a.)' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'FOXO4 FHD_DW2' '[U-100% 15N]' . . 1 $entity_1 . . 160 . . uM . . . . 52558 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52558 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Room temp conditions' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 50 . mM 52558 1 pH 7.0 . pH 52558 1 pressure 1 . atm 52558 1 temperature 298 . K 52558 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52558 _Software.ID 1 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 52558 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52558 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '600 MHz Bruker NMR spectrometers' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE NEO' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52558 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '1D 15N' yes . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52558 1 stop_ loop_ _Experiment_file.Experiment_ID _Experiment_file.Experiment_name _Experiment_file.Name _Experiment_file.Type _Experiment_file.Content _Experiment_file.Directory_path _Experiment_file.Details _Experiment_file.Entry_ID _Experiment_file.Experiment_list_ID 1 '1D 15N' 2rr . 'Time-domain data' . . 52558 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 52558 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name dss _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm -0.0376 na direct 1 . . . . . 52558 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 52558 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'FOXO4 FHD_DW2 (95-183 a.a.)' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '1D 15N' . . . 52558 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 52558 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 22 22 ARG H H 1 8.265 0.000 . . . . . . . 97 ARG H . 52558 1 2 . 1 . 1 22 22 ARG N N 15 122.983 0.000 . . . . . . . 97 ARG N . 52558 1 3 . 1 . 1 27 27 GLY H H 1 7.401 0.000 . . . . . . . 102 GLY H . 52558 1 4 . 1 . 1 27 27 GLY N N 15 109.759 0.000 . . . . . . . 102 GLY N . 52558 1 5 . 1 . 1 29 29 GLN H H 1 8.284 0.000 . . . . . . . 104 GLN H . 52558 1 6 . 1 . 1 29 29 GLN N N 15 119.537 0.000 . . . . . . . 104 GLN N . 52558 1 7 . 1 . 1 30 30 SER H H 1 8.839 0.000 . . . . . . . 105 SER H . 52558 1 8 . 1 . 1 30 30 SER N N 15 118.983 0.000 . . . . . . . 105 SER N . 52558 1 9 . 1 . 1 32 32 ALA H H 1 8.585 0.000 . . . . . . . 107 ALA H . 52558 1 10 . 1 . 1 32 32 ALA N N 15 119.121 0.000 . . . . . . . 107 ALA N . 52558 1 11 . 1 . 1 33 33 GLU H H 1 7.903 0.000 . . . . . . . 108 GLU H . 52558 1 12 . 1 . 1 33 33 GLU N N 15 119.628 0.000 . . . . . . . 108 GLU N . 52558 1 13 . 1 . 1 34 34 LEU H H 1 7.923 0.000 . . . . . . . 109 LEU H . 52558 1 14 . 1 . 1 34 34 LEU N N 15 121.064 0.000 . . . . . . . 109 LEU N . 52558 1 15 . 1 . 1 35 35 ILE H H 1 8.417 0.000 . . . . . . . 110 ILE H . 52558 1 16 . 1 . 1 35 35 ILE N N 15 117.717 0.000 . . . . . . . 110 ILE N . 52558 1 17 . 1 . 1 36 36 SER H H 1 7.867 0.000 . . . . . . . 111 SER H . 52558 1 18 . 1 . 1 36 36 SER N N 15 113.068 0.000 . . . . . . . 111 SER N . 52558 1 19 . 1 . 1 37 37 GLN H H 1 7.639 0.000 . . . . . . . 112 GLN H . 52558 1 20 . 1 . 1 37 37 GLN N N 15 120.503 0.000 . . . . . . . 112 GLN N . 52558 1 21 . 1 . 1 38 38 ALA H H 1 7.312 0.000 . . . . . . . 113 ALA H . 52558 1 22 . 1 . 1 38 38 ALA N N 15 123.715 0.000 . . . . . . . 113 ALA N . 52558 1 23 . 1 . 1 39 39 ILE H H 1 7.719 0.000 . . . . . . . 114 ILE H . 52558 1 24 . 1 . 1 39 39 ILE N N 15 117.766 0.000 . . . . . . . 114 ILE N . 52558 1 25 . 1 . 1 40 40 GLU H H 1 8.301 0.000 . . . . . . . 115 GLU H . 52558 1 26 . 1 . 1 40 40 GLU N N 15 116.260 0.000 . . . . . . . 115 GLU N . 52558 1 27 . 1 . 1 41 41 SER H H 1 7.285 0.000 . . . . . . . 116 SER H . 52558 1 28 . 1 . 1 41 41 SER N N 15 111.513 0.000 . . . . . . . 116 SER N . 52558 1 29 . 1 . 1 42 42 ALA H H 1 7.405 0.000 . . . . . . . 117 ALA H . 52558 1 30 . 1 . 1 42 42 ALA N N 15 128.342 0.000 . . . . . . . 117 ALA N . 52558 1 31 . 1 . 1 45 45 LYS H H 1 8.112 0.000 . . . . . . . 120 LYS H . 52558 1 32 . 1 . 1 45 45 LYS N N 15 113.791 0.000 . . . . . . . 120 LYS N . 52558 1 33 . 1 . 1 46 46 ARG H H 1 6.662 0.000 . . . . . . . 121 ARG H . 52558 1 34 . 1 . 1 46 46 ARG N N 15 109.557 0.000 . . . . . . . 121 ARG N . 52558 1 35 . 1 . 1 47 47 LEU H H 1 8.564 0.000 . . . . . . . 122 LEU H . 52558 1 36 . 1 . 1 47 47 LEU N N 15 120.171 0.000 . . . . . . . 122 LEU N . 52558 1 37 . 1 . 1 48 48 THR H H 1 8.844 0.000 . . . . . . . 123 THR H . 52558 1 38 . 1 . 1 48 48 THR N N 15 112.422 0.000 . . . . . . . 123 THR N . 52558 1 39 . 1 . 1 49 49 LEU H H 1 9.283 0.000 . . . . . . . 124 LEU H . 52558 1 40 . 1 . 1 49 49 LEU N N 15 122.231 0.000 . . . . . . . 124 LEU N . 52558 1 41 . 1 . 1 50 50 ALA H H 1 8.239 0.000 . . . . . . . 125 ALA H . 52558 1 42 . 1 . 1 50 50 ALA N N 15 117.785 0.000 . . . . . . . 125 ALA N . 52558 1 43 . 1 . 1 51 51 GLN H H 1 7.594 0.000 . . . . . . . 126 GLN H . 52558 1 44 . 1 . 1 51 51 GLN N N 15 116.328 0.000 . . . . . . . 126 GLN N . 52558 1 45 . 1 . 1 52 52 ILE H H 1 8.310 0.000 . . . . . . . 127 ILE H . 52558 1 46 . 1 . 1 52 52 ILE N N 15 124.804 0.000 . . . . . . . 127 ILE N . 52558 1 47 . 1 . 1 53 53 TYR H H 1 8.084 0.000 . . . . . . . 128 TYR H . 52558 1 48 . 1 . 1 53 53 TYR N N 15 118.326 0.000 . . . . . . . 128 TYR N . 52558 1 49 . 1 . 1 54 54 GLU H H 1 8.095 0.000 . . . . . . . 129 GLU H . 52558 1 50 . 1 . 1 54 54 GLU N N 15 117.565 0.000 . . . . . . . 129 GLU N . 52558 1 51 . 1 . 1 55 55 TRP H H 1 8.180 0.000 . . . . . . . 130 TRP H . 52558 1 52 . 1 . 1 55 55 TRP N N 15 120.664 0.000 . . . . . . . 130 TRP N . 52558 1 53 . 1 . 1 56 56 MET H H 1 8.289 0.000 . . . . . . . 131 MET H . 52558 1 54 . 1 . 1 56 56 MET N N 15 119.694 0.000 . . . . . . . 131 MET N . 52558 1 55 . 1 . 1 57 57 VAL H H 1 7.480 0.000 . . . . . . . 132 VAL H . 52558 1 56 . 1 . 1 57 57 VAL N N 15 115.824 0.000 . . . . . . . 132 VAL N . 52558 1 57 . 1 . 1 58 58 ARG H H 1 7.632 0.000 . . . . . . . 133 ARG H . 52558 1 58 . 1 . 1 58 58 ARG N N 15 116.759 0.000 . . . . . . . 133 ARG N . 52558 1 59 . 1 . 1 59 59 THR H H 1 7.438 0.000 . . . . . . . 134 THR H . 52558 1 60 . 1 . 1 59 59 THR N N 15 111.725 0.000 . . . . . . . 134 THR N . 52558 1 61 . 1 . 1 60 60 VAL H H 1 7.924 0.000 . . . . . . . 135 VAL H . 52558 1 62 . 1 . 1 60 60 VAL N N 15 124.594 0.000 . . . . . . . 135 VAL N . 52558 1 63 . 1 . 1 62 62 TYR H H 1 7.974 0.000 . . . . . . . 137 TYR H . 52558 1 64 . 1 . 1 62 62 TYR N N 15 115.902 0.000 . . . . . . . 137 TYR N . 52558 1 65 . 1 . 1 63 63 PHE H H 1 7.363 0.000 . . . . . . . 138 PHE H . 52558 1 66 . 1 . 1 63 63 PHE N N 15 113.138 0.000 . . . . . . . 138 PHE N . 52558 1 67 . 1 . 1 64 64 LYS H H 1 7.402 0.000 . . . . . . . 139 LYS H . 52558 1 68 . 1 . 1 64 64 LYS N N 15 120.188 0.000 . . . . . . . 139 LYS N . 52558 1 69 . 1 . 1 65 65 ASP H H 1 8.035 0.000 . . . . . . . 140 ASP H . 52558 1 70 . 1 . 1 65 65 ASP N N 15 116.543 0.000 . . . . . . . 140 ASP N . 52558 1 71 . 1 . 1 66 66 LYS H H 1 7.678 0.000 . . . . . . . 141 LYS H . 52558 1 72 . 1 . 1 66 66 LYS N N 15 119.514 0.000 . . . . . . . 141 LYS N . 52558 1 73 . 1 . 1 67 67 GLY H H 1 8.488 0.000 . . . . . . . 142 GLY H . 52558 1 74 . 1 . 1 67 67 GLY N N 15 108.099 0.000 . . . . . . . 142 GLY N . 52558 1 75 . 1 . 1 68 68 ASP H H 1 8.123 0.000 . . . . . . . 143 ASP H . 52558 1 76 . 1 . 1 68 68 ASP N N 15 119.338 0.000 . . . . . . . 143 ASP N . 52558 1 77 . 1 . 1 73 73 ALA H H 1 7.478 0.000 . . . . . . . 148 ALA H . 52558 1 78 . 1 . 1 73 73 ALA N N 15 124.042 0.000 . . . . . . . 148 ALA N . 52558 1 79 . 1 . 1 77 77 ASN H H 1 7.719 0.000 . . . . . . . 152 ASN H . 52558 1 80 . 1 . 1 77 77 ASN N N 15 119.084 0.000 . . . . . . . 152 ASN N . 52558 1 81 . 1 . 1 78 78 SER H H 1 8.067 0.000 . . . . . . . 153 SER H . 52558 1 82 . 1 . 1 78 78 SER N N 15 117.414 0.000 . . . . . . . 153 SER N . 52558 1 83 . 1 . 1 79 79 ILE H H 1 8.161 0.000 . . . . . . . 154 ILE H . 52558 1 84 . 1 . 1 79 79 ILE N N 15 124.839 0.000 . . . . . . . 154 ILE N . 52558 1 85 . 1 . 1 80 80 ARG H H 1 7.682 0.000 . . . . . . . 155 ARG H . 52558 1 86 . 1 . 1 80 80 ARG N N 15 118.801 0.000 . . . . . . . 155 ARG N . 52558 1 87 . 1 . 1 81 81 HIS H H 1 8.318 0.000 . . . . . . . 156 HIS H . 52558 1 88 . 1 . 1 81 81 HIS N N 15 117.998 0.000 . . . . . . . 156 HIS N . 52558 1 89 . 1 . 1 82 82 ASN H H 1 7.880 0.000 . . . . . . . 157 ASN H . 52558 1 90 . 1 . 1 82 82 ASN N N 15 117.136 0.000 . . . . . . . 157 ASN N . 52558 1 91 . 1 . 1 83 83 LEU H H 1 7.813 0.000 . . . . . . . 158 LEU H . 52558 1 92 . 1 . 1 83 83 LEU N N 15 119.845 0.000 . . . . . . . 158 LEU N . 52558 1 93 . 1 . 1 84 84 SER H H 1 7.024 0.000 . . . . . . . 159 SER H . 52558 1 94 . 1 . 1 84 84 SER N N 15 109.334 0.000 . . . . . . . 159 SER N . 52558 1 95 . 1 . 1 85 85 LEU H H 1 7.413 0.000 . . . . . . . 160 LEU H . 52558 1 96 . 1 . 1 85 85 LEU N N 15 120.762 0.000 . . . . . . . 160 LEU N . 52558 1 97 . 1 . 1 88 88 LYS H H 1 7.740 0.000 . . . . . . . 163 LYS H . 52558 1 98 . 1 . 1 88 88 LYS N N 15 119.272 0.000 . . . . . . . 163 LYS N . 52558 1 99 . 1 . 1 89 89 PHE H H 1 7.733 0.000 . . . . . . . 164 PHE H . 52558 1 100 . 1 . 1 89 89 PHE N N 15 118.573 0.000 . . . . . . . 164 PHE N . 52558 1 101 . 1 . 1 90 90 ILE H H 1 9.212 0.000 . . . . . . . 165 ILE H . 52558 1 102 . 1 . 1 90 90 ILE N N 15 119.925 0.000 . . . . . . . 165 ILE N . 52558 1 103 . 1 . 1 91 91 LYS H H 1 7.983 0.000 . . . . . . . 166 LYS H . 52558 1 104 . 1 . 1 91 91 LYS N N 15 125.733 0.000 . . . . . . . 166 LYS N . 52558 1 105 . 1 . 1 92 92 VAL H H 1 8.923 0.000 . . . . . . . 167 VAL H . 52558 1 106 . 1 . 1 92 92 VAL N N 15 126.861 0.000 . . . . . . . 167 VAL N . 52558 1 107 . 1 . 1 93 93 HIS H H 1 8.378 0.000 . . . . . . . 168 HIS H . 52558 1 108 . 1 . 1 93 93 HIS N N 15 124.569 0.000 . . . . . . . 168 HIS N . 52558 1 109 . 1 . 1 96 96 ALA H H 1 8.232 0.000 . . . . . . . 171 ALA H . 52558 1 110 . 1 . 1 96 96 ALA N N 15 122.412 0.000 . . . . . . . 171 ALA N . 52558 1 111 . 1 . 1 97 97 THR H H 1 7.758 0.000 . . . . . . . 172 THR H . 52558 1 112 . 1 . 1 97 97 THR N N 15 112.102 0.000 . . . . . . . 172 THR N . 52558 1 113 . 1 . 1 102 102 TRP H H 1 8.280 0.000 . . . . . . . 177 TRP H . 52558 1 114 . 1 . 1 102 102 TRP N N 15 120.198 0.000 . . . . . . . 177 TRP N . 52558 1 115 . 1 . 1 103 103 TRP H H 1 9.096 0.000 . . . . . . . 178 TRP H . 52558 1 116 . 1 . 1 103 103 TRP N N 15 121.701 0.000 . . . . . . . 178 TRP N . 52558 1 117 . 1 . 1 104 104 MET H H 1 9.170 0.000 . . . . . . . 179 MET H . 52558 1 118 . 1 . 1 104 104 MET N N 15 118.128 0.000 . . . . . . . 179 MET N . 52558 1 119 . 1 . 1 105 105 LEU H H 1 9.079 0.000 . . . . . . . 180 LEU H . 52558 1 120 . 1 . 1 105 105 LEU N N 15 120.387 0.000 . . . . . . . 180 LEU N . 52558 1 121 . 1 . 1 108 108 GLU H H 1 7.906 0.000 . . . . . . . 183 GLU H . 52558 1 122 . 1 . 1 108 108 GLU N N 15 119.383 0.000 . . . . . . . 183 GLU N . 52558 1 stop_ save_