data_27221 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C and 15N Chemical Shift Assignments for residues 421-470 of human PABPC1 ; _BMRB_accession_number 27221 _BMRB_flat_file_name bmr27221.str _Entry_type original _Submission_date 2017-08-16 _Accession_date 2017-08-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Imai Shunsuke . . 2 Sawazaki Ryoichi . . 3 Yokogawa Mariko . . 4 Shimada Ichio . . 5 Osawa Masanori . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 41 "13C chemical shifts" 114 "15N chemical shifts" 41 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-03-08 original BMRB . stop_ _Original_release_date 2017-08-17 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29362417 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sawazaki Ryoichi . . 2 Imai Shunsuke . . 3 Yokogawa Mariko . . 4 Hosoda Nao . . 5 Hoshino Shin-ichi . . 6 Mio Muneyo . . 7 Mio Kazuhiro . . 8 Shimada Ichio . . 9 Osawa Masanori . . stop_ _Journal_abbreviation 'Sci. Rep.' _Journal_name_full 'Scientific reports' _Journal_volume 8 _Journal_issue 1 _Journal_ISSN 2045-2322 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1455 _Page_last 1455 _Year 2018 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Residues 421-470 of PABPC1' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Residues 421-470 of PABPC1' $Residues_421-470_of_human_PABPC1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Residues_421-470_of_human_PABPC1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Residues_421-470_of_human_PABPC1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 55 _Mol_residue_sequence ; GPLGSAYYPPSQIAQLRPSP RWTAQGARPHPFQNMPGAIR PAAPRPPFSTMRPAS ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 GLY 2 2 PRO 3 3 LEU 4 4 GLY 5 5 SER 6 421 ALA 7 422 TYR 8 423 TYR 9 424 PRO 10 425 PRO 11 426 SER 12 427 GLN 13 428 ILE 14 429 ALA 15 430 GLN 16 431 LEU 17 432 ARG 18 433 PRO 19 434 SER 20 435 PRO 21 436 ARG 22 437 TRP 23 438 THR 24 439 ALA 25 440 GLN 26 441 GLY 27 442 ALA 28 443 ARG 29 444 PRO 30 445 HIS 31 446 PRO 32 447 PHE 33 448 GLN 34 449 ASN 35 450 MET 36 451 PRO 37 452 GLY 38 453 ALA 39 454 ILE 40 455 ARG 41 456 PRO 42 457 ALA 43 458 ALA 44 459 PRO 45 460 ARG 46 461 PRO 47 462 PRO 48 463 PHE 49 464 SER 50 465 THR 51 466 MET 52 467 ARG 53 468 PRO 54 469 ALA 55 470 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Residues_421-470_of_human_PABPC1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Residues_421-470_of_human_PABPC1 'recombinant technology' . Escherichia coli . pET-42b(+) stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Residues_421-470_of_human_PABPC1 290 uM '[U-13C; U-15N]' 'sodium phosphate' 26 mM 'natural abundance' 'sodium chloride' 100 mM 'natural abundance' EDTA 0.5 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version 'ver 3.6' loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ save_CARA _Saveframe_category software _Name CARA _Version 'ver 1.5.1' loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 500 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_CC(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CC(CO)NH' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HNCO_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_15N-edited_TOCSY_HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '15N-edited TOCSY HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 6.0 . pH temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio TSP C 13 'methyl protons' ppm 0 internal indirect . . . 0.25144954 TSP H 1 'methyl protons' ppm 0 internal direct . . . 1 TSP N 15 'methyl protons' ppm 0 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $TOPSPIN $xwinnmr $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D CBCA(CO)NH' '3D CC(CO)NH' '3D HN(CO)CA' '3D HNCO' '15N-edited TOCSY HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Residues 421-470 of PABPC1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 LEU H H 8.430 0.020 1 2 3 3 LEU C C 175.276 0.400 1 3 3 3 LEU CA C 52.738 0.400 1 4 3 3 LEU CB C 39.725 0.400 1 5 3 3 LEU N N 122.346 0.400 1 6 4 4 GLY H H 8.339 0.020 1 7 4 4 GLY C C 171.562 0.400 1 8 4 4 GLY CA C 42.667 0.400 1 9 4 4 GLY N N 109.801 0.400 1 10 5 5 SER H H 8.109 0.020 1 11 5 5 SER CA C 55.787 0.400 1 12 5 5 SER CB C 61.390 0.400 1 13 5 5 SER N N 115.490 0.400 1 14 421 6 ALA H H 8.195 0.020 1 15 421 6 ALA C C 174.000 0.400 1 16 421 6 ALA CA C 49.759 0.400 1 17 421 6 ALA CB C 16.354 0.400 1 18 421 6 ALA N N 125.393 0.400 1 19 422 7 TYR H H 7.921 0.020 1 20 422 7 TYR C C 171.794 0.400 1 21 422 7 TYR CA C 55.149 0.400 1 22 422 7 TYR CB C 36.425 0.400 1 23 422 7 TYR N N 119.553 0.400 1 24 423 8 TYR H H 7.718 0.020 1 25 423 8 TYR C C 169.821 0.400 1 26 423 8 TYR CA C 52.241 0.400 1 27 423 8 TYR CB C 37.773 0.400 1 28 423 8 TYR N N 124.682 0.400 1 29 426 11 SER H H 8.231 0.020 1 30 426 11 SER CA C 55.787 0.400 1 31 426 11 SER CB C 61.319 0.400 1 32 426 11 SER N N 115.098 0.400 1 33 427 12 GLN H H 8.300 0.020 1 34 427 12 GLN C C 173.380 0.400 1 35 427 12 GLN CA C 53.234 0.400 1 36 427 12 GLN CB C 26.851 0.400 1 37 427 12 GLN N N 121.883 0.400 1 38 428 13 ILE H H 7.921 0.020 1 39 428 13 ILE C C 173.458 0.400 1 40 428 13 ILE CA C 58.908 0.400 1 41 428 13 ILE CB C 35.929 0.400 1 42 428 13 ILE N N 120.975 0.400 1 43 429 14 ALA H H 8.195 0.020 1 44 429 14 ALA C C 174.915 0.400 1 45 429 14 ALA CA C 50.114 0.400 1 46 429 14 ALA CB C 16.213 0.400 1 47 429 14 ALA N N 126.968 0.400 1 48 430 15 GLN H H 8.104 0.020 1 49 430 15 GLN C C 173.110 0.400 1 50 430 15 GLN CA C 53.163 0.400 1 51 430 15 GLN CB C 26.709 0.400 1 52 430 15 GLN N N 118.740 0.400 1 53 431 16 LEU H H 8.082 0.020 1 54 431 16 LEU C C 174.257 0.400 1 55 431 16 LEU CA C 52.738 0.400 1 56 431 16 LEU CB C 39.725 0.400 1 57 431 16 LEU N N 123.057 0.400 1 58 432 17 ARG H H 8.184 0.020 1 59 432 17 ARG C C 171.227 0.400 1 60 432 17 ARG CA C 51.036 0.400 1 61 432 17 ARG CB C 27.418 0.400 1 62 432 17 ARG N N 122.549 0.400 1 63 434 19 SER H H 8.280 0.020 1 64 434 19 SER C C 170.247 0.400 1 65 434 19 SER CA C 53.802 0.400 1 66 434 19 SER CB C 60.877 0.400 1 67 434 19 SER N N 117.572 0.400 1 68 436 21 ARG H H 8.195 0.020 1 69 436 21 ARG C C 173.406 0.400 1 70 436 21 ARG CA C 53.802 0.400 1 71 436 21 ARG CB C 27.844 0.400 1 72 436 21 ARG N N 120.365 0.400 1 73 437 22 TRP H H 7.996 0.020 1 74 437 22 TRP C C 173.587 0.400 1 75 437 22 TRP CA C 54.724 0.400 1 76 437 22 TRP CB C 26.993 0.400 1 77 437 22 TRP N N 121.533 0.400 1 78 438 23 THR H H 7.739 0.020 1 79 438 23 THR C C 171.098 0.400 1 80 438 23 THR CA C 58.837 0.400 1 81 438 23 THR CB C 67.561 0.400 1 82 438 23 THR N N 115.693 0.400 1 83 439 24 ALA H H 8.013 0.020 1 84 439 24 ALA C C 175.083 0.400 1 85 439 24 ALA CA C 49.972 0.400 1 86 439 24 ALA CB C 16.071 0.400 1 87 439 24 ALA N N 125.647 0.400 1 88 440 25 GLN H H 8.200 0.020 1 89 440 25 GLN C C 173.896 0.400 1 90 440 25 GLN CA C 53.589 0.400 1 91 440 25 GLN CB C 26.567 0.400 1 92 440 25 GLN N N 118.994 0.400 1 93 441 26 GLY H H 8.243 0.020 1 94 441 26 GLY C C 170.917 0.400 1 95 441 26 GLY CA C 42.454 0.400 1 96 441 26 GLY N N 109.801 0.400 1 97 442 27 ALA H H 8.013 0.020 1 98 442 27 ALA C C 174.748 0.400 1 99 442 27 ALA CA C 49.617 0.400 1 100 442 27 ALA CB C 16.496 0.400 1 101 442 27 ALA N N 123.412 0.400 1 102 443 28 ARG H H 8.162 0.020 1 103 443 28 ARG C C 171.498 0.400 1 104 443 28 ARG CA C 51.036 0.400 1 105 443 28 ARG CB C 27.418 0.400 1 106 443 28 ARG N N 121.178 0.400 1 107 445 30 HIS H H 8.336 0.020 1 108 445 30 HIS C C 170.569 0.400 1 109 445 30 HIS N N 119.720 0.400 1 110 447 32 PHE H H 8.344 0.020 1 111 447 32 PHE C C 172.955 0.400 1 112 447 32 PHE CA C 55.007 0.400 1 113 447 32 PHE CB C 36.355 0.400 1 114 447 32 PHE N N 8.344 0.400 1 115 448 33 GLN H H 8.109 0.020 1 116 448 33 GLN C C 172.387 0.400 1 117 448 33 GLN CA C 53.163 0.400 1 118 448 33 GLN CB C 26.993 0.400 1 119 448 33 GLN N N 121.482 0.400 1 120 449 34 ASN H H 8.337 0.020 1 121 449 34 ASN C C 171.936 0.400 1 122 449 34 ASN CA C 51.036 0.400 1 123 449 34 ASN CB C 36.355 0.400 1 124 449 34 ASN N N 119.664 0.400 1 125 450 35 MET H H 8.168 0.020 1 126 450 35 MET C C 171.394 0.400 1 127 450 35 MET CA C 50.681 0.400 1 128 450 35 MET CB C 29.688 0.400 1 129 450 35 MET N N 121.533 0.400 1 130 452 37 GLY H H 8.425 0.020 1 131 452 37 GLY C C 171.124 0.400 1 132 452 37 GLY CA C 42.525 0.400 1 133 452 37 GLY N N 109.446 0.400 1 134 453 38 ALA H H 7.938 0.020 1 135 453 38 ALA C C 174.864 0.400 1 136 453 38 ALA CA C 49.688 0.400 1 137 453 38 ALA CB C 16.638 0.400 1 138 453 38 ALA N N 123.260 0.400 1 139 454 39 ILE H H 8.055 0.020 1 140 454 39 ILE C C 173.316 0.400 1 141 454 39 ILE CA C 58.336 0.400 1 142 454 39 ILE CB C 35.858 0.400 1 143 454 39 ILE N N 120.162 0.400 1 144 455 40 ARG H H 8.323 0.020 1 145 455 40 ARG C C 171.227 0.400 1 146 455 40 ARG CA C 51.036 0.400 1 147 455 40 ARG CB C 27.418 0.400 1 148 455 40 ARG N N 126.663 0.400 1 149 457 42 ALA H H 8.296 0.020 1 150 457 42 ALA C C 174.348 0.400 1 151 457 42 ALA CA C 49.475 0.400 1 152 457 42 ALA CB C 16.496 0.400 1 153 457 42 ALA N N 124.479 0.400 1 154 458 43 ALA H H 8.162 0.020 1 155 458 43 ALA C C 172.749 0.400 1 156 458 43 ALA CA C 47.631 0.400 1 157 458 43 ALA CB C 15.503 0.400 1 158 458 43 ALA N N 124.631 0.400 1 159 460 45 ARG H H 8.304 0.020 1 160 460 45 ARG C C 171.201 0.400 1 161 460 45 ARG CA C 51.134 0.400 1 162 460 45 ARG CB C 27.124 0.400 1 163 460 45 ARG N N 122.291 0.400 1 164 463 48 PHE H H 8.039 0.020 1 165 463 48 PHE C C 173.097 0.400 1 166 463 48 PHE CA C 55.078 0.400 1 167 463 48 PHE CB C 36.709 0.400 1 168 463 48 PHE N N 119.146 0.400 1 169 464 49 SER H H 8.109 0.020 1 170 464 49 SER CA C 55.433 0.400 1 171 464 49 SER CB C 61.461 0.400 1 172 464 49 SER N N 116.708 0.400 1 173 465 50 THR H H 8.093 0.020 1 174 465 50 THR CA C 59.335 0.400 1 175 465 50 THR CB C 66.966 0.400 1 176 465 50 THR N N 115.449 0.400 1 177 466 51 MET H H 8.181 0.020 1 178 466 51 MET C C 173.006 0.400 1 179 466 51 MET CA C 52.809 0.400 1 180 466 51 MET CB C 30.184 0.400 1 181 466 51 MET N N 122.348 0.400 1 182 467 52 ARG H H 8.248 0.020 1 183 467 52 ARG C C 171.265 0.400 1 184 467 52 ARG CA C 51.248 0.400 1 185 467 52 ARG CB C 27.489 0.400 1 186 467 52 ARG N N 123.666 0.400 1 187 469 54 ALA H H 8.387 0.020 1 188 469 54 ALA C C 174.245 0.400 1 189 469 54 ALA CA C 49.901 0.400 1 190 469 54 ALA CB C 16.425 0.400 1 191 469 54 ALA N N 124.936 0.400 1 192 470 55 SER H H 7.809 0.020 1 193 470 55 SER C C 175.882 0.400 1 194 470 55 SER CA C 57.066 0.400 1 195 470 55 SER CB C 62.312 0.400 1 196 470 55 SER N N 120.264 0.400 1 stop_ save_