data_26906 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone chemical shift assignment of the C-terminal domain of Respiratory Syncytial Virus phosphoprotein ; _BMRB_accession_number 26906 _BMRB_flat_file_name bmr26906.str _Entry_type original _Submission_date 2016-09-22 _Accession_date 2016-09-22 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lassoued Safa . . 2 Pereira Nelson . . 3 Fix Jenna . . 4 Galloux Marie . . 5 Sizun Christina . . 6 Eleouet Jean-Francois . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 159 "13C chemical shifts" 238 "15N chemical shifts" 81 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2017-02-15 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 26902 P1-126 26903 P_delta[122-160] 26904 P1-163_tetramer 26905 P-S23C_tetramer stop_ _Original_release_date 2016-09-22 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; New Insights into Structural Disorder in Human Respiratory Syncytial Virus Phosphoprotein and Implications for Binding of Protein Partners ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 28031463 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Pereira Nelson . . 2 Cardone Christophe . . 3 Lassoued Safa . . 4 Galloux Marie . . 5 Fix Jenna . . 6 Assrir Nadine . . 7 Lescop Ewen . . 8 Bontems Francois . . 9 Eleouet Jean-Francois . . 10 Sizun Christina . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 292 _Journal_issue 6 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2120 _Page_last 2131 _Year 2017 _Details . loop_ _Keyword 'Human Respiratory Syncytial Virus' 'Intrinsically Disordered Protein' Phosphoprotein 'RNA Polymerase' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name P-CTD _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label P-CTD $P-CTD stop_ _System_molecular_weight 9323.1498 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_P-CTD _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common P-CTD _Molecular_mass 9323.1498 _Mol_thiol_state 'not present' loop_ _Biological_function 'Nucleocapsid-binding domain of hRSV phosphoprotein' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 83 _Mol_residue_sequence ; GSSARDGIRDAMVGLREEMI EKIRTEALMTNDRLEAMARL RNEESEKMAKDTSDEVSLNP TSEKLNNLLEGNDSDNDLSL EDF ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 159 GLY 2 160 SER 3 161 SER 4 162 ALA 5 163 ARG 6 164 ASP 7 165 GLY 8 166 ILE 9 167 ARG 10 168 ASP 11 169 ALA 12 170 MET 13 171 VAL 14 172 GLY 15 173 LEU 16 174 ARG 17 175 GLU 18 176 GLU 19 177 MET 20 178 ILE 21 179 GLU 22 180 LYS 23 181 ILE 24 182 ARG 25 183 THR 26 184 GLU 27 185 ALA 28 186 LEU 29 187 MET 30 188 THR 31 189 ASN 32 190 ASP 33 191 ARG 34 192 LEU 35 193 GLU 36 194 ALA 37 195 MET 38 196 ALA 39 197 ARG 40 198 LEU 41 199 ARG 42 200 ASN 43 201 GLU 44 202 GLU 45 203 SER 46 204 GLU 47 205 LYS 48 206 MET 49 207 ALA 50 208 LYS 51 209 ASP 52 210 THR 53 211 SER 54 212 ASP 55 213 GLU 56 214 VAL 57 215 SER 58 216 LEU 59 217 ASN 60 218 PRO 61 219 THR 62 220 SER 63 221 GLU 64 222 LYS 65 223 LEU 66 224 ASN 67 225 ASN 68 226 LEU 69 227 LEU 70 228 GLU 71 229 GLY 72 230 ASN 73 231 ASP 74 232 SER 75 233 ASP 76 234 ASN 77 235 ASP 78 236 LEU 79 237 SER 80 238 LEU 81 239 GLU 82 240 ASP 83 241 PHE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value UniProt P12579 P12579 . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $P-CTD 'human RSV' 11250 Viruses . Orthopneumovirus HRSV Long stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $P-CTD 'recombinant technology' 'Escherichia coli' Escherichia coli BL21(DE3) pGEX stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_P-CTD_15N13C _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $P-CTD 0.15 mM '[U-13C; U-15N; U-2H]' 'sodium phosphate' 20.00 mM 'natural abundance' 'soudim chloride' 100.00 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CcpNmr_Analysis _Saveframe_category software _Name CcpNmr_Analysis _Version 2.2 loop_ _Vendor _Address _Electronic_address CCPN 'Department of Biochemistry, Cambridge CB2 1GA, UK' http://www.ccpn.ac.uk stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details 'The CCPN NMR assignment and data analysis application' save_ save_Topspin _Saveframe_category software _Name Topspin _Version 3.1 loop_ _Vendor _Address _Electronic_address Bruker . . stop_ loop_ _Task collection processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600MHz _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $P-CTD_15N13C save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $P-CTD_15N13C save_ save_3D_HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $P-CTD_15N13C save_ save_3D_HNHA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNHA' _Sample_label $P-CTD_15N13C save_ save_3D_HNCO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $P-CTD_15N13C save_ save_3D_HNCACB_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $P-CTD_15N13C save_ save_3D_CBCA(CO)NH_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $P-CTD_15N13C save_ ####################### # Sample conditions # ####################### save_CondSet1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.100 0.01 M pH 6.500 0.10 pH pressure 1.000 0.01 atm temperature 288.000 0.20 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CcpNmr_Analysis stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HN(CO)CA' '3D HNHA' '3D HNCO' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $P-CTD_15N13C stop_ _Sample_conditions_label $CondSet1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name P-CTD _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 161 3 SER H H 8.65 0.02 1 2 161 3 SER C C 174.36 0.20 1 3 161 3 SER CA C 58.23 0.20 1 4 161 3 SER CB C 63.86 0.20 1 5 161 3 SER N N 118.39 0.15 1 6 162 4 ALA H H 8.50 0.02 1 7 162 4 ALA C C 177.94 0.20 1 8 162 4 ALA CA C 52.81 0.20 1 9 162 4 ALA CB C 19.01 0.20 1 10 162 4 ALA N N 126.03 0.15 1 11 163 5 ARG H H 8.37 0.02 1 12 163 5 ARG HA H 4.32 0.02 1 13 163 5 ARG C C 176.16 0.20 1 14 163 5 ARG CA C 56.17 0.20 1 15 163 5 ARG CB C 30.39 0.20 1 16 163 5 ARG N N 119.61 0.15 1 17 164 6 ASP H H 8.29 0.02 1 18 164 6 ASP HA H 4.57 0.02 1 19 164 6 ASP C C 176.58 0.20 1 20 164 6 ASP CA C 54.55 0.20 1 21 164 6 ASP CB C 41.00 0.20 1 22 164 6 ASP N N 120.77 0.15 1 23 165 7 GLY H H 8.37 0.02 1 24 165 7 GLY HA2 H 3.95 0.02 1 25 165 7 GLY C C 174.21 0.20 1 26 165 7 GLY CA C 45.40 0.20 1 27 165 7 GLY N N 108.88 0.15 1 28 166 8 ILE H H 8.01 0.02 1 29 166 8 ILE HA H 4.14 0.02 1 30 166 8 ILE C C 176.52 0.20 1 31 166 8 ILE CA C 61.38 0.20 1 32 166 8 ILE CB C 38.23 0.20 1 33 166 8 ILE N N 120.50 0.15 1 34 167 9 ARG H H 8.47 0.02 1 35 167 9 ARG HA H 4.32 0.02 1 36 167 9 ARG C C 176.20 0.20 1 37 167 9 ARG CA C 56.34 0.20 1 38 167 9 ARG CB C 30.66 0.20 1 39 167 9 ARG N N 124.80 0.15 1 40 168 10 ASP H H 8.36 0.02 1 41 168 10 ASP HA H 4.53 0.02 1 42 168 10 ASP C C 176.19 0.20 1 43 168 10 ASP CA C 54.51 0.20 1 44 168 10 ASP CB C 41.08 0.20 1 45 168 10 ASP N N 121.30 0.15 1 46 169 11 ALA H H 8.29 0.02 1 47 169 11 ALA HA H 4.28 0.02 1 48 169 11 ALA C C 177.97 0.20 1 49 169 11 ALA CA C 52.92 0.20 1 50 169 11 ALA CB C 19.12 0.20 1 51 169 11 ALA N N 123.99 0.15 1 52 170 12 MET H H 8.37 0.02 1 53 170 12 MET HA H 4.47 0.02 1 54 170 12 MET C C 176.56 0.20 1 55 170 12 MET CA C 55.61 0.20 1 56 170 12 MET CB C 32.42 0.20 1 57 170 12 MET N N 118.21 0.15 1 58 171 13 VAL H H 8.00 0.02 1 59 171 13 VAL HA H 4.05 0.02 1 60 171 13 VAL C C 176.71 0.20 1 61 171 13 VAL CA C 63.10 0.20 1 62 171 13 VAL CB C 32.40 0.20 1 63 171 13 VAL N N 120.77 0.15 1 64 172 14 GLY H H 8.52 0.02 1 65 172 14 GLY HA2 H 3.95 0.02 1 66 172 14 GLY C C 174.14 0.20 1 67 172 14 GLY CA C 45.23 0.20 1 68 172 14 GLY N N 111.77 0.15 1 69 173 15 LEU H H 8.07 0.02 1 70 173 15 LEU HA H 4.34 0.02 1 71 173 15 LEU C C 177.70 0.20 1 72 173 15 LEU CA C 55.46 0.20 1 73 173 15 LEU CB C 42.39 0.20 1 74 173 15 LEU N N 121.62 0.15 1 75 174 16 ARG H H 8.39 0.02 1 76 174 16 ARG HA H 4.27 0.02 1 77 174 16 ARG C C 176.94 0.20 1 78 174 16 ARG CA C 56.76 0.20 1 79 174 16 ARG CB C 30.46 0.20 1 80 174 16 ARG N N 121.45 0.15 1 81 175 17 GLU H H 8.63 0.02 1 82 175 17 GLU HA H 4.16 0.02 1 83 175 17 GLU C C 177.27 0.20 1 84 175 17 GLU CA C 57.91 0.20 1 85 175 17 GLU CB C 29.83 0.20 1 86 175 17 GLU N N 121.40 0.15 1 87 176 18 GLU H H 8.59 0.02 1 88 176 18 GLU HA H 4.23 0.02 1 89 176 18 GLU C C 177.61 0.20 1 90 176 18 GLU CA C 57.57 0.20 1 91 176 18 GLU CB C 29.83 0.20 1 92 176 18 GLU N N 120.77 0.15 1 93 177 19 MET H H 8.26 0.02 1 94 177 19 MET HA H 4.42 0.02 1 95 177 19 MET C C 176.81 0.20 1 96 177 19 MET CA C 56.26 0.20 1 97 177 19 MET CB C 32.15 0.20 1 98 177 19 MET N N 120.56 0.15 1 99 178 20 ILE H H 8.06 0.02 1 100 178 20 ILE HA H 3.98 0.02 1 101 178 20 ILE C C 176.97 0.20 1 102 178 20 ILE CA C 62.56 0.20 1 103 178 20 ILE CB C 38.29 0.20 1 104 178 20 ILE N N 121.42 0.15 1 105 179 21 GLU H H 8.33 0.02 1 106 179 21 GLU HA H 4.28 0.02 1 107 179 21 GLU C C 177.09 0.20 1 108 179 21 GLU CA C 57.37 0.20 1 109 179 21 GLU CB C 29.80 0.20 1 110 179 21 GLU N N 122.92 0.15 1 111 180 22 LYS H H 8.18 0.02 1 112 180 22 LYS HA H 4.22 0.02 1 113 180 22 LYS C C 177.36 0.20 1 114 180 22 LYS CA C 57.56 0.20 1 115 180 22 LYS CB C 32.70 0.20 1 116 180 22 LYS N N 122.12 0.15 1 117 181 23 ILE H H 8.10 0.02 1 118 181 23 ILE HA H 4.24 0.02 1 119 181 23 ILE C C 177.21 0.20 1 120 181 23 ILE CA C 62.48 0.20 1 121 181 23 ILE CB C 38.28 0.20 1 122 181 23 ILE N N 120.94 0.15 1 123 182 24 ARG H H 8.36 0.02 1 124 182 24 ARG HA H 4.33 0.02 1 125 182 24 ARG C C 177.36 0.20 1 126 182 24 ARG CA C 57.29 0.20 1 127 182 24 ARG CB C 30.61 0.20 1 128 182 24 ARG N N 123.74 0.15 1 129 183 25 THR H H 8.31 0.02 1 130 183 25 THR HA H 4.19 0.02 1 131 183 25 THR C C 175.40 0.20 1 132 183 25 THR CA C 63.42 0.20 1 133 183 25 THR CB C 69.41 0.20 1 134 183 25 THR N N 115.06 0.15 1 135 184 26 GLU H H 8.46 0.02 1 136 184 26 GLU HA H 4.22 0.02 1 137 184 26 GLU C C 176.98 0.20 1 138 184 26 GLU CA C 57.57 0.20 1 139 184 26 GLU CB C 29.80 0.20 1 140 184 26 GLU N N 122.70 0.15 1 141 185 27 ALA H H 8.20 0.02 1 142 185 27 ALA HA H 4.23 0.02 1 143 185 27 ALA C C 178.15 0.20 1 144 185 27 ALA CA C 53.09 0.20 1 145 185 27 ALA CB C 18.81 0.20 1 146 185 27 ALA N N 123.49 0.15 1 147 186 28 LEU H H 8.00 0.02 1 148 186 28 LEU HA H 4.28 0.02 1 149 186 28 LEU C C 177.76 0.20 1 150 186 28 LEU CA C 55.62 0.20 1 151 186 28 LEU CB C 42.14 0.20 1 152 186 28 LEU N N 120.00 0.15 1 153 187 29 MET H H 8.29 0.02 1 154 187 29 MET HA H 4.55 0.02 1 155 187 29 MET C C 176.73 0.20 1 156 187 29 MET CA C 55.71 0.20 1 157 187 29 MET CB C 32.60 0.20 1 158 187 29 MET N N 120.35 0.15 1 159 188 30 THR H H 8.07 0.02 1 160 188 30 THR HA H 4.30 0.02 1 161 188 30 THR C C 174.77 0.20 1 162 188 30 THR CA C 62.45 0.20 1 163 188 30 THR CB C 69.52 0.20 1 164 188 30 THR N N 114.73 0.15 1 165 189 31 ASN H H 8.62 0.02 1 166 189 31 ASN C C 175.90 0.20 1 167 189 31 ASN CA C 54.72 0.20 1 168 189 31 ASN CB C 38.34 0.20 1 169 189 31 ASN N N 120.39 0.15 1 170 190 32 ASP H H 8.46 0.02 1 171 190 32 ASP HA H 4.47 0.02 1 172 190 32 ASP C C 177.52 0.20 1 173 190 32 ASP CA C 55.76 0.20 1 174 190 32 ASP CB C 40.54 0.20 1 175 190 32 ASP N N 119.92 0.15 1 176 191 33 ARG H H 8.10 0.02 1 177 191 33 ARG HA H 4.24 0.02 1 178 191 33 ARG C C 177.74 0.20 1 179 191 33 ARG CA C 57.80 0.20 1 180 191 33 ARG CB C 30.12 0.20 1 181 191 33 ARG N N 121.12 0.15 1 182 192 34 LEU H H 8.19 0.02 1 183 192 34 LEU HA H 4.20 0.02 1 184 192 34 LEU C C 179.48 0.20 1 185 192 34 LEU CA C 57.13 0.20 1 186 192 34 LEU CB C 41.58 0.20 1 187 192 34 LEU N N 121.14 0.15 1 188 193 35 GLU H H 8.42 0.02 1 189 193 35 GLU HA H 4.14 0.02 1 190 193 35 GLU C C 178.06 0.20 1 191 193 35 GLU CA C 58.30 0.20 1 192 193 35 GLU CB C 29.38 0.20 1 193 193 35 GLU N N 120.71 0.15 1 194 194 36 ALA H H 8.10 0.02 1 195 194 36 ALA HA H 4.15 0.02 1 196 194 36 ALA C C 179.71 0.20 1 197 194 36 ALA CA C 54.30 0.20 1 198 194 36 ALA CB C 18.48 0.20 1 199 194 36 ALA N N 122.66 0.15 1 200 195 37 MET H H 8.16 0.02 1 201 195 37 MET HA H 4.29 0.02 1 202 195 37 MET C C 177.63 0.20 1 203 195 37 MET CA C 57.23 0.20 1 204 195 37 MET CB C 32.73 0.20 1 205 195 37 MET N N 117.46 0.15 1 206 196 38 ALA H H 8.02 0.02 1 207 196 38 ALA HA H 4.18 0.02 1 208 196 38 ALA C C 178.95 0.20 1 209 196 38 ALA CA C 53.97 0.20 1 210 196 38 ALA CB C 18.46 0.20 1 211 196 38 ALA N N 123.10 0.15 1 212 197 39 ARG H H 8.00 0.02 1 213 197 39 ARG HA H 4.21 0.02 1 214 197 39 ARG C C 177.54 0.20 1 215 197 39 ARG CA C 57.70 0.20 1 216 197 39 ARG CB C 30.34 0.20 1 217 197 39 ARG N N 118.41 0.15 1 218 198 40 LEU H H 7.92 0.02 1 219 198 40 LEU HA H 4.27 0.02 1 220 198 40 LEU C C 178.13 0.20 1 221 198 40 LEU CA C 56.25 0.20 1 222 198 40 LEU CB C 41.83 0.20 1 223 198 40 LEU N N 120.92 0.15 1 224 199 41 ARG H H 8.12 0.02 1 225 199 41 ARG HA H 4.25 0.02 1 226 199 41 ARG C C 177.06 0.20 1 227 199 41 ARG CA C 57.07 0.20 1 228 199 41 ARG CB C 30.48 0.20 1 229 199 41 ARG N N 119.91 0.15 1 230 200 42 ASN H H 8.35 0.02 1 231 200 42 ASN HA H 4.64 0.02 1 232 200 42 ASN C C 175.91 0.20 1 233 200 42 ASN CA C 54.09 0.20 1 234 200 42 ASN CB C 38.65 0.20 1 235 200 42 ASN N N 119.28 0.15 1 236 201 43 GLU H H 8.48 0.02 1 237 201 43 GLU HA H 4.21 0.02 1 238 201 43 GLU C C 177.51 0.20 1 239 201 43 GLU CA C 57.74 0.20 1 240 201 43 GLU CB C 29.83 0.20 1 241 201 43 GLU N N 120.99 0.15 1 242 202 44 GLU H H 8.38 0.02 1 243 202 44 GLU HA H 4.23 0.02 1 244 202 44 GLU C C 177.50 0.20 1 245 202 44 GLU CA C 57.53 0.20 1 246 202 44 GLU CB C 29.79 0.20 1 247 202 44 GLU N N 120.43 0.15 1 248 203 45 SER H H 8.25 0.02 1 249 203 45 SER HA H 4.35 0.02 1 250 203 45 SER C C 175.42 0.20 1 251 203 45 SER CA C 59.65 0.20 1 252 203 45 SER CB C 63.36 0.20 1 253 203 45 SER N N 115.88 0.15 1 254 204 46 GLU H H 8.33 0.02 1 255 204 46 GLU HA H 4.25 0.02 1 256 204 46 GLU C C 177.01 0.20 1 257 204 46 GLU CA C 57.37 0.20 1 258 204 46 GLU CB C 29.94 0.20 1 259 204 46 GLU N N 122.12 0.15 1 260 205 47 LYS H H 8.11 0.02 1 261 205 47 LYS HA H 4.00 0.02 1 262 205 47 LYS C C 176.93 0.20 1 263 205 47 LYS CA C 56.91 0.20 1 264 205 47 LYS CB C 32.76 0.20 1 265 205 47 LYS N N 120.91 0.15 1 266 206 48 MET H H 8.20 0.02 1 267 206 48 MET HA H 4.41 0.02 1 268 206 48 MET C C 176.17 0.20 1 269 206 48 MET CA C 55.59 0.20 1 270 206 48 MET CB C 32.76 0.20 1 271 206 48 MET N N 120.16 0.15 1 272 207 49 ALA H H 8.24 0.02 1 273 207 49 ALA HA H 4.31 0.02 1 274 207 49 ALA C C 177.67 0.20 1 275 207 49 ALA CA C 52.55 0.20 1 276 207 49 ALA CB C 19.06 0.20 1 277 207 49 ALA N N 124.78 0.15 1 278 208 50 LYS H H 8.26 0.02 1 279 208 50 LYS HA H 4.34 0.02 1 280 208 50 LYS C C 176.27 0.20 1 281 208 50 LYS CA C 56.16 0.20 1 282 208 50 LYS CB C 33.03 0.20 1 283 208 50 LYS N N 120.41 0.15 1 284 209 51 ASP H H 8.48 0.02 1 285 209 51 ASP HA H 4.68 0.02 1 286 209 51 ASP C C 176.54 0.20 1 287 209 51 ASP CA C 54.33 0.20 1 288 209 51 ASP CB C 41.13 0.20 1 289 209 51 ASP N N 121.67 0.15 1 290 210 52 THR H H 8.19 0.02 1 291 210 52 THR HA H 4.40 0.02 1 292 210 52 THR C C 174.74 0.20 1 293 210 52 THR CA C 61.61 0.20 1 294 210 52 THR CB C 69.51 0.20 1 295 210 52 THR N N 114.21 0.15 1 296 211 53 SER H H 8.45 0.02 1 297 211 53 SER HA H 4.44 0.02 1 298 211 53 SER C C 174.33 0.20 1 299 211 53 SER CA C 58.77 0.20 1 300 211 53 SER CB C 63.69 0.20 1 301 211 53 SER N N 118.06 0.15 1 302 212 54 ASP H H 8.45 0.02 1 303 212 54 ASP HA H 4.63 0.02 1 304 212 54 ASP C C 176.14 0.20 1 305 212 54 ASP CA C 54.37 0.20 1 306 212 54 ASP CB C 41.00 0.20 1 307 212 54 ASP N N 122.21 0.15 1 308 213 55 GLU H H 8.31 0.02 1 309 213 55 GLU HA H 4.28 0.02 1 310 213 55 GLU C C 176.57 0.20 1 311 213 55 GLU CA C 56.67 0.20 1 312 213 55 GLU CB C 30.24 0.20 1 313 213 55 GLU N N 120.92 0.15 1 314 214 56 VAL H H 8.21 0.02 1 315 214 56 VAL HA H 4.10 0.02 1 316 214 56 VAL C C 176.19 0.20 1 317 214 56 VAL CA C 62.44 0.20 1 318 214 56 VAL CB C 32.52 0.20 1 319 214 56 VAL N N 121.21 0.15 1 320 215 57 SER H H 8.42 0.02 1 321 215 57 SER HA H 4.44 0.02 1 322 215 57 SER C C 174.31 0.20 1 323 215 57 SER CA C 58.12 0.20 1 324 215 57 SER CB C 63.58 0.20 1 325 215 57 SER N N 119.30 0.15 1 326 216 58 LEU H H 8.36 0.02 1 327 216 58 LEU HA H 4.36 0.02 1 328 216 58 LEU C C 176.70 0.20 1 329 216 58 LEU CA C 54.90 0.20 1 330 216 58 LEU CB C 42.38 0.20 1 331 216 58 LEU N N 124.24 0.15 1 332 217 59 ASN H H 8.45 0.02 1 333 217 59 ASN HA H 4.98 0.02 1 334 217 59 ASN CA C 51.17 0.20 1 335 217 59 ASN CB C 38.72 0.20 1 336 217 59 ASN N N 119.96 0.15 1 337 218 60 PRO C C 177.23 0.20 1 338 218 60 PRO CA C 63.37 0.20 1 339 218 60 PRO CB C 32.00 0.20 1 340 219 61 THR H H 8.32 0.02 1 341 219 61 THR HA H 4.37 0.02 1 342 219 61 THR C C 174.63 0.20 1 343 219 61 THR CA C 61.88 0.20 1 344 219 61 THR CB C 69.60 0.20 1 345 219 61 THR N N 113.75 0.15 1 346 220 62 SER H H 8.31 0.02 1 347 220 62 SER HA H 4.42 0.02 1 348 220 62 SER C C 174.52 0.20 1 349 220 62 SER CA C 58.46 0.20 1 350 220 62 SER CB C 63.55 0.20 1 351 220 62 SER N N 117.68 0.15 1 352 221 63 GLU H H 8.51 0.02 1 353 221 63 GLU HA H 4.27 0.02 1 354 221 63 GLU C C 176.33 0.20 1 355 221 63 GLU CA C 56.73 0.20 1 356 221 63 GLU CB C 30.05 0.20 1 357 221 63 GLU N N 123.07 0.15 1 358 222 64 LYS H H 8.34 0.02 1 359 222 64 LYS HA H 4.27 0.02 1 360 222 64 LYS C C 176.47 0.20 1 361 222 64 LYS CA C 56.28 0.20 1 362 222 64 LYS CB C 32.69 0.20 1 363 222 64 LYS N N 122.11 0.15 1 364 223 65 LEU H H 8.29 0.02 1 365 223 65 LEU HA H 4.33 0.02 1 366 223 65 LEU C C 177.09 0.20 1 367 223 65 LEU CA C 55.11 0.20 1 368 223 65 LEU CB C 42.08 0.20 1 369 223 65 LEU N N 123.03 0.15 1 370 224 66 ASN H H 8.45 0.02 1 371 224 66 ASN HA H 4.66 0.02 1 372 224 66 ASN C C 174.83 0.20 1 373 224 66 ASN CA C 53.10 0.20 1 374 224 66 ASN CB C 38.70 0.20 1 375 224 66 ASN N N 119.01 0.15 1 376 225 67 ASN H H 8.42 0.02 1 377 225 67 ASN HA H 4.68 0.02 1 378 225 67 ASN C C 175.11 0.20 1 379 225 67 ASN CA C 53.26 0.20 1 380 225 67 ASN CB C 38.58 0.20 1 381 225 67 ASN N N 119.05 0.15 1 382 226 68 LEU H H 8.25 0.02 1 383 226 68 LEU HA H 4.32 0.02 1 384 226 68 LEU C C 177.31 0.20 1 385 226 68 LEU CA C 55.41 0.20 1 386 226 68 LEU CB C 42.03 0.20 1 387 226 68 LEU N N 121.86 0.15 1 388 227 69 LEU H H 8.19 0.02 1 389 227 69 LEU HA H 4.37 0.02 1 390 227 69 LEU C C 177.22 0.20 1 391 227 69 LEU CA C 54.99 0.20 1 392 227 69 LEU CB C 41.94 0.20 1 393 227 69 LEU N N 121.85 0.15 1 394 228 70 GLU H H 8.31 0.02 1 395 228 70 GLU HA H 4.32 0.02 1 396 228 70 GLU C C 176.72 0.20 1 397 228 70 GLU CA C 56.46 0.20 1 398 228 70 GLU CB C 30.33 0.20 1 399 228 70 GLU N N 121.18 0.15 1 400 229 71 GLY H H 8.47 0.02 1 401 229 71 GLY HA2 H 3.98 0.02 1 402 229 71 GLY C C 173.75 0.20 1 403 229 71 GLY CA C 45.13 0.20 1 404 229 71 GLY N N 109.77 0.15 1 405 230 72 ASN H H 8.41 0.02 1 406 230 72 ASN HA H 4.77 0.02 1 407 230 72 ASN C C 174.98 0.20 1 408 230 72 ASN CA C 52.96 0.20 1 409 230 72 ASN CB C 39.24 0.20 1 410 230 72 ASN N N 118.58 0.15 1 411 231 73 ASP H H 8.55 0.02 1 412 231 73 ASP HA H 4.65 0.02 1 413 231 73 ASP C C 176.35 0.20 1 414 231 73 ASP CA C 54.45 0.20 1 415 231 73 ASP CB C 41.07 0.20 1 416 231 73 ASP N N 121.12 0.15 1 417 232 74 SER H H 8.33 0.02 1 418 232 74 SER HA H 4.42 0.02 1 419 232 74 SER C C 174.38 0.20 1 420 232 74 SER CA C 58.62 0.20 1 421 232 74 SER CB C 63.75 0.20 1 422 232 74 SER N N 115.61 0.15 1 423 233 75 ASP H H 8.41 0.02 1 424 233 75 ASP HA H 4.63 0.02 1 425 233 75 ASP C C 175.97 0.20 1 426 233 75 ASP CA C 54.48 0.20 1 427 233 75 ASP CB C 40.88 0.20 1 428 233 75 ASP N N 122.02 0.15 1 429 234 76 ASN H H 8.28 0.02 1 430 234 76 ASN HA H 4.69 0.02 1 431 234 76 ASN HD21 H 7.68 0.02 1 432 234 76 ASN HD22 H 6.95 0.02 1 433 234 76 ASN C C 174.72 0.20 1 434 234 76 ASN CA C 53.22 0.20 1 435 234 76 ASN CB C 39.12 0.20 1 436 234 76 ASN N N 118.60 0.15 1 437 234 76 ASN ND2 N 113.18 0.15 1 438 235 77 ASP H H 8.38 0.02 1 439 235 77 ASP HA H 4.60 0.02 1 440 235 77 ASP C C 176.22 0.20 1 441 235 77 ASP CA C 54.27 0.20 1 442 235 77 ASP CB C 40.85 0.20 1 443 235 77 ASP N N 120.73 0.15 1 444 236 78 LEU H H 8.26 0.02 1 445 236 78 LEU HA H 4.35 0.02 1 446 236 78 LEU C C 177.45 0.20 1 447 236 78 LEU CA C 55.13 0.20 1 448 236 78 LEU CB C 42.08 0.20 1 449 236 78 LEU N N 122.86 0.15 1 450 237 79 SER H H 8.46 0.02 1 451 237 79 SER HA H 4.42 0.02 1 452 237 79 SER C C 174.55 0.20 1 453 237 79 SER CA C 58.38 0.20 1 454 237 79 SER CB C 63.52 0.20 1 455 237 79 SER N N 116.86 0.15 1 456 238 80 LEU H H 8.27 0.02 1 457 238 80 LEU HA H 4.36 0.02 1 458 238 80 LEU C C 177.29 0.20 1 459 238 80 LEU CA C 55.06 0.20 1 460 238 80 LEU CB C 42.16 0.20 1 461 238 80 LEU N N 123.93 0.15 1 462 239 81 GLU H H 8.27 0.02 1 463 239 81 GLU HA H 4.25 0.02 1 464 239 81 GLU C C 175.67 0.20 1 465 239 81 GLU CA C 56.23 0.20 1 466 239 81 GLU CB C 30.34 0.20 1 467 239 81 GLU N N 120.54 0.15 1 468 240 82 ASP H H 8.23 0.02 1 469 240 82 ASP HA H 4.59 0.02 1 470 240 82 ASP C C 174.59 0.20 1 471 240 82 ASP CA C 54.08 0.20 1 472 240 82 ASP CB C 41.15 0.20 1 473 240 82 ASP N N 121.33 0.15 1 474 241 83 PHE H H 7.64 0.02 1 475 241 83 PHE HA H 4.43 0.02 1 476 241 83 PHE CA C 58.90 0.20 1 477 241 83 PHE CB C 40.34 0.20 1 478 241 83 PHE N N 124.88 0.15 1 stop_ save_