BMRB Entry 6415

Name: Three-disulfide variant of hen lysozyme: C30A/C115A
Published: 2005-04-04

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6415

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Three-disulfide variant of hen lysozyme: C30A/C115A

Deposition date:

2004-12-07

Original release date:

2005-04-04

Authors:

Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi

Citation:

Citation: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR Characterization of Three-Disulfide Variants of Lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A -A Marginally Stable State in Folded Proteins" Biochemistry 43, 6663-6669 (2004).
PubMed: 15157100

Assembly members:

Assembly members:
hen egg white lysozyme variant, polymer, 130 residues, 14374 Da.

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hen egg white lysozyme variant: MKVFGRCELAAAMKRHGLDN YRGYSLGNWVAAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWCNDGRTPGSRNLCNIP CSALLSSDITASVNCAKKIV SDGNGMNAWVAWRNRAKGTD VQAWIRGCRL

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	752

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hen lysozyme	1

Entities:

Entity 1, hen lysozyme 130 residues - 14374 Da.

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	ALA	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	ALA	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

Samples:

sample_1: hen egg white lysozyme variant, [U-15N], 0.2 – 1.0 mM

EX-cond_1: pH: 3.8 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
DQF-COSY	sample_1	not available	EX-cond_1
NOESY	sample_1	not available	EX-cond_1
HOHAHA	sample_1	not available	EX-cond_1
15N 3D-NOESY-HSQC	sample_1	not available	EX-cond_1
15N 3D-TOCSY-HSQC	sample_1	not available	EX-cond_1
1H-15N HSQC	sample_1	not available	EX-cond_1
15N 3D-HSQC-NOESY-HSQC	sample_1	not available	EX-cond_1

Software:

XWINNMR v2.6 -

NMR spectrometers:

Bruker DRX 600 MHz

Related Database Links:

GenBank	AAL69327 AAA48943
SWISS-PROT	P00698
PDB	8LYZ 6LYZ 7LYZ 5LYZ 6LYT 5LYM 5LYT 4LYZ 4LZT 4LYO 4LYT 3LZT 4LYM 3LYT 3LYZ 3LYM 3LYO 3D9A 3HFM 2Z19 3B6L 2Z12 2Z18 2YSS 2YVB 2Q0M 2VB1 2LZT 2PC2 2LYZ 2LZH 2LYM 2LYO 2I26 2I6Z 2HUB 2I25 2HU1 2HU3 2HSO 2HTX 2HS7 2HS9 2H9J 2H9K 2G4P 2G4Q 2F4G 2FBB 2F30 2F4A 2EPE 2F2N 2EIZ 2EKS 2DQI 2DQJ 2DQG 2DQH 2DQE 2DQF 2DQC 2DQD 2D6B 2D91 2D4J 2D4K 2CGI 2D4I 2C8P 2CDS 2BPU 2C8O 2BLX 2BLY 2AUB 2B5Z 2A7D 2A7F 1ZVY 2A6U 1ZV5 1ZVH 1Z55 1ZMY 1YL1 1YQV 1YKZ 1YL0 1YKX 1YKY 1YIK 1YIL 1XGT 1XGU 1XGQ 1XGR 1XFP 1XGP 1XEJ 1XEK 1WTN 1XEI 1W6Z 1WTM 1VED 1VFB 1VDS 1VDT 1VDP 1VDQ 1VAT 1VAU 1V7S 1V7T 1UIH 1UUZ 1UCO 1UIG 1UA6 1UC0 1T3P 1T6V 1SFG 1SQ2 1SF7 1SFB 1SF4 1SF6 1RI8 1RJC 1RCM 1RFP 1QIO 1QTK 1P2C 1PS5 1N4F 1NDM 1MEL 1MLC 1LZN 1LZT 1LZC 1LZH 1LZA 1LZB 1LZ8 1LZ9 1LYS 1LYZ 1LSG 1LYO 1LSE 1LSF 1LSC 1LSD 1LSA 1LSB 1LMA 1LPI 1LKR 1LKS 1LJJ 1LJK 1LJH 1LJI 1LJF 1LJG 1LJ4 1LJE 1LCN 1LJ3 1KIQ 1KIR 1JTT 1KIP 1JPO 1JTO 1JJ1 1JJ3 1JIY 1JJ0 1JIS 1JIT 1JA6 1JA7 1JA2 1JA4 1J1P 1J1X 1IO5 1J1O 1IC7 1IEE 1IC4 1IC5 1HSW 1HSX 1HEW 1HF4 1HC0 1HEL 1GXX 1H87 1GWD 1GXV 1G7M 1GPQ 1G7J 1G7L 1G7H 1G7I 1F10 1FDL 1E8L 1F0W 1DPX 1DQJ 1C10 1DPW 1BWJ 1C08 1BWH 1BWI 1BVK 1BVX 1BGI 1BHZ 1B0D 1B2K 1AKI 1AZF 193L 194L
BMRB	4943 5068 1093 4562

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	ALA	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	ALA	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	ALA	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	ALA	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	ALA	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	ALA	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU