BMRB Entry 54052
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR54052
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NMR-STAR v3 text file.
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Citation: Shu, Chang. "Backbone and side-chain 1H, 13C, and 15N resonance assignments of the human Sin3A PAH2 domain (residues 295-395)
" Biomol. NMR Assignments ., .-..
Assembly members:
Sin3A-PAH2(295-395), polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a
Entity Sequences (FASTA):
Sin3A-PAH2(295-395): SLQNNQPVEFNHAINYVNKI
KNRFQGQPDIYKAFLEILHT
YQKEQRNAKEAGGNYTPALT
EQEVYAQVARLFKNQEDLLS
EFGQFLPDANSSVLLSKTTA
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 423 |
| 15N chemical shifts | 119 |
| 1H chemical shifts | 688 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sin3A-PAH2(295-395) | 1 |
Entities:
Entity 1, Sin3A-PAH2(295-395) 101 residues - Formula weight is not available
| 1 | SER | LEU | GLN | ASN | ASN | GLN | PRO | VAL | GLU | PHE | ||||
| 2 | ASN | HIS | ALA | ILE | ASN | TYR | VAL | ASN | LYS | ILE | ||||
| 3 | LYS | ASN | ARG | PHE | GLN | GLY | GLN | PRO | ASP | ILE | ||||
| 4 | TYR | LYS | ALA | PHE | LEU | GLU | ILE | LEU | HIS | THR | ||||
| 5 | TYR | GLN | LYS | GLU | GLN | ARG | ASN | ALA | LYS | GLU | ||||
| 6 | ALA | GLY | GLY | ASN | TYR | THR | PRO | ALA | LEU | THR | ||||
| 7 | GLU | GLN | GLU | VAL | TYR | ALA | GLN | VAL | ALA | ARG | ||||
| 8 | LEU | PHE | LYS | ASN | GLN | GLU | ASP | LEU | LEU | SER | ||||
| 9 | GLU | PHE | GLY | GLN | PHE | LEU | PRO | ASP | ALA | ASN | ||||
| 10 | SER | SER | VAL | LEU | LEU | SER | LYS | THR | THR | ALA | ||||
| 11 | GLU |
Samples:
sample_1: Sin3A-PAH2(295-395), [U-100% 13C; U-100% 15N], 0.5 mM; PBS 20 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298K K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks