Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53883

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H and 15N chemical shift assignments for yeast Rub1

Deposition date:

2026-06-24

Original release date:

2026-07-01

Authors:

Jou, Jane; Singh, Rajesh; Lemma, Betsegaw; Fushman, David

Citation:

Citation: Greaves, I; Colebatch, H. "Observations on the pathogenesis of chronic airflow obstruction in smokers: implications for the detection of "early" lung disease. " Thorax 41, 81-87 (1986).
PubMed: 3518131

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, 8579.06 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTXB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MIVKVKTLTGKEISVELKES DLVYHIKELLEEKEGIPPSQ QRLIFQGKQIDDKLTVTDAH LVEGMQLHLVLTLRGG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rub1	1

Entities:

Entity 1, Rub1 76 residues - 8579.06 Da.

1

MET

ILE

VAL

LYS

VAL

LYS

THR

LEU

THR

GLY

2

LYS

GLU

ILE

SER

VAL

GLU

LEU

LYS

GLU

SER

3

ASP

LEU

VAL

TYR

HIS

ILE

LYS

GLU

LEU

4

GLU

LYS

GLU

GLY

ILE

PRO

SER

GLN

5

GLN

ARG

LEU

ILE

PHE

GLN

GLY

LYS

GLN

ILE

6

ASP

LYS

LEU

THR

VAL

THR

ASP

ALA

HIS

7

LEU

VAL

GLU

GLY

MET

GLN

LEU

HIS

LEU

VAL

8

LEU

THR

LEU

ARG

GLY

Samples:

sample_1: Rub1, [U-100% 15N], 250 uM; sodium phosphate 20 mM; sodium azide 0.02 % (v/v)

sample_2: Rub1 250 uM; sodium phosphate 20 mM; sodium azide 0.02 % (v/v)

sample_3: Rub1, [U-100% 15N/13C], 250 uM; sodium phosphate 20 mM; sodium azide 0.02 % (v/v)

sample_conditions_1: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1
3D 15N-separated TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
3D CBCACONH	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCACO	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HN(CO)CA	sample_3	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY v1.470 - chemical shift assignment, peak picking

TOPSPIN v4.5 - data collection, data processing

NMR spectrometers:

Bruker AVANCE III 600 MHz

BMRB Entry 53883