BMRB Entry 53838
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53838
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Hu, Rong; Usher, Emery; Fraser, Olivia; Chen, Wei; Holehouse, Alex; Showalter, Scott. "Multisite Threonine Phosphorylation in the SPT5 C-Terminal Region 1 Enables Sequence-Dependent Regulation without Global Structural Remodeling
" J. Phys. Chem. B 130, 8409-8422 (2026).
PubMed: 42622275
Assembly members:
entity_1, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 MBP TEV LIC
Entity Sequences (FASTA):
entity_1: SNAGSVSTYGRTPARTPGYG
AQTPSYTAAGSKTPLVGSQT
PNWDTDTRTPYGTMTPSHDG
SMTPRHGAWDPTANTTPARN
NDFDYSLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 298 |
| 15N chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SPT5-CTR1 | 1 |
Entities:
Entity 1, SPT5-CTR1 88 residues - Formula weight is not available
| 1 | SER | ASN | ALA | GLY | SER | VAL | SER | THR | TYR | GLY | ||||
| 2 | ARG | THR | PRO | ALA | ARG | THR | PRO | GLY | TYR | GLY | ||||
| 3 | ALA | GLN | THR | PRO | SER | TYR | THR | ALA | ALA | GLY | ||||
| 4 | SER | LYS | THR | PRO | LEU | VAL | GLY | SER | GLN | THR | ||||
| 5 | PRO | ASN | TRP | ASP | THR | ASP | THR | ARG | THR | PRO | ||||
| 6 | TYR | GLY | THR | MET | THR | PRO | SER | HIS | ASP | GLY | ||||
| 7 | SER | MET | THR | PRO | ARG | HIS | GLY | ALA | TRP | ASP | ||||
| 8 | PRO | THR | ALA | ASN | THR | THR | PRO | ALA | ARG | ASN | ||||
| 9 | ASN | ASP | PHE | ASP | TYR | SER | LEU | GLU |
Samples:
sample_1: SPT5-CTR1, [U-100% 13C; U-100% 15N], 800 uM; H2O 95%; D2O 5%; imidazole 50 mM; potassium chloride 200 mM
sample_conditions_1: ionic strength: 450 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 15N-13C CON | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N-13C-Proline-CON | sample_1 | isotropic | sample_conditions_1 |
| 3D (HACA)N(CA)CON | sample_1 | isotropic | sample_conditions_1 |
| 3D (HACA)N(CA)NCO | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCCON | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCCdNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D Proline-selective (H)CCCON | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2.6 - collection, processing
POKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz