BMRB Entry 53608

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53608

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
1H, 13C and 15N assignment of the flexible segments of WT alpha-synuclein bound to lipid vesicles comprised of 1,2-dioleoyl-sn-glycero-3-phosphate/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPA/DOPC) with varying negative charge (25%, 35%, 40%)
Deposition date:
2026-03-09
Original release date:
2026-09-14
Authors:
Lavasani, Mahdi; Lu, Ping; Siripala, Shehani; Medeiros, Justin; Coackley, Carla; Ryan, Scott; Ladizhansky, Vladimir
Citation:

Citation: Lavasani, Mahdi; Lu, Ping; Siripala, Shehani; Medeiros, Justin; Coackley, Carla; Ryan, Scott; Ladizhansky, Vladimir. "Electrostatic Regulation of Alpha-Synuclein Membrane Binding Couples Conformational Release to Aggregation Kinetics "  J. Am. Chem. Soc. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data typeCount
13C chemical shifts502
15N chemical shifts179
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-synuclein1

Entities:

Entity 1, alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: alpha-synuclein, [U-99% 13C; U-99% 15N], 100 uM; potassium phosphate 10 mM; DOPA:DOPC (25:75) 10 mM; H2O 100%

sample_2: alpha-synuclein, [U-99% 13C; U-99% 15N], 100 uM; potassium phosphate 10 mM; DOPA:DOPC (35:65) 10 mM; H2O 100%

sample_3: alpha-synuclein, [U-99% 13C; U-99% 15N], 100 uM; potassium phosphate 10 mM; DOPA:DOPC (40:60) 10 mM; H2O 100%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 281 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CcpNMR v3.3.4 - chemical shift assignment

NMRPipe - processing

TOPSPIN v3.6.5 - collection

NMR spectrometers:

  • Bruker AVANCE III 800.230 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks