BMRB Entry 53120
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53120
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Lai, Chih-Hsuan; Lou, Yuan-Chao; Chang, Chi-Fon; Lu, Wei-Lin; Sriramoju, Manoj Kumar; Wang, Yong-Sheng; Wu, Kuen-Phon; Camilloni, Carlo; Hsu, Shang-Te Danny. "Allosteric network of dynamic coupling within BAP1-UCH revealed by methyl NMR
" Nat. Commun. 17, 6867-6867 (2026).
PubMed: 42191708
Assembly members:
BAP1-UCH_WT, polymer, 238 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pET28a
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 72 |
| 1H chemical shifts | 216 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BAP1-UCH | 1 |
Entities:
Entity 1, BAP1-UCH 238 residues - Formula weight is not available
| 1 | MET | ASN | LYS | GLY | TRP | LEU | GLU | LEU | GLU | SER | ||||
| 2 | ASP | PRO | GLY | LEU | PHE | THR | LEU | LEU | VAL | GLU | ||||
| 3 | ASP | PHE | GLY | VAL | LYS | GLY | VAL | GLN | VAL | GLU | ||||
| 4 | GLU | ILE | TYR | ASP | LEU | GLN | SER | LYS | CYS | GLN | ||||
| 5 | GLY | PRO | VAL | TYR | GLY | PHE | ILE | PHE | LEU | PHE | ||||
| 6 | LYS | TRP | ILE | GLU | GLU | ARG | ARG | SER | ARG | ARG | ||||
| 7 | LYS | VAL | SER | THR | LEU | VAL | ASP | ASP | THR | SER | ||||
| 8 | VAL | ILE | ASP | ASP | ASP | ILE | VAL | ASN | ASN | MET | ||||
| 9 | PHE | PHE | ALA | HIS | GLN | LEU | ILE | PRO | ASN | SER | ||||
| 10 | CYS | ALA | THR | HIS | ALA | LEU | LEU | SER | VAL | LEU | ||||
| 11 | LEU | ASN | CYS | SER | SER | VAL | ASP | LEU | GLY | PRO | ||||
| 12 | THR | LEU | SER | ARG | MET | LYS | ASP | PHE | THR | LYS | ||||
| 13 | GLY | PHE | SER | PRO | GLU | SER | LYS | GLY | TYR | ALA | ||||
| 14 | ILE | GLY | ASN | ALA | PRO | GLU | LEU | ALA | LYS | ALA | ||||
| 15 | HIS | ASN | SER | HIS | ALA | ARG | PRO | GLU | PRO | ARG | ||||
| 16 | HIS | LEU | PRO | GLU | LYS | GLN | ASN | GLY | LEU | SER | ||||
| 17 | ALA | VAL | ARG | THR | MET | GLU | ALA | PHE | HIS | PHE | ||||
| 18 | VAL | SER | TYR | VAL | PRO | ILE | THR | GLY | ARG | LEU | ||||
| 19 | PHE | GLU | LEU | ASP | GLY | LEU | LYS | VAL | TYR | PRO | ||||
| 20 | ILE | ASP | HIS | GLY | PRO | TRP | GLY | GLU | ASP | GLU | ||||
| 21 | GLU | TRP | THR | ASP | LYS | ALA | ARG | ARG | VAL | ILE | ||||
| 22 | MET | GLU | ARG | ILE | GLY | LEU | ALA | THR | ALA | GLY | ||||
| 23 | GLU | PRO | TYR | HIS | ASP | ILE | ARG | PHE | ASN | LEU | ||||
| 24 | MET | ALA | VAL | VAL | PRO | ASP | ARG | ARG |
Samples:
sample_1: BAP1-UCH_WT, [U-2H, 13Cm/1Hm-Ala-b, Ile-d1, Leu-d1/d2, Val-g1/g2], 120 uM; H2O 90%; D2O, [U-2H], 10%
sample_2: BAP1-UCH_WT, [U-2H, 13Cm/1Hm-Ala-b, Ile-d1, Leu-d2, Val-g2], 153 uM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
POKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 850 MHz