BMRB Entry 52867

Name: Eps15 IDR 761-896
Published: 2025-08-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52867

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Eps15 IDR 761-896

Deposition date:

2025-01-17

Original release date:

2025-08-18

Authors:

Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid

Citation:

Citation: Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid. "Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network" Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSVKSEDEPPALPPKIGTPT RPCPLPPGKRSINKLDSPDP FKLNDPFQPFPGNDSPKEKD PEIFCDPFTSATTTTNKEAD PSNFANFSAYPSEEDMIEWA KRESEREEEQRLARLNQQEQ EDLELAIALSKSEISEARLE HHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	334
15N chemical shifts	108
1H chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Eps15 IDR 761-896	1

Entities:

Entity 1, Eps15 IDR 761-896 148 residues - Formula weight is not available

1

MET

SER

VAL

LYS

SER

GLU

ASP

GLU

PRO

2

ALA

LEU

PRO

LYS

ILE

GLY

THR

PRO

THR

3

ARG

PRO

CYS

PRO

LEU

PRO

GLY

LYS

ARG

4

SER

ILE

ASN

LYS

LEU

ASP

SER

PRO

ASP

PRO

5

PHE

LYS

LEU

ASN

ASP

PRO

PHE

GLN

PRO

PHE

6

PRO

GLY

ASN

ASP

SER

PRO

LYS

GLU

LYS

ASP

7

PRO

GLU

ILE

PHE

CYS

ASP

PRO

PHE

THR

SER

8

ALA

THR

ASN

LYS

GLU

ALA

ASP

9

PRO

SER

ASN

PHE

ALA

ASN

PHE

SER

ALA

TYR

10

PRO

SER

GLU

ASP

MET

ILE

GLU

TRP

ALA

11

LYS

ARG

GLU

SER

GLU

ARG

GLU

GLN

12

ARG

LEU

ALA

ARG

LEU

ASN

GLN

GLU

GLN

13

GLU

ASP

LEU

GLU

LEU

ALA

ILE

ALA

LEU

SER

14

LYS

SER

GLU

ILE

SER

GLU

ALA

ARG

LEU

GLU

15

HIS

Samples:

sample_1: Eps15 IDR 761-896, [U-99% 13C; U-99% 15N], 500 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 211.6 mM; pH: 6; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks