BMRB Entry 52862
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52862
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NMR-STAR v3 text file.
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Citation: Wu, Shaowen; Yan, Shijuan. "Conformational Plasticity of Disordered Regions Enables Sequence-Diverse DNA Recognition by Transcription Factor AflR" Nat. Commun. ., .-..
Assembly members:
entity_1, polymer, 65 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Aspergillus flavus Taxonomy ID: 5059 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Aspergillus flavus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET.M.3C
Entity Sequences (FASTA):
entity_1: RSSQTRRARKLRDSCTSCAS
SKVRCTKEKPACARCIERGL
ACQYMVSKRMGRNPRAPSPL
DSTRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 176 |
| 15N chemical shifts | 60 |
| 1H chemical shifts | 61 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AflR-DBD | 1 |
| 2 | Zn ion, 1 | 2 |
| 3 | Zn ion, 2 | 2 |
Entities:
Entity 1, AflR-DBD 65 residues - Formula weight is not available
| 1 | ARG | SER | SER | GLN | THR | ARG | ARG | ALA | ARG | LYS | ||||
| 2 | LEU | ARG | ASP | SER | CYS | THR | SER | CYS | ALA | SER | ||||
| 3 | SER | LYS | VAL | ARG | CYS | THR | LYS | GLU | LYS | PRO | ||||
| 4 | ALA | CYS | ALA | ARG | CYS | ILE | GLU | ARG | GLY | LEU | ||||
| 5 | ALA | CYS | GLN | TYR | MET | VAL | SER | LYS | ARG | MET | ||||
| 6 | GLY | ARG | ASN | PRO | ARG | ALA | PRO | SER | PRO | LEU | ||||
| 7 | ASP | SER | THR | ARG | ARG |
Entity 2, Zn ion, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: AflR-DBD, [U-100% 13C; U-100% 15N], 0.6 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks