Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52084
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Citation: Yang, Min June; Park, Chin-Ju. "Backbone assignment of the intrinsically disordered N-terminal region of Bloom syndrome protein" J. Korean Mag. Res. Soc. 27, 17-22 (2023).
Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His 6 GST LIC
Entity Sequences (FASTA):
entity_1: SNALTEEQKDDSEWLSSDVI
CIDDGPIAEVHINEDAQESD
SLKTHLEDERDNSEKKKNLE
EAELHSTEKVPCIEFDDDDY
DTDF
Data type | Count |
13C chemical shifts | 242 |
15N chemical shifts | 72 |
1H chemical shifts | 72 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | BLM 220-300 | 1 |
Entity 1, BLM 220-300 84 residues - Formula weight is not available
1 | SER | ASN | ALA | LEU | THR | GLU | GLU | GLN | LYS | ASP | ||||
2 | ASP | SER | GLU | TRP | LEU | SER | SER | ASP | VAL | ILE | ||||
3 | CYS | ILE | ASP | ASP | GLY | PRO | ILE | ALA | GLU | VAL | ||||
4 | HIS | ILE | ASN | GLU | ASP | ALA | GLN | GLU | SER | ASP | ||||
5 | SER | LEU | LYS | THR | HIS | LEU | GLU | ASP | GLU | ARG | ||||
6 | ASP | ASN | SER | GLU | LYS | LYS | LYS | ASN | LEU | GLU | ||||
7 | GLU | ALA | GLU | LEU | HIS | SER | THR | GLU | LYS | VAL | ||||
8 | PRO | CYS | ILE | GLU | PHE | ASP | ASP | ASP | ASP | TYR | ||||
9 | ASP | THR | ASP | PHE |
sample_1: BLM 220-300, [U-100% 13C; U-100% 15N], 500 uM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
PINE - chemical shift assignment
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks