BMRB Entry 5122

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PDB ID: 1jwe
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5122
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp. PCC6803 DnaB Mini-intein

Deposition date:

2001-08-23

Original release date:

2002-04-08

Authors:

Williams, Neal; Prosselkov, Pavel; Liepinsh, Edvards; Line, Inara; Sharipo, Anatoly; Littler, Dene; Curmi, Paul; Otting, Gottfried; Dixon, Nicholas

Citation:

Citation: Williams, Neal; Prosselkov, Pavel; Liepinsh, Edvards; Line, Inara; Sharipo, Anatoly; Littler, Dene; Curmi, Paul; Otting, Gottfried; Dixon, Nicholas. "In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp. PCC6803 DnaB Mini-intein" J. Biol. Chem. 277, 7790-7798 (2002).
PubMed: 11742000

Assembly members:

Assembly members:
N-terminal Domain of E. coli DnaB Helicase, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal Domain of E. coli DnaB Helicase: KVPPHSIEAEQSVLGGLMLD NERWDDVAERVVADDFYTRP HRHIFTEMARLQESGSPIDL ITLAESLERQGQLDSVGGFA YLAELSKNTPSAANISAYAD IVRERAVVREMISTRESGSI EF

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
15N chemical shifts	122
1H chemical shifts	835

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cz-DnaB-N(24-136)	1

Entities:

Entity 1, cz-DnaB-N(24-136) 122 residues - Formula weight is not available

1

LYS

VAL

PRO

HIS

SER

ILE

GLU

ALA

GLU

2

GLN

SER

VAL

LEU

GLY

LEU

MET

LEU

ASP

3

ASN

GLU

ARG

TRP

ASP

VAL

ALA

GLU

ARG

4

VAL

ALA

ASP

PHE

TYR

THR

ARG

PRO

5

HIS

ARG

HIS

ILE

PHE

THR

GLU

MET

ALA

ARG

6

LEU

GLN

GLU

SER

GLY

SER

PRO

ILE

ASP

LEU

7

ILE

THR

LEU

ALA

GLU

SER

LEU

GLU

ARG

GLN

8

GLY

GLN

LEU

ASP

SER

VAL

GLY

PHE

ALA

9

TYR

LEU

ALA

GLU

LEU

SER

LYS

ASN

THR

PRO

10

SER

ALA

ASN

ILE

SER

ALA

TYR

ALA

ASP

11

ILE

VAL

ARG

GLU

ARG

ALA

VAL

ARG

GLU

12

MET

ILE

SER

THR

ARG

GLU

SER

GLY

SER

ILE

13

GLU

PHE

Samples:

sample_1: N-terminal Domain of E. coli DnaB Helicase 2.1 mM

sample_2: N-terminal Domain of E. coli DnaB Helicase, [U-95% 15N], 2.1 mM

ex-cond_1: ionic strength: 0.1 M; pH*: 7.0; temperature: 304 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N TOCSY	not available	not available	not available
NOESY	not available	not available	not available
TOCSY	not available	not available	not available
COSY	not available	not available	not available

Software:

Prosa v3.6 - spectra processing

XEASY v1.2 - peak analysis

NMR spectrometers:

Varian INOVA 800 MHz
Bruker DMX 600 MHz
Bruker DMX 500 MHz

BMRB	4075 4297 5121
PDB	1B79 1JWE
DBJ	BAB38457 BAE78054 BAG66812
GenBank	AAA23689 AAA23690 AAC43146 AAC77022 AAG59250
REF	AP_004553 NP_290685 NP_313061 NP_418476 NP_709868
SWISS-PROT	P0ACB0 P0ACB1 Q8FB22 Q8X5V3