BMRB Entry 4785

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4785

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N assignment of a bleomycin resistance protein in its native form and in a complex with Zn(2+) ligated bleomycin

Deposition date:

2000-07-17

Original release date:

2000-10-30

Authors:

Vanbelle, Christophe; Muhle-Goll, Claudia; Remy, Marie-Helene; Masson, Jean-Michel; Marion, Dominique; Brutscher, Bernhard

Citation:

Citation: Vanbelle, Christophe; Muhle-Goll, Claudia; Remy, Marie-Helene; Masson, Jean-Michel; Marion, Dominique; Brutscher, Bernhard. "Letter to the Editor: 1H, 13C, and 15N assignment of a bleomycin resistance protein in its native form and in a complex with Zn2+ ligated bleomycin" J. Biomol. NMR 18, 177-178 (2000).

Assembly members:

Assembly members:
S. hindustanus bleomycin resistance protein, polymer, 124 residues, 13796 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 2017 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptoalloteichus hindustanus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S. hindustanus bleomycin resistance protein: MAKLTSAVPVLTARDVAGAV EFWTDRLGFSRDFVEDDFAG VVRDDVTLFISAVQDQVVPD NTLAWVWVRGLDELYAEWSE VVSTNFRDASGPAMTEIGEQ PWGREFALRDPAGNCVHFVA EEQD

Data sets:

assigned_chemical_shifts
- Nat_Shift

Data type	Count
1H chemical shifts	115
13C chemical shifts	228
15N chemical shifts	115

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BRP monomer 1	1
2	BRP monomer 2	1

Entities:

Entity 1, BRP monomer 1 124 residues - 13796 Da.

1

MET

ALA

LYS

LEU

THR

SER

ALA

VAL

PRO

VAL

2

LEU

THR

ALA

ARG

ASP

VAL

ALA

GLY

ALA

VAL

3

GLU

PHE

TRP

THR

ASP

ARG

LEU

GLY

PHE

SER

4

ARG

ASP

PHE

VAL

GLU

ASP

PHE

ALA

GLY

5

VAL

ARG

ASP

VAL

THR

LEU

PHE

ILE

6

SER

ALA

VAL

GLN

ASP

GLN

VAL

PRO

ASP

7

ASN

THR

LEU

ALA

TRP

VAL

TRP

VAL

ARG

GLY

8

LEU

ASP

GLU

LEU

TYR

ALA

GLU

TRP

SER

GLU

9

VAL

SER

THR

ASN

PHE

ARG

ASP

ALA

SER

10

GLY

PRO

ALA

MET

THR

GLU

ILE

GLY

GLU

GLN

11

PRO

TRP

GLY

ARG

GLU

PHE

ALA

LEU

ARG

ASP

12

PRO

ALA

GLY

ASN

CYS

VAL

HIS

PHE

VAL

ALA

13

GLU

GLN

ASP

Samples:

sample_1: S. hindustanus bleomycin resistance protein, [U-99% 15N], 1.0 mM

sample_2: S. hindustanus bleomycin resistance protein, [U-98% 13C; U-99% 15N], 1 mM

Cond_1: pH*: 6.5; temperature: 313 K; ionic strength: 0.1 M

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

FELIX v97 -

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz

BMRB	4786
PDB	1BYL 2ZHP
DBJ	BAC41730 BAF75469 BAG54978 BAG71041 BAI39424
EMBL	CAA37050 CAA83658 CAD21454
GB	AAA72449 AAA72451 AAA73162 AAA73163 AAA93507
SP	P17493
AlphaFold	P17493