BMRB Entry 4769

Name: Backbone 1H, 13C and 15N assignments for yeast ubiquitin at pH 7.5
Published: 2001-05-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4769

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N assignments for yeast ubiquitin at pH 7.5

Deposition date:

2000-06-22

Original release date:

2001-05-03

Authors:

Hamilton, Katherine; Ellison, Michael; Shaw, Gary

Citation:

Citation: Hamilton, Katherine; Ellison, Michael; Shaw, Gary. "Identification of the Ubiquitin Interfacial Residues in a Ubiquitin-E2 Covalent Complex" J. Biomol. NMR 18, 319-327 (2000).
PubMed: 11200526

Assembly members:

Assembly members:
yeast ubiquitin, polymer, 76 residues, 8500 Da.

Natural source:

Natural source: Common Name: yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yeast ubiquitin: MQIFVKTLTGKTITLEVESS DTIDNVKSKIQDKEGIPPDQ QRLIFAGRQLEDGRTLSDYN IQKESTLHLVLRLRGG

Data sets:

assigned_chemical_shifts
- chemical_shift_set1

Data type	Count
13C chemical shifts	133
15N chemical shifts	76
1H chemical shifts	321

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yeast ubiquitin	1

Entities:

Entity 1, yeast ubiquitin 76 residues - 8500 Da.

1

MET

GLN

ILE

PHE

VAL

LYS

THR

LEU

THR

GLY

2

LYS

THR

ILE

THR

LEU

GLU

VAL

GLU

SER

3

ASP

THR

ILE

ASP

ASN

VAL

LYS

SER

LYS

ILE

4

GLN

ASP

LYS

GLU

GLY

ILE

PRO

ASP

GLN

5

GLN

ARG

LEU

ILE

PHE

ALA

GLY

ARG

GLN

LEU

6

GLU

ASP

GLY

ARG

THR

LEU

SER

ASP

TYR

ASN

7

ILE

GLN

LYS

GLU

SER

THR

LEU

HIS

LEU

VAL

8

LEU

ARG

LEU

ARG

GLY

Samples:

sample_1: yeast ubiquitin, [U-95% 13C; U-90% 15N], 0.8 mM; HEPES 40 mM; NaCl 50 mM; EDTA 1 mM

condition1: pH: 7.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HNCACB	not available	not available	not available
1H-15N HSQC	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian Unity 500 MHz

Related Database Links:

BMRB	15592 17024 4983
PDB	1OTR 1Q0W 1VW8 1VW9 1VWU 1VWV 1VXV 1VXW 1VXY 1VXZ 1WR1 2G3Q 2JT4 2JWZ 2L00 3CMM 3J80 3J81 3L0W 3L10 3OLM 3U5C 3U5E 3U5G 3U5I 4B6A 4BYL 4BYN 4BYT 4BYU 4CUW 4HCN 4II3 4NNJ 4Q5E 4Q5H
DBJ	BAA76889 BAA88168 BAB79488 BAD97865 BAE57827
EMBL	CAA07773 CAA11267 CAA21278 CAA25705 CAA25706
GB	AAA03351 AAA36787 AAA42855 AAA56880 AAA72565
PRF	1101405A
REF	NP_012118 NP_013020 NP_013061 NP_013268 NP_593923
SP	P05759 P0C016 P0C224 P0C8R3 P0CG63
TPD	FAA00316 FAA00317 FAA00318 FAA00319
TPE	CBF76581 CBF85986
TPG	DAA08405 DAA09244 DAA09283 DAA09489
AlphaFold	P05759 P0C016 P0C224 P0C8R3 P0CG63

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks