BMRB Entry 4337

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4337

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate-Containing H-protein of the Glycine Decarboxylase Complex.

Deposition date:

1999-04-23

Original release date:

1999-05-10

Authors:

Guilhaudis, Laure; Simorre, Jean-Pierre; Bouchayer, Eftychia; Neuburger, Michel; Bourguignon, Jacques; Douce, Roland; Marion, Dominique; Gans, Pierre

Citation:

Citation: Guilhaudis, Laure; Simorre, Jean-Pierre; Bouchayer, Eftychia; Neuburger, Michel; Bourguignon, Jacques; Douce, Roland; Marion, Dominique; Gans, Pierre. "Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate-Containing H-protein of the Glycine Decarboxylase Complex." J. Biomol. NMR 15, 185-186 (1999).
PubMed: 10605092

Assembly members:

Assembly members:
glycine cleavage system H-protein, polymer, 131 residues, 13948 Da.
LPA, non-polymer, 206.326 Da.

Natural source:

Natural source: Common Name: Garden Pea Taxonomy ID: 3888 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pisum sativum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia Coli Vector: pET-H

Entity Sequences (FASTA):

Entity Sequences (FASTA):
glycine cleavage system H-protein: SNVLDGLKYAPSHEWVKHEG SVATIGITDHAQDHLGEVVF VELPEPGVSVTKGKGFGAVE SVKATSDVNSPISGEVIEVN TGLTGKPGLINSSPYEDGWM IKIKPTSPDELESLLGAKEY TKFCEEEDAAH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
15N chemical shifts	119
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hox subunit 1	1
2	lipoate	2

Entities:

Entity 1, Hox subunit 1 131 residues - 13948 Da.

1

SER

ASN

VAL

LEU

ASP

GLY

LEU

LYS

TYR

ALA

2

PRO

SER

HIS

GLU

TRP

VAL

LYS

HIS

GLU

GLY

3

SER

VAL

ALA

THR

ILE

GLY

ILE

THR

ASP

HIS

4

ALA

GLN

ASP

HIS

LEU

GLY

GLU

VAL

PHE

5

VAL

GLU

LEU

PRO

GLU

PRO

GLY

VAL

SER

VAL

6

THR

LYS

GLY

LYS

GLY

PHE

GLY

ALA

VAL

GLU

7

SER

VAL

LYS

ALA

THR

SER

ASP

VAL

ASN

SER

8

PRO

ILE

SER

GLY

GLU

VAL

ILE

GLU

VAL

ASN

9

THR

GLY

LEU

THR

GLY

LYS

PRO

GLY

LEU

ILE

10

ASN

SER

PRO

TYR

GLU

ASP

GLY

TRP

MET

11

ILE

LYS

ILE

LYS

PRO

THR

SER

PRO

ASP

GLU

12

LEU

GLU

SER

LEU

GLY

ALA

LYS

GLU

TYR

13

THR

LYS

PHE

CYS

GLU

ASP

ALA

14

HIS

Entity 2, lipoate - C8 H14 O2 S2 - 206.326 Da.

1

LPA

Samples:

sample_one: glycine cleavage system H-protein, [U-99% 15N], 1.3 mM; potassium phosphate 50 mM; EDTA 0.1 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_conditions_one: pH: 5.5 na; temperature: 291 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY-HSQC	sample_one	not available	sample_conditions_one
3D 1H-15N TOCSY-HSQC	sample_one	not available	sample_conditions_one
2D 1H-15N HSQC	sample_one	not available	sample_conditions_one

Software:

FELIX v95.0, 97.0 -

NMR spectrometers:

Bruker AMX 600 MHz

BMRB	4336 4338
PDB	1DXM 1HPC 1HTP
EMBL	CAA37704 CAA45978 CAI79404 CAJ13415 CAJ13721
GB	AAA33668
PRF	1923203A
SP	P16048
AlphaFold	P16048