BMRB Entry 4291
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4291
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Citation: Messias, A.; Kastrau, D.; Costa, H.; Legall, J.; Turner, D.; Santos, H.; Xavier, A.. "Solution Structure of Desulfovibrio Vulgaris (Hildenborough)
Ferrocytochrome C3: Structural Basis for Functional Cooperativity" J. Mol. Biol. 281, 719-739 (1998).
Assembly members:
Cytochrome C3, polymer, 107 residues, 14000 Da.
HEME C, non-polymer, 618.503 Da.
Natural source: Common Name: Desulfovibrio vulgaris Taxonomy ID: 882 Superkingdom: Eubacteria Kingdom: not available Genus/species: Desulfovibrio vulgaris
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Cytochrome C3: APKAPADGLKMEATKQPVVF
NHSTHKSVKCGDCHHPVNGK
EDYRKCGTAGCHDSMDKKDK
SAKGYYHVMHDKNTKFKSCV
GCHVEVAGADAAKKKDLTGC
KKSKCHE
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 668 |
| coupling constants | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome_c3_peptide | 1 |
| 2 | heme_I | 2 |
| 3 | heme_II | 2 |
| 4 | heme_III | 2 |
| 5 | heme_IV | 2 |
Entities:
Entity 1, cytochrome_c3_peptide 107 residues - 14000 Da.
| 1 | ALA | PRO | LYS | ALA | PRO | ALA | ASP | GLY | LEU | LYS | ||||
| 2 | MET | GLU | ALA | THR | LYS | GLN | PRO | VAL | VAL | PHE | ||||
| 3 | ASN | HIS | SER | THR | HIS | LYS | SER | VAL | LYS | CYS | ||||
| 4 | GLY | ASP | CYS | HIS | HIS | PRO | VAL | ASN | GLY | LYS | ||||
| 5 | GLU | ASP | TYR | ARG | LYS | CYS | GLY | THR | ALA | GLY | ||||
| 6 | CYS | HIS | ASP | SER | MET | ASP | LYS | LYS | ASP | LYS | ||||
| 7 | SER | ALA | LYS | GLY | TYR | TYR | HIS | VAL | MET | HIS | ||||
| 8 | ASP | LYS | ASN | THR | LYS | PHE | LYS | SER | CYS | VAL | ||||
| 9 | GLY | CYS | HIS | VAL | GLU | VAL | ALA | GLY | ALA | ASP | ||||
| 10 | ALA | ALA | LYS | LYS | LYS | ASP | LEU | THR | GLY | CYS | ||||
| 11 | LYS | LYS | SER | LYS | CYS | HIS | GLU |
Entity 2, heme_I - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_one: Cytochrome C3 2 mM; ampicillin 70 uM; kanamicin 50 uM; chloramphenicol 50 uM
sample_conditions_one: pH: 8.5 na; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-1H-NOESY | sample_one | not available | sample_conditions_one |
| 2D-1H-TOCSY | sample_one | not available | sample_conditions_one |
| 2D-1H-COSY | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz