BMRB Entry 19203

Name: NMR structure of RNA recognition motif 2 (RRM2) of Homo sapiens splicing factor, arginine/serine-rich 1
Published: 2013-05-13

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PDB ID: 2m7s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19203
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of RNA recognition motif 2 (RRM2) of Homo sapiens splicing factor, arginine/serine-rich 1

Deposition date:

2013-04-29

Original release date:

2013-05-13

Authors:

Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt

Citation:

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of RNA recognition motif 2 (RRM2) of Homo sapiens splicing factor, arginine/serine-rich 1" .

Assembly members:

Assembly members:
RRM2, polymer, 90 residues, 10207.483 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: SpeedET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM2: GAPRGRYGPPSRRSENRVVV SGLPPSGSWQDLKDHMREAG DVCYADVYRDGTGVVEFVRK EDMTYAVRKLDNTKFRSHEG ETAYIRVKVD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	281
15N chemical shifts	87
1H chemical shifts	593

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM2 of Homo sapiens splicing factor, arginine/serine-rich 1	1

Entities:

Entity 1, RRM2 of Homo sapiens splicing factor, arginine/serine-rich 1 90 residues - 10207.483 Da.

1	GLY	ALA	PRO	ARG	GLY	ARG	TYR	GLY	PRO	PRO
2	SER	ARG	ARG	SER	GLU	ASN	ARG	VAL	VAL	VAL
3	SER	GLY	LEU	PRO	PRO	SER	GLY	SER	TRP	GLN
4	ASP	LEU	LYS	ASP	HIS	MET	ARG	GLU	ALA	GLY
5	ASP	VAL	CYS	TYR	ALA	ASP	VAL	TYR	ARG	ASP
6	GLY	THR	GLY	VAL	VAL	GLU	PHE	VAL	ARG	LYS
7	GLU	ASP	MET	THR	TYR	ALA	VAL	ARG	LYS	LEU
8	ASP	ASN	THR	LYS	PHE	ARG	SER	HIS	GLU	GLY
9	GLU	THR	ALA	TYR	ILE	ARG	VAL	LYS	VAL	ASP

Samples:

sample_1: RRM2, [U-99% 13C; U-99% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.0798 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
4D-HACANH-APSY	sample_1	isotropic	sample_conditions_1
5D-CBCACONH-APSY	sample_1	isotropic	sample_conditions_1
5D-HACACONH-APSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic with non-uniform sampling	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic with non-uniform sampling	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY with non-uniform sampling	sample_1	isotropic	sample_conditions_1

Software:

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

j-UNIO, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking, structure solution

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

TOPSPIN v3.1, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

BMRB	19248
PDB	1X4C 2M7S 2M8D 2O3D 3BEG 4C0O
DBJ	BAB93456 BAC37367 BAE29641 BAE88160 BAF82622
EMBL	CAH92288
GB	AAA03476 AAA35564 AAA35565 AAH10264 AAH42354
REF	NP_001006919 NP_001033096 NP_001069862 NP_001071635 NP_001103022
SP	Q07955 Q0VCY7 Q3YLA6 Q5R7H2 Q6DII2
TPG	DAA19172
AlphaFold	Q07955 Q0VCY7 Q3YLA6 Q5R7H2 Q6DII2

1	GLY	ALA	PRO	ARG	GLY	ARG	TYR	GLY	PRO	PRO
2	SER	ARG	ARG	SER	GLU	ASN	ARG	VAL	VAL	VAL
3	SER	GLY	LEU	PRO	PRO	SER	GLY	SER	TRP	GLN
4	ASP	LEU	LYS	ASP	HIS	MET	ARG	GLU	ALA	GLY
5	ASP	VAL	CYS	TYR	ALA	ASP	VAL	TYR	ARG	ASP
6	GLY	THR	GLY	VAL	VAL	GLU	PHE	VAL	ARG	LYS
7	GLU	ASP	MET	THR	TYR	ALA	VAL	ARG	LYS	LEU
8	ASP	ASN	THR	LYS	PHE	ARG	SER	HIS	GLU	GLY
9	GLU	THR	ALA	TYR	ILE	ARG	VAL	LYS	VAL	ASP

1	GLY	ALA	PRO	ARG	GLY	ARG	TYR	GLY	PRO	PRO
2	SER	ARG	ARG	SER	GLU	ASN	ARG	VAL	VAL	VAL
3	SER	GLY	LEU	PRO	PRO	SER	GLY	SER	TRP	GLN
4	ASP	LEU	LYS	ASP	HIS	MET	ARG	GLU	ALA	GLY
5	ASP	VAL	CYS	TYR	ALA	ASP	VAL	TYR	ARG	ASP
6	GLY	THR	GLY	VAL	VAL	GLU	PHE	VAL	ARG	LYS
7	GLU	ASP	MET	THR	TYR	ALA	VAL	ARG	LYS	LEU
8	ASP	ASN	THR	LYS	PHE	ARG	SER	HIS	GLU	GLY
9	GLU	THR	ALA	TYR	ILE	ARG	VAL	LYS	VAL	ASP