BMRB Entry 17526

Name: EcNusB bound to BoxA RNA
Published: 2011-06-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17526

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

EcNusB bound to BoxA RNA

Deposition date:

2011-03-15

Original release date:

2011-06-30

Authors:

Stagno, Jason; Altieri, Amanda; Bubunenko, Mikhail; Tarasov, Sergey; Li, Jess; Court, Donald; Byrd, Andrew; Ji, Xinhua

Citation:

Citation: Stagno, Jason; Altieri, Amanda; Bubunenko, Mikhail; Tarasov, Sergey; Li, Jess; Court, Donald; Byrd, R. Andrew; Ji, Xinhua. "Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination." Nucleic Acids Res. 39, 7803-7815 (2011).
PubMed: 21652641

Assembly members:

Assembly members:
NusB, polymer, 139 residues, Formula weight is not available
BoxA, polymer, . residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NusB: MKPAARRRAREAAVQALYSW QLSQNDIADVEYQFLAEQDV KDVDVLYFRELLAGVATNTA YLDGLMKPYLSRLLEELGQV EKAVLRIALYELSKRSDVPY KVAINEAIELAKSFGAEDSH KFVNGVLDKAAPVIRPNKK
BoxA: UGCUCUUUAACA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	427
15N chemical shifts	135
1H chemical shifts	722

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NusB	1
2	BoxA RNA	2

Entities:

Entity 1, NusB 139 residues - Formula weight is not available

1

MET

LYS

PRO

ALA

ARG

ALA

ARG

2

GLU

ALA

VAL

GLN

ALA

LEU

TYR

SER

TRP

3

GLN

LEU

SER

GLN

ASN

ASP

ILE

ALA

ASP

VAL

4

GLU

TYR

GLN

PHE

LEU

ALA

GLU

GLN

ASP

VAL

5

LYS

ASP

VAL

ASP

VAL

LEU

TYR

PHE

ARG

GLU

6

LEU

ALA

GLY

VAL

ALA

THR

ASN

THR

ALA

7

TYR

LEU

ASP

GLY

LEU

MET

LYS

PRO

TYR

LEU

8

SER

ARG

LEU

GLU

LEU

GLY

GLN

VAL

9

GLU

LYS

ALA

VAL

LEU

ARG

ILE

ALA

LEU

TYR

10

GLU

LEU

SER

LYS

ARG

SER

ASP

VAL

PRO

TYR

11

LYS

VAL

ALA

ILE

ASN

GLU

ALA

ILE

GLU

LEU

12

ALA

LYS

SER

PHE

GLY

ALA

GLU

ASP

SER

HIS

13

LYS

PHE

VAL

ASN

GLY

VAL

LEU

ASP

LYS

ALA

14

ALA

PRO

VAL

ILE

ARG

PRO

ASN

LYS

Entity 2, BoxA RNA - Formula weight is not available

1

U

G

C

U

C

U

A

2

C

A

Samples:

sample_1: E coli NusB, [U-100% 13C; U-100% 15N], 250 uM; boxA RNA, [U-100% 13C; U-100% 15N], 250 uM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz

BMRB	4737
PDB	1EY1 3D3B 3D3C 3IMQ
DBJ	BAB33892 BAE76196 BAG75962 BAI23787 BAI29258
EMBL	CAA25289 CAA45737 CAC44764 CAP74950 CAQ30885
GB	AAA24228 AAB40172 AAB95441 AAC73519 AAG54765
PRF	2111328A
REF	NP_308496 NP_414950 NP_706304 WP_000801121 WP_000801122
SP	A7ZIG9 A7ZX68 B1J033 B1LJG6 B1XF04
AlphaFold	A7ZIG9 A7ZX68 B1J033 B1LJG6 B1XF04