BMRB Entry 17102
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17102
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NMR-STAR v3 text file.
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Citation: Millet, Oscar; Bernado, Pau; Garcia, Jesus; Rizo, Josep; Pons, Miquel. "NMR measurement of the off rate from the first calcium-binding site of the synaptotagmin I C2A domain" FEBS Lett. 516, 93-96 (2002).
PubMed: 11959110
Assembly members:
C2A, polymer, 143 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: purified from the natural source
- chemical_rates
| Data type | Count |
| kinetic rates | 3 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C2A | 1 |
| 2 | CA | 2 |
Entities:
Entity 1, C2A 143 residues - 40.078 Da.
| 1 | GLY | SER | PRO | GLY | ILE | GLY | GLY | GLY | GLY | GLY | ||||
| 2 | GLY | ILE | LEU | ASP | SER | MET | VAL | GLU | LYS | LEU | ||||
| 3 | GLY | LYS | LEU | GLN | TYR | SER | LEU | ASP | TYR | ASP | ||||
| 4 | PHE | GLN | ASN | ASN | GLN | LEU | LEU | VAL | GLY | ILE | ||||
| 5 | ILE | GLN | ALA | ALA | GLU | LEU | PRO | ALA | LEU | ASP | ||||
| 6 | MET | GLY | GLY | THR | SER | ASP | PRO | TYR | VAL | LYS | ||||
| 7 | VAL | PHE | LEU | LEU | PRO | ASP | LYS | LYS | LYS | LYS | ||||
| 8 | PHE | GLU | THR | LYS | VAL | HIS | ARG | LYS | THR | LEU | ||||
| 9 | ASN | PRO | VAL | PHE | ASN | GLU | GLN | PHE | THR | PHE | ||||
| 10 | LYS | VAL | PRO | TYR | SER | GLU | LEU | GLY | GLY | LYS | ||||
| 11 | THR | LEU | VAL | MET | ALA | VAL | TYR | ASP | PHE | ASN | ||||
| 12 | ARG | PHE | SER | LYS | HIS | ASP | ILE | ILE | GLY | GLU | ||||
| 13 | PHE | LYS | VAL | PRO | MET | ASN | THR | VAL | ASP | PHE | ||||
| 14 | GLY | HIS | VAL | THR | GLU | GLU | TRP | ARG | ASP | LEU | ||||
| 15 | GLN | SER | ALA |
Entity 2, CA - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: C2A, [U-15N], 0.93 mM; CA 0.198 mM; EDTA 0.198 mM; Tris 20 mM; sodium chloride 100 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY-HSQC | sample_1 | isotropic | sample_conditions_1 |
| RC-CPMG | sample_1 | isotropic | sample_conditions_1 |
Software:
CurveFit, GraphPad Software, Inc. - relaxation rates and their errors
Microcal_Origin, (Microcal Software) - data analysis
Mathematica, (Wolfram Research) - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 800 MHz