BMRB Entry 17053

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17053

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Title:
A NMR-based docking model for the physiological transient complex between cytochrome f and cytochrome c6
Deposition date:
2010-07-13
Original release date:
2010-09-08
Authors:
Diaz-Moreno, Irene; Diaz-Quintana, Antonio; Ubbink, Marcellus; De la Rosa, Miguel
Citation:

Citation: Diaz-Moreno, Irene; Diaz-Quintana, Antonio; Ubbink, Marcellus; De la Rosa, Miguel. "An NMR-based docking model for the physiological transient complex between cytochrome f and cytochrome c6"  FEBS Lett. 579, 2891-2896 (2005).

Assembly members:

Assembly members:
Cc6, polymer, 80 residues, Formula weight is not available
Cf, polymer, 250 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Nostoc   Taxonomy ID: 1168   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEACWT and pEC86

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cc6: NEQLAKQKGCMACHDLKAKK VGPAYADVAKKYAGRKDAVD YLAGKIKKGGSGVWGSVPMP PQNVTDAEAKQLAQWILSIK
Cf: YPIFAQQNYENPREATGRIV CANCHLASKPVDIEVPQAVL PDTVFEAVVKIPYDMQLKQV LANGKKGALNVGAVLILPEG FELAPPDRISPEMKEKIGNL SFQNYRPNKKNILVIGPVPG QKYSEITFPILAPDPATNKD VHFLKYPIYVGGNRGRGQIY PDGSKSNNTVYNATAGGIIS KILRKEKGGYEITIVDASNE RQVIDIIPRGLELLVSEGES IKLDQPLTSNPNVGGFGQGD AEIVLQDPLR

Data sets:
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cc61
2Cf2

Entities:

Entity 1, Cc6 80 residues - Formula weight is not available

1   ASNGLUGLNLEUALALYSGLNLYSGLYCYS
2   METALACYSHISASPLEULYSALALYSLYS
3   VALGLYPROALATYRALAASPVALALALYS
4   LYSTYRALAGLYARGLYSASPALAVALASP
5   TYRLEUALAGLYLYSILELYSLYSGLYGLY
6   SERGLYVALTRPGLYSERVALPROMETPRO
7   PROGLNASNVALTHRASPALAGLUALALYS
8   GLNLEUALAGLNTRPILELEUSERILELYS

Entity 2, Cf 250 residues - Formula weight is not available

1   TYRPROILEPHEALAGLNGLNASNTYRGLU
2   ASNPROARGGLUALATHRGLYARGILEVAL
3   CYSALAASNCYSHISLEUALASERLYSPRO
4   VALASPILEGLUVALPROGLNALAVALLEU
5   PROASPTHRVALPHEGLUALAVALVALLYS
6   ILEPROTYRASPMETGLNLEULYSGLNVAL
7   LEUALAASNGLYLYSLYSGLYALALEUASN
8   VALGLYALAVALLEUILELEUPROGLUGLY
9   PHEGLULEUALAPROPROASPARGILESER
10   PROGLUMETLYSGLULYSILEGLYASNLEU
11   SERPHEGLNASNTYRARGPROASNLYSLYS
12   ASNILELEUVALILEGLYPROVALPROGLY
13   GLNLYSTYRSERGLUILETHRPHEPROILE
14   LEUALAPROASPPROALATHRASNLYSASP
15   VALHISPHELEULYSTYRPROILETYRVAL
16   GLYGLYASNARGGLYARGGLYGLNILETYR
17   PROASPGLYSERLYSSERASNASNTHRVAL
18   TYRASNALATHRALAGLYGLYILEILESER
19   LYSILELEUARGLYSGLULYSGLYGLYTYR
20   GLUILETHRILEVALASPALASERASNGLU
21   ARGGLNVALILEASPILEILEPROARGGLY
22   LEUGLULEULEUVALSERGLUGLYGLUSER
23   ILELYSLEUASPGLNPROLEUTHRSERASN
24   PROASNVALGLYGLYPHEGLYGLNGLYASP
25   ALAGLUILEVALLEUGLNASPPROLEUARG

Samples:

sample_1: sodium phosphate 10 mM; Cc6, [U-15N], 0.2 mM; Cf 3.7 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

AZARA, Boucher - processing

ANSIG, Kraulis - data analysis

ORIGIN v6.0, (Microcal Inc., USA) - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 10134 10135 25389 25390
PDB
DBJ BAI69446 BAA84398 BAF49783 BAF49867 BAF49952 BAF50036
EMBL CAA40902 CAA54307 CCW28192 CDX92360 CDX99939 CDY20523
GB ADO45379 ACY66226 AFM92292 AFQ07804 AIE42479 AIK29017
REF WP_012963626 NP_051072 XP_002888345 YP_001122959 YP_001123043 YP_001123128
SP P15452 A4QJC8 A4QJL2 A4QJZ7 A4QK31 A4QKB8
AlphaFold P15452 A4QJC8 A4QJL2 A4QJZ7 A4QK31 A4QKB8