BMRB Entry 16753
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16753
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NMR-STAR v3 text file.
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Citation: Tosha, Takehiko; Yoshioka, Shiro; Takahashi, Satoshi; Ishimori, Koichiro; Shimada, Hideo; Morishima, Isao. "NMR Study on the Structural Changes of Cytochrome P450cam on the Complex Formation with Putidaredoxin" J. Biol. Chem. 278, 39809-39821 (2003).
PubMed: 12842870
Assembly members:
P450cam, polymer, 414 residues, Formula weight is not available
Pdx, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas putida
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na
Entity Sequences (FASTA):
P450cam: TTETIQSNANLAPLPPHVPE
HLVFDFDMYNPSNLSAGVQE
AWAVLQESNVPDLVWTRCNG
GHWIATRGQLIREAYEDYRH
FSSECPFIPREAGEAYDFIP
TSMDPPEQRQFRALANQVVG
MPVVDKLENRIQELACSLIE
SLRPQGQCNFTEDYAEPFPI
RIFMLLAGLPEEDIPHLKYL
TDQMTRPDGSMTFAEAKEAL
YDYLIPIIEQRRQKPGTDAI
SIVANGQVNGRPITSDEAKR
MCGLLLVGGLDTVVNFLSFS
MEFLAKSPEHRQELIQRPER
IPAACEELLRRFSLVADGRI
LTSDYEFHGVQLKKGDQILL
PQMLSGLDERENACPMHVDF
SRQKVSHTTFGHGSHLCLGQ
HLARREIIVTLKEWLTRIPD
FSIAPGAQIQHKSGIVSGVQ
ALPLVWDPATTKAV
Pdx: SKVVYVSHDGTRRQLDVADG
VSLMQAAVSNGIYDIVGDCG
GSASCATCHVYVNEAFTDKV
PAANEREIGMLECVTAELKP
NSRLCCQIIMTPELDGIVVD
VPDRQW
- binding_constants
| Data type | Count |
| binding constants | 4 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | P450cam | 1 |
| 2 | Pdx | 2 |
Entities:
Entity 1, P450cam 414 residues - Formula weight is not available
| 1 | THR | THR | GLU | THR | ILE | GLN | SER | ASN | ALA | ASN | ||||
| 2 | LEU | ALA | PRO | LEU | PRO | PRO | HIS | VAL | PRO | GLU | ||||
| 3 | HIS | LEU | VAL | PHE | ASP | PHE | ASP | MET | TYR | ASN | ||||
| 4 | PRO | SER | ASN | LEU | SER | ALA | GLY | VAL | GLN | GLU | ||||
| 5 | ALA | TRP | ALA | VAL | LEU | GLN | GLU | SER | ASN | VAL | ||||
| 6 | PRO | ASP | LEU | VAL | TRP | THR | ARG | CYS | ASN | GLY | ||||
| 7 | GLY | HIS | TRP | ILE | ALA | THR | ARG | GLY | GLN | LEU | ||||
| 8 | ILE | ARG | GLU | ALA | TYR | GLU | ASP | TYR | ARG | HIS | ||||
| 9 | PHE | SER | SER | GLU | CYS | PRO | PHE | ILE | PRO | ARG | ||||
| 10 | GLU | ALA | GLY | GLU | ALA | TYR | ASP | PHE | ILE | PRO | ||||
| 11 | THR | SER | MET | ASP | PRO | PRO | GLU | GLN | ARG | GLN | ||||
| 12 | PHE | ARG | ALA | LEU | ALA | ASN | GLN | VAL | VAL | GLY | ||||
| 13 | MET | PRO | VAL | VAL | ASP | LYS | LEU | GLU | ASN | ARG | ||||
| 14 | ILE | GLN | GLU | LEU | ALA | CYS | SER | LEU | ILE | GLU | ||||
| 15 | SER | LEU | ARG | PRO | GLN | GLY | GLN | CYS | ASN | PHE | ||||
| 16 | THR | GLU | ASP | TYR | ALA | GLU | PRO | PHE | PRO | ILE | ||||
| 17 | ARG | ILE | PHE | MET | LEU | LEU | ALA | GLY | LEU | PRO | ||||
| 18 | GLU | GLU | ASP | ILE | PRO | HIS | LEU | LYS | TYR | LEU | ||||
| 19 | THR | ASP | GLN | MET | THR | ARG | PRO | ASP | GLY | SER | ||||
| 20 | MET | THR | PHE | ALA | GLU | ALA | LYS | GLU | ALA | LEU | ||||
| 21 | TYR | ASP | TYR | LEU | ILE | PRO | ILE | ILE | GLU | GLN | ||||
| 22 | ARG | ARG | GLN | LYS | PRO | GLY | THR | ASP | ALA | ILE | ||||
| 23 | SER | ILE | VAL | ALA | ASN | GLY | GLN | VAL | ASN | GLY | ||||
| 24 | ARG | PRO | ILE | THR | SER | ASP | GLU | ALA | LYS | ARG | ||||
| 25 | MET | CYS | GLY | LEU | LEU | LEU | VAL | GLY | GLY | LEU | ||||
| 26 | ASP | THR | VAL | VAL | ASN | PHE | LEU | SER | PHE | SER | ||||
| 27 | MET | GLU | PHE | LEU | ALA | LYS | SER | PRO | GLU | HIS | ||||
| 28 | ARG | GLN | GLU | LEU | ILE | GLN | ARG | PRO | GLU | ARG | ||||
| 29 | ILE | PRO | ALA | ALA | CYS | GLU | GLU | LEU | LEU | ARG | ||||
| 30 | ARG | PHE | SER | LEU | VAL | ALA | ASP | GLY | ARG | ILE | ||||
| 31 | LEU | THR | SER | ASP | TYR | GLU | PHE | HIS | GLY | VAL | ||||
| 32 | GLN | LEU | LYS | LYS | GLY | ASP | GLN | ILE | LEU | LEU | ||||
| 33 | PRO | GLN | MET | LEU | SER | GLY | LEU | ASP | GLU | ARG | ||||
| 34 | GLU | ASN | ALA | CYS | PRO | MET | HIS | VAL | ASP | PHE | ||||
| 35 | SER | ARG | GLN | LYS | VAL | SER | HIS | THR | THR | PHE | ||||
| 36 | GLY | HIS | GLY | SER | HIS | LEU | CYS | LEU | GLY | GLN | ||||
| 37 | HIS | LEU | ALA | ARG | ARG | GLU | ILE | ILE | VAL | THR | ||||
| 38 | LEU | LYS | GLU | TRP | LEU | THR | ARG | ILE | PRO | ASP | ||||
| 39 | PHE | SER | ILE | ALA | PRO | GLY | ALA | GLN | ILE | GLN | ||||
| 40 | HIS | LYS | SER | GLY | ILE | VAL | SER | GLY | VAL | GLN | ||||
| 41 | ALA | LEU | PRO | LEU | VAL | TRP | ASP | PRO | ALA | THR | ||||
| 42 | THR | LYS | ALA | VAL |
Entity 2, Pdx 106 residues - Formula weight is not available
106 residues of oxidized form given
| 1 | SER | LYS | VAL | VAL | TYR | VAL | SER | HIS | ASP | GLY | ||||
| 2 | THR | ARG | ARG | GLN | LEU | ASP | VAL | ALA | ASP | GLY | ||||
| 3 | VAL | SER | LEU | MET | GLN | ALA | ALA | VAL | SER | ASN | ||||
| 4 | GLY | ILE | TYR | ASP | ILE | VAL | GLY | ASP | CYS | GLY | ||||
| 5 | GLY | SER | ALA | SER | CYS | ALA | THR | CYS | HIS | VAL | ||||
| 6 | TYR | VAL | ASN | GLU | ALA | PHE | THR | ASP | LYS | VAL | ||||
| 7 | PRO | ALA | ALA | ASN | GLU | ARG | GLU | ILE | GLY | MET | ||||
| 8 | LEU | GLU | CYS | VAL | THR | ALA | GLU | LEU | LYS | PRO | ||||
| 9 | ASN | SER | ARG | LEU | CYS | CYS | GLN | ILE | ILE | MET | ||||
| 10 | THR | PRO | GLU | LEU | ASP | GLY | ILE | VAL | VAL | ASP | ||||
| 11 | VAL | PRO | ASP | ARG | GLN | TRP |
Samples:
sample_1: P450cam 0.2 mM; potassium phosphate 50 mM; potassium chloride 50 mM; d-camphor 1 mM; Pdx0.1 – 0.8 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H NMR | sample_1 | isotropic | sample_conditions_1 |
Software:
InsightII, Accelrys Software Inc. - model drawing
NMR spectrometers:
- Bruker Avance DRX 500 MHz
- Bruker Avance DRX 600 MHz