BMRB Entry 16417

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16417

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Title:
An Atomic Resolution Model for Assembly, Architecture, and Function of the Dr Adhesins
Deposition date:
2009-07-23
Original release date:
2010-03-08
Authors:
Anderson, Kirstine; Billington, Jason; Pettigrew, David; Cota, Ernesto; Simpson, Peter; Roversi, Pietro; Chen, Ho An; Urvil, Petri; du Merle, Laurence; Barlow, Peter; Medof, M.; Smith, Richard; Nowicki, Bogdan; Le Bouguenec, Chantal; Lea, Susan; Matthews, Stephen
Citation:

Citation: Anderson, Kirstine; Billington, Jason; Pettigrew, David; Cota, Ernesto; Urvil, Petri; Simpson, Peter; Roversi, Pietro; Chen, Ho An; du Merle, Laurence; Barlow, Paul; Medof, M.; Smith, Richard; Nowicki, Bogdan; Le Bouguenec, Chantal; Lea, Susan; Matthews, Stephen. "An Atomic Resolution Model for Assembly, Architecture, and Function of the Dr Adhsins"  Mol. Cell 15, 647-657 (2004).
PubMed: 15327779

Assembly members:

Assembly members:
AfaE-dsc, polymer, 143 residues, Formula weight is not available
DAF1234, polymer, 254 residues, 70000 Da.
DAF, polymer, 254 residues, 57794.34 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AfaE-dsc: EECQVRVGDLTVAKTRGQLT DAAPIGPVTVQALGCNARQV ALKADTDNFEQGKFFLISDN NRDKLYVNIRPMDNSAWTTD NGVFYKNDVGSWGGTIGIYV DGQQTNTPPGNYTLTLTGGY WAKDNKQGFTPSGTTGTTKL TVT
DAF1234: MQDCGLPPDVPNAQPALEGR TSFPEDTVITYKCEESFVKI PGEKDSVICLKGSQWSDIEE FCNRSCEVPTRLNSASLKQP YITQNYFPVGTVVEYECRPG YRREPSLSPKLTCLQNLKWS TAVEFCKKKSCPNPGEIRNG QIDVPGGILFGATISFSCNT GYKLFGSTSSFCLISGSSVQ WSDPLPECREIYCPAPPQID NGIIQGERDHYGYRQSVTYA CNKGFTMIGEHSIYCTVNND EGEWSGPPPECRGC
DAF: MQDCGLPPDVPNAQPALEGR TSFPEDTVITYKCEESFVKI PGEKDSVICLKGSQWSDIEE FCNRSCEVPTRLNSASLKQP YITQNYFPVGTVVEYECRPG YRREPSLSPKLTCLQNLKWS TAVEFCKKKSCPNPGEIRNG QIDVPGGILFGATISFSCNT GYKLFGSTSSFCLISGSSVQ WSDPLPECREIYCPAPPQID NGIIQGERDHYGYRQSVTYA CNKGFTMIGEHSIYCTVNND EGEWSGPPPECRGC

Data sets:
  • binding_constants
Data typeCount
binding constants4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AfaE-dsc1
2DAF12342

Entities:

Entity 1, AfaE-dsc 143 residues - Formula weight is not available

1   GLUGLUCYSGLNVALARGVALGLYASPLEU
2   THRVALALALYSTHRARGGLYGLNLEUTHR
3   ASPALAALAPROILEGLYPROVALTHRVAL
4   GLNALALEUGLYCYSASNALAARGGLNVAL
5   ALALEULYSALAASPTHRASPASNPHEGLU
6   GLNGLYLYSPHEPHELEUILESERASPASN
7   ASNARGASPLYSLEUTYRVALASNILEARG
8   PROMETASPASNSERALATRPTHRTHRASP
9   ASNGLYVALPHETYRLYSASNASPVALGLY
10   SERTRPGLYGLYTHRILEGLYILETYRVAL
11   ASPGLYGLNGLNTHRASNTHRPROPROGLY
12   ASNTYRTHRLEUTHRLEUTHRGLYGLYTYR
13   TRPALALYSASPASNLYSGLNGLYPHETHR
14   PROSERGLYTHRTHRGLYTHRTHRLYSLEU
15   THRVALTHR

Entity 2, DAF1234 254 residues - 70000 Da.

1   METGLNASPCYSGLYLEUPROPROASPVAL
2   PROASNALAGLNPROALALEUGLUGLYARG
3   THRSERPHEPROGLUASPTHRVALILETHR
4   TYRLYSCYSGLUGLUSERPHEVALLYSILE
5   PROGLYGLULYSASPSERVALILECYSLEU
6   LYSGLYSERGLNTRPSERASPILEGLUGLU
7   PHECYSASNARGSERCYSGLUVALPROTHR
8   ARGLEUASNSERALASERLEULYSGLNPRO
9   TYRILETHRGLNASNTYRPHEPROVALGLY
10   THRVALVALGLUTYRGLUCYSARGPROGLY
11   TYRARGARGGLUPROSERLEUSERPROLYS
12   LEUTHRCYSLEUGLNASNLEULYSTRPSER
13   THRALAVALGLUPHECYSLYSLYSLYSSER
14   CYSPROASNPROGLYGLUILEARGASNGLY
15   GLNILEASPVALPROGLYGLYILELEUPHE
16   GLYALATHRILESERPHESERCYSASNTHR
17   GLYTYRLYSLEUPHEGLYSERTHRSERSER
18   PHECYSLEUILESERGLYSERSERVALGLN
19   TRPSERASPPROLEUPROGLUCYSARGGLU
20   ILETYRCYSPROALAPROPROGLNILEASP
21   ASNGLYILEILEGLNGLYGLUARGASPHIS
22   TYRGLYTYRARGGLNSERVALTHRTYRALA
23   CYSASNLYSGLYPHETHRMETILEGLYGLU
24   HISSERILETYRCYSTHRVALASNASNASP
25   GLUGLYGLUTRPSERGLYPROPROPROGLU
26   CYSARGGLYCYS

Samples:

sample_1: AfaE-dsc, [U-13C; U-15N], 30 uM; sodium acetate 50 mM; DAF, [U-15N], 0 – 30 uM

sample_2: AfaE-dsc 30 uM; sodium acetate 50 mM; DAF, [U-15N], 0 – 30 uM

sample_3: AfaE-dsc, [U-13C; U-15N], 30 uM; sodium acetate 50 mM; DAF1234, [U-15N], 0 – 30 uM

sample_4: AfaE-dsc 30 uM; sodium acetate 50 mM; DAF1234, [U-15N], 0 – 30 uM

sample_conditions_1: pH: 5.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HNCOsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - data analysis, structure solution

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAA48847 CAA54121
GB AAA23709 AAK16480 ABD93399 ABD93405 ABD93406 AAA52167 AAA52168 AAA52169 AAB48622 AAF73175 AAA52167 AAA52168 AAA52169 AAB48622 AAF73175
REF WP_021517429 WP_021523428 WP_021566292 NP_000565 NP_001108224 NP_001287831 NP_001287832 NP_001287833 NP_000565 NP_001108224 NP_001287831 NP_001287832 NP_001287833
SP P24093 Q57254 P08174 P08174
DBJ BAE97422 BAE97423 BAE97424 BAE97425 BAE97426 BAE97422 BAE97423 BAE97424 BAE97425 BAE97426
PRF 1303335A 1303335B 1303335A 1303335B
AlphaFold P24093 Q57254 P08174 P08174