BMRB Entry 15279

Name: Structure of the EH-domain of EHD1
Published: 2007-10-16

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PDB ID: 2jq6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15279
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the EH-domain of EHD1

Deposition date:

2007-06-01

Original release date:

2007-10-16

Authors:

KIEKEN, Fabien; JOVIC, Marko; CAPLAN, Steve; SORGEN, Paul

Citation:

Citation: Kieken, Fabien; Jovic, Marko; Naslavsky, N.; Caplan, Steve; Sorgen, Paul. "EH domain of EHD1" J. Biomol. NMR 39, 323-329 (2007).
PubMed: 17899392

Assembly members:

Assembly members:
EH domain of EHD1, polymer, 139 residues, 15004.205 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pgex 6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EH domain of EHD1: GPLGSESLMPSQVVKGGAFD GTMNGPFGHGYGEGAGEGID DVEWVVGKDKPTYDEIFYTL SPVNGKITGANAKKEMVKSK LPNTVLGKIWKLADVDKDGL LDDEEFALANHLIKVKLEGH ELPADLPPHLVPPSKRRHE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	602
15N chemical shifts	131
1H chemical shifts	967

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EH domain of EHD1	1
2	CALCIUM ION	2

Entities:

Entity 1, EH domain of EHD1 139 residues - 15004.205 Da.

1

GLY

PRO

LEU

GLY

SER

GLU

SER

LEU

MET

PRO

2

SER

GLN

VAL

LYS

GLY

ALA

PHE

ASP

3

GLY

THR

MET

ASN

GLY

PRO

PHE

GLY

HIS

GLY

4

TYR

GLY

GLU

GLY

ALA

GLY

GLU

GLY

ILE

ASP

5

ASP

VAL

GLU

TRP

VAL

GLY

LYS

ASP

LYS

6

PRO

THR

TYR

ASP

GLU

ILE

PHE

TYR

THR

LEU

7

SER

PRO

VAL

ASN

GLY

LYS

ILE

THR

GLY

ALA

8

ASN

ALA

LYS

GLU

MET

VAL

LYS

SER

LYS

9

LEU

PRO

ASN

THR

VAL

LEU

GLY

LYS

ILE

TRP

10

LYS

LEU

ALA

ASP

VAL

ASP

LYS

ASP

GLY

LEU

11

LEU

ASP

GLU

PHE

ALA

LEU

ALA

ASN

12

HIS

LEU

ILE

LYS

VAL

LYS

LEU

GLU

GLY

HIS

13

GLU

LEU

PRO

ALA

ASP

LEU

PRO

HIS

LEU

14

VAL

PRO

SER

LYS

ARG

HIS

GLU

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: EH Domain of EHD-1, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; CALCIUM ION 3 mM; PBS 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

ARIA v1.2, Linge, O'Donoghue and Nilges - refinement

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16179 16180 16181 16671
PDB	2JQ6 2KFF 2KFG 2KFH 2KSP
DBJ	BAB28540 BAC40684 BAE32742 BAE35499 BAE35852
EMBL	CAH90816
GB	AAB81204 AAD45423 AAD45866 AAF24223 AAG02009
REF	NP_001011939 NP_001015578 NP_001125465 NP_001162473 NP_001248124
SP	Q5E9R3 Q5RBP4 Q641Z6 Q9H4M9 Q9WVK4
AlphaFold	Q5E9R3 Q5RBP4 Q641Z6 Q9H4M9 Q9WVK4