BMRB Entry 11400

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11400

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the RNA recognition motif in Arginine/serine-rich splicing factor 10

Deposition date:

2010-09-09

Original release date:

2011-09-08

Authors:

Someya, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Someya, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution Structure of the RNA recognition motif in Arginine/serine-rich splicing factor 10" .

Assembly members:

Assembly members:
RNA recognition motif, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040517-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA recognition motif: GSSGSSGNRANPDPNCCLGV FGLSLYTTERDLREVFSKYG PIADVSIVYDQQSRRSRGFA FVYFENVDDAKEAKERANGM ELDGRRIRVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	365
15N chemical shifts	84
1H chemical shifts	564

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA recognition motif	1

Entities:

Entity 1, RNA recognition motif 95 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

ARG

ALA

2

ASN

PRO

ASP

PRO

ASN

CYS

LEU

GLY

VAL

3

PHE

GLY

LEU

SER

LEU

TYR

THR

GLU

ARG

4

ASP

LEU

ARG

GLU

VAL

PHE

SER

LYS

TYR

GLY

5

PRO

ILE

ALA

ASP

VAL

SER

ILE

VAL

TYR

ASP

6

GLN

SER

ARG

SER

ARG

GLY

PHE

ALA

7

PHE

VAL

TYR

PHE

GLU

ASN

VAL

ASP

ALA

8

LYS

GLU

ALA

LYS

GLU

ARG

ALA

ASN

GLY

MET

9

GLU

LEU

ASP

GLY

ARG

ILE

ARG

VAL

SER

10

GLY

PRO

SER

GLY

Samples:

sample_1: RNA recognition motif, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

BMRB	11409 11410 11411 11412 16920
PDB	2CQC 2KXN 2RRA
DBJ	BAA08556 BAC05256 BAC33819 BAD92445 BAE88784
EMBL	CAA56518 CAH18071 CAH90742
GB	AAB08701 AAC28242 AAD19277 AAD19278 AAG35783
REF	NP_001006878 NP_001029948 NP_001070689 NP_001125414 NP_001230808
SP	P62995 P62996 P62997 Q3ZBT6
TPG	DAA33375
AlphaFold	P62995 P62996 P62997 Q3ZBT6