BMRB Entry 7120

Name: The SAM domain of DLC1 defines a novel structure that interacts with elongation factor 1A1 at cortical actin and membrane ruffles
Published: 2009-05-27

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PDB ID: 2gyt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR7120
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The SAM domain of DLC1 defines a novel structure that interacts with elongation factor 1A1 at cortical actin and membrane ruffles

Deposition date:

2006-05-18

Original release date:

2009-05-27

Authors:

Yang, S.

Citation:

Citation: Zhong, D.; Yang, S.; Zhang, J.; Buschdorf, J.; Yang, D.; Low, B.. "The SAM domain of DLC1 defines a novel structure that interacts with elongation factor 1A1 at cortical actin and membrane ruffles" .

Assembly members:

Assembly members:
Rho-GTPase-activating protein 7, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rho-GTPase-activating protein 7: MCRKKPDTMILTQIEAKEAC DWLRATGFPQYAQLYEDFLF PIDISLVKREHDFLDRDAIE ALCRRLNTLNKCAVMK

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	256
15N chemical shifts	76
1H chemical shifts	511

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Deleted in liver cancer 1 protein, isoform 2	1

Entities:

Entity 1, Deleted in liver cancer 1 protein, isoform 2 76 residues - Formula weight is not available

1

MET

CYS

ARG

LYS

PRO

ASP

THR

MET

ILE

2

LEU

THR

GLN

ILE

GLU

ALA

LYS

GLU

ALA

CYS

3

ASP

TRP

LEU

ARG

ALA

THR

GLY

PHE

PRO

GLN

4

TYR

ALA

GLN

LEU

TYR

GLU

ASP

PHE

LEU

PHE

5

PRO

ILE

ASP

ILE

SER

LEU

VAL

LYS

ARG

GLU

6

HIS

ASP

PHE

LEU

ASP

ARG

ASP

ALA

ILE

GLU

7

ALA

LEU

CYS

ARG

LEU

ASN

THR

LEU

ASN

8

LYS

CYS

ALA

VAL

MET

LYS

Samples:

sample_1: Rho-GTPase-activating protein 7, [U-15N; U-13C], 1 mM; sodium phosphate buffer 50 mM; DTT 3 mM; H2O 90%; D2O 10%

sample_2: Rho-GTPase-activating protein 7, [U-15N], 1 mM; sodium phosphate buffer 50 mM; DTT 3 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	sample_cond_1
3D 15N-separated NOESY	not available	not available	sample_cond_1

Software:

xwinnmr v3.5 - collection

NMRView v5.2.2_01 - data analysis

NMRPipe v2004.126.16.02 - processing

AMBER v7 - refinement

TALOS v2003.027.13.05 - refinement

CYANA v1.0.5 - structure solution

NMR spectrometers:

Bruker AVANCE 500 MHz

PDB	2DKY 2GYT 2KAP
DBJ	BAB21814 BAF82423 BAF84371 BAG10131
GB	AAB81637 AAB87700 AAH54511 AAK97501 ACE86794
PIR	G59435
REF	NP_006085 NP_872584 XP_001092830 XP_002919590 XP_003256738
SP	Q96QB1
AlphaFold	Q96QB1