BMRB Entry 6904

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6904

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C and 15N resonance assignment of M-crystallin: a novel Ca2+ binding protein of the bg-crystallin superfamily from Methanosarcina acetivorans
Deposition date:
2005-11-17
Original release date:
2008-09-02
Authors:
Barnwal, Ravi; Jobby, M.; Sharma, Yogendra; Chary, Kandala
Citation:

Citation: Barnwal, Ravi; Jobby, M.; Sharma, Yogendra; Chary, Kandala. "NMR Assignment of M-crystallin: A Novel Ca(2+) Binding Protein of the betagamma-crystallin Superfamily from Methanosarcina acetivorans."  J. Biomol. NMR 36, 32-32 (2006).
PubMed: 16607466

Assembly members:

Assembly members:
M-Crystallin, polymer, 85 residues, 9376.2 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Methanosarcina acetivorans   Taxonomy ID: 2214   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanosarcina acetivorans

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M-Crystallin: MNAAEVIVYEHVNFGGKSFD ATSDQPGAGDNLNDKISSIK VKSGTWRFYEYINYGGRYWD LGPGEYSSVESAGIPDNSIS SFRQI

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts80
1H chemical shifts509

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1M-Crystallin1
2CALCIUM (II) ION2

Entities:

Entity 1, M-Crystallin 85 residues - 9376.2 Da.

1   METASNALAALAGLUVALILEVALTYRGLU
2   HISVALASNPHEGLYGLYLYSSERPHEASP
3   ALATHRSERASPGLNPROGLYALAGLYASP
4   ASNLEUASNASPLYSILESERSERILELYS
5   VALLYSSERGLYTHRTRPARGPHETYRGLU
6   TYRILEASNTYRGLYGLYARGTYRTRPASP
7   LEUGLYPROGLYGLUTYRSERSERVALGLU
8   SERALAGLYILEPROASPASNSERILESER
9   SERPHEARGGLNILE

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

Sample_1: M-Crystallin, [U-99% 15N], 1.1 mM; CALCIUM (II) ION 6.6 mM

Sample_2: M-Crystallin, [U-99% 13C; U-99% 15N], 0.9 mN; CALCIUM (II) ION 5.4 mM

Cond_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availableCond_1
HN(CA)COnot availablenot availableCond_1
CBCANHnot availablenot availableCond_1
CBCA(CO)NHnot availablenot availableCond_1
1H-15N TOCSY-HSQCnot availablenot availableCond_1
1H-15N NOESY-HSQCnot availablenot availableCond_1

Software:

FELIX v97 software -

TATAPRO v97 software -

NMR spectrometers:

  • Bruker Biospin 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15918 15934 6903
PDB
GB AAM05909
REF NP_617429 WP_011022494

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks