BMRB Entry 6418

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6418

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Title:
Backbone 1H, 13C and 15N resonance assignments for the Mg2+-bound form of the Ca2+-binding photoprotein aequorin
Deposition date:
2004-12-09
Original release date:
2005-07-26
Authors:
Ohashi, Wakana; Inouye, Satoshi; Yamazaki, Toshio; Doi-katayama, Yukiko; Yokoyama, Shigeyuki; Hirota, Hiroshi
Citation:

Citation: Ohashi, Wakana; Inouye, Satoshi; Yamazaki, Toshio; Doi-Katayama, Yukiko; Yokoyama, Shigeyuki; Hirota, Hiroshi. "Letter to the Editor: Backbone 1H, 13C and 15N resonance assignments for the Mg2+ -bound form of the Ca2+ -binding photoprotein aequorin"  J. Biomol. NMR 31, 375-376 (2005).

Assembly members:

Assembly members:
aequorin, polymer, 191 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.
CZH, non-polymer, 455.462 Da.

Natural source:

Natural source:   Common Name: jellyfish   Taxonomy ID: 168712   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: aequorea aequorea

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
aequorin: ANSKLTSDFDNPRWIGRHKH MFNFLDVNHNGKISLDEMVY KASDIVINNLGATPEQAKRH KDAVEAFFGGAGMKYGVETD WPAYIEGWKKLATDELEKYA KNEPTLIRIWGDALFDIVDK DQNGAITLDEWKAYTKAAGI IQSSEDCEETFRVCDIDESG QLDVDEMTRQHLGFWYTMDP ACEKLYGGAVP

Data sets:
Data typeCount
13C chemical shifts540
15N chemical shifts179
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aequorin1
2Mg2+2
3CZH3

Entities:

Entity 1, aequorin 191 residues - Formula weight is not available

1   ALAASNSERLYSLEUTHRSERASPPHEASP
2   ASNPROARGTRPILEGLYARGHISLYSHIS
3   METPHEASNPHELEUASPVALASNHISASN
4   GLYLYSILESERLEUASPGLUMETVALTYR
5   LYSALASERASPILEVALILEASNASNLEU
6   GLYALATHRPROGLUGLNALALYSARGHIS
7   LYSASPALAVALGLUALAPHEPHEGLYGLY
8   ALAGLYMETLYSTYRGLYVALGLUTHRASP
9   TRPPROALATYRILEGLUGLYTRPLYSLYS
10   LEUALATHRASPGLULEUGLULYSTYRALA
11   LYSASNGLUPROTHRLEUILEARGILETRP
12   GLYASPALALEUPHEASPILEVALASPLYS
13   ASPGLNASNGLYALAILETHRLEUASPGLU
14   TRPLYSALATYRTHRLYSALAALAGLYILE
15   ILEGLNSERSERGLUASPCYSGLUGLUTHR
16   PHEARGVALCYSASPILEASPGLUSERGLY
17   GLNLEUASPVALASPGLUMETTHRARGGLN
18   HISLEUGLYPHETRPTYRTHRMETASPPRO
19   ALACYSGLULYSLEUTYRGLYGLYALAVAL
20   PRO

Entity 2, Mg2+ - Mg - 24.305 Da.

1   MG

Entity 3, CZH - C26 H21 N3 O5 - 455.462 Da.

1   CZH

Samples:

sample_1: aequorin, [U-13C; U-15N], 0.8 mM; MES, [U-2H], 10 mM; EDTA 3 mM; KCl 100 mM; MgCl2 10 mM; DTT, [U-2H], 10 mM; D2O 10%; H2O 90%

sample_2: aequorin, [15N]-Met, 0.2 mM; MES, [U-2H], 10 mM; EDTA 3 mM; KCl 100 mM; DTT, [U-2H], 10 mM; MgCl2 10 mM; D2O 10%; H2O 90%

1: ionic strength: 240 mM; pH: 6.6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY-HSQCnot availablenot available1
HNCOnot availablenot available1
HNCACOnot availablenot available1
HNCAnot availablenot available1
HNCOCAnot availablenot available1
CBCACONHnot availablenot available1
HNCACBnot availablenot available1
CCONHnot availablenot available1
13C, 1H-HSQCnot availablenot available1
15N, 1H-HSQCnot availablenot available1

Software:

NMRPipe - data processing

NMRView - data analysis

Kujira - data analysis

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC81729 BAC81731 BAC81732
GB AAA27717 AAA27718 AAA27719 AAA27720 AAT11852
SP P02592
AlphaFold P02592

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks