BMRB Entry 6391
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6391
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Markus, Michelle; Dwyer, Brian; Wolfrom, Scott; Li, Jianchang; Li, Wei; Malakian, Karl; Wilhelm, James; Tsao, Desiree. "1H, 13C, and 15N assignments of MMP-12, a key protease
implicated in lung tissue remodeling" J. Biomol. NMR 31, 260-260 (2005).
Assembly members:
mmp12, polymer, 165 residues, 18342.6 Da.
DSV, non-polymer, 363.451 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 663 |
| 15N chemical shifts | 169 |
| 1H chemical shifts | 980 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mmp12 | 1 |
| 2 | inhibitor | 2 |
Entities:
Entity 1, mmp12 165 residues - 18342.6 Da.
| 1 | MET | PHE | ARG | GLU | MET | PRO | GLY | GLY | PRO | VAL | ||||
| 2 | TRP | ARG | LYS | HIS | TYR | ILE | THR | TYR | ARG | ILE | ||||
| 3 | ASN | ASN | TYR | THR | PRO | ASP | MET | ASN | ARG | GLU | ||||
| 4 | ASP | VAL | ASP | TYR | ALA | ILE | ARG | LYS | ALA | PHE | ||||
| 5 | GLN | VAL | TRP | SER | ASN | VAL | THR | PRO | LEU | LYS | ||||
| 6 | PHE | SER | LYS | ILE | ASN | THR | GLY | MET | ALA | ASP | ||||
| 7 | ILE | LEU | VAL | VAL | PHE | ALA | ARG | GLY | ALA | HIS | ||||
| 8 | GLY | ASP | PHE | HIS | ALA | PHE | ASP | GLY | LYS | GLY | ||||
| 9 | GLY | ILE | LEU | ALA | HIS | ALA | PHE | GLY | PRO | GLY | ||||
| 10 | SER | GLY | ILE | GLY | GLY | ASP | ALA | HIS | PHE | ASP | ||||
| 11 | GLU | ASP | GLU | PHE | TRP | THR | THR | HIS | SER | GLY | ||||
| 12 | GLY | THR | ASN | LEU | PHE | LEU | THR | ALA | VAL | HIS | ||||
| 13 | GLU | ILE | GLY | HIS | SER | LEU | GLY | LEU | GLY | HIS | ||||
| 14 | SER | SER | ASP | PRO | LYS | ALA | VAL | MET | PHE | PRO | ||||
| 15 | THR | TYR | LYS | TYR | VAL | ASP | ILE | ASN | THR | PHE | ||||
| 16 | ARG | LEU | SER | ALA | ASP | ASP | ILE | ARG | GLY | ILE | ||||
| 17 | GLN | SER | LEU | TYR | GLY |
Entity 2, inhibitor - C17 H17 N O4 S2 - 363.451 Da.
| 1 | DSV |
Samples:
15N_sample: mmp12, [U-15N], 0.2 mM; inhibitor 0.2 mM
13C15N_sample: mmp12, [U-13C; U-15N], 0.7 mM; inhibitor 0.7 mM
13C15N_D2O_sample: mmp12, [U-13C; U-15N], 0.4 mM; inhibitor 0.4 mM; D2O 100%
10percent_13C_sample: mmp12, [U-10% 13C], 0.55 mM; inhibitor 0.55 mM
conditions: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | conditions |
| HNCA | not available | not available | conditions |
| HNCACB | not available | not available | conditions |
| CBCA(CO)NH | not available | not available | conditions |
| HN(CA)CO | not available | not available | conditions |
| C(CO)NH | not available | not available | conditions |
| H(CCO)NH | not available | not available | conditions |
| HBHA(CO)NH | not available | not available | conditions |
| HCCH TOCSY | not available | not available | conditions |
| HACAHB | not available | not available | conditions |
| HNHB | not available | not available | conditions |
| 1H13C HSQC | not available | not available | conditions |
Software:
PIPP v4.2.2, Dan Garrett - peak picking
NMRPipe v2.3, Frank Delaglio - processing spectra
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 15578 6444 7089 7415 |
| PDB | |
| DBJ | BAG36675 BAJ20684 |
| GB | AAA58658 AAI12302 AAI43774 AAW29944 ADR83017 |
| REF | NP_002417 XP_003828422 XP_004052087 XP_508724 |
| SP | P39900 |
| AlphaFold | P39900 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks