BMRB Entry 6201

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PDB ID: 1t6w
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6201
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence specific 1H and 15N resonance assignment of domain 1 of rat CD2 with the designed calcium binding site

Deposition date:

2004-05-07

Original release date:

2005-02-16

Authors:

Yang, Wei; Yang, Jenny

Citation:

Citation: Yang, Wei; Wilkins, A.; Ye, Y.; Liu, Z.; Li, S.; Urbauer, J.; Hellinga, H.; Kearney, A.; van der Merwe, P.; Yang, J.. "Design of a Calcium-Binding Protein with Desired Structure in a Cell Adhesion Molecule" J. Am. Chem. Soc. 127, 2085-2093 (2005).
PubMed: 15713084

Assembly members:

Assembly members:
Ca.CD2, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ca.CD2: RDSGTVWGALGHGIDLDIPN FQMTDDIDEVRWERGSTLVA EFKRKMKPFLKSGAFEILAN GDLKIKNLTRDDSGTYNVTV YSTNGTRILNKALDLRILE

Data sets:

assigned_chemical_shifts
- shift_1
- shift_2

Data type	Count
15N chemical shifts	202
1H chemical shifts	1072

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD2 with designed calcium binding site	1

Entities:

Entity 1, CD2 with designed calcium binding site 99 residues - Formula weight is not available

1

ARG

ASP

SER

GLY

THR

VAL

TRP

GLY

ALA

LEU

2

GLY

HIS

GLY

ILE

ASP

LEU

ASP

ILE

PRO

ASN

3

PHE

GLN

MET

THR

ASP

ILE

ASP

GLU

VAL

4

ARG

TRP

GLU

ARG

GLY

SER

THR

LEU

VAL

ALA

5

GLU

PHE

LYS

ARG

LYS

MET

LYS

PRO

PHE

LEU

6

LYS

SER

GLY

ALA

PHE

GLU

ILE

LEU

ALA

ASN

7

GLY

ASP

LEU

LYS

ILE

LYS

ASN

LEU

THR

ARG

8

ASP

SER

GLY

THR

TYR

ASN

VAL

THR

VAL

9

TYR

SER

THR

ASN

GLY

THR

ARG

ILE

LEU

ASN

10

LYS

ALA

LEU

ASP

LEU

ARG

ILE

LEU

GLU

Samples:

sample_1: Ca.CD2, [U-95% 15N], 1.0 mM; KCl 130 mM; CaCl2 10 mM; PIPES 20 mM

sample_2: Ca.CD2, [U-95% 15N], 1.0 mM; KCl 130 mM; EGTA 3 mM; PIPES 20 mM

Conditions_1: pH: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	Conditions_1

Software:

SPARKY -

NMR spectrometers:

unknown unknown 0 MHz

BMRB	4109
PDB	1CDC 1HNG 1T6W
EMBL	CAA28757
GB	AAH88164 ADI96088 EDL85525
REF	NP_036962
SP	P08921
AlphaFold	P08921