BMRB Entry 5799

Name: Protein Signal Assignments using Specific Labeling and Cell-free Synthesis
Published: 2004-03-23

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5799

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Protein Signal Assignments using Specific Labeling and Cell-free Synthesis

Deposition date:

2003-05-16

Original release date:

2004-03-23

Authors:

Shi, Jianxia; Pelton, Jefferey

Citation:

Citation: Shi, Jianxia; Pelton, Jefferey; Cho, H.; Wemmer, D.. "Protein Signal Assignments using Specific Labeling and Cell-free Synthesis" J. Biomol. NMR 28, 235-247 (2004).
PubMed: 14752257

Assembly members:

Assembly members:
Phosphoserine phosphatase, polymer, 211 residues, 23594 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 2190 Superkingdom: Eubacteria Kingdom: not available Genus/species: Methanococcous jannichi

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Phosphoserine phosphatase: MEKKKKLILFDFDSTLVNNE TIDEIAREAGVEEEVKKITK EAMEGKLNFEQSLRKRVSLL KDLPIEKVEKAIKRITPTEG AEETIKELKNRGYVVAVVSG GFDIAVNKIKEKLGLDYAFA NRLIVKDGKLTGDVEGEVLK ENAKGEILEKIAKIEGINLE DTVAVGDGANDISMFKKAGL KIAFCAKPILKEKADICIEK RDLREILKYIK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	557
13C chemical shifts	599
15N chemical shifts	206

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PSP monomer	1

Entities:

Entity 1, PSP monomer 211 residues - 23594 Da.

1

MET

GLU

LYS

LEU

ILE

LEU

PHE

2

ASP

PHE

ASP

SER

THR

LEU

VAL

ASN

GLU

3

THR

ILE

ASP

GLU

ILE

ALA

ARG

GLU

ALA

GLY

4

VAL

GLU

VAL

LYS

ILE

THR

LYS

5

GLU

ALA

MET

GLU

GLY

LYS

LEU

ASN

PHE

GLU

6

GLN

SER

LEU

ARG

LYS

ARG

VAL

SER

LEU

7

LYS

ASP

LEU

PRO

ILE

GLU

LYS

VAL

GLU

LYS

8

ALA

ILE

LYS

ARG

ILE

THR

PRO

THR

GLU

GLY

9

ALA

GLU

THR

ILE

LYS

GLU

LEU

LYS

ASN

10

ARG

GLY

TYR

VAL

ALA

VAL

SER

GLY

11

GLY

PHE

ASP

ILE

ALA

VAL

ASN

LYS

ILE

LYS

12

GLU

LYS

LEU

GLY

LEU

ASP

TYR

ALA

PHE

ALA

13

ASN

ARG

LEU

ILE

VAL

LYS

ASP

GLY

LYS

LEU

14

THR

GLY

ASP

VAL

GLU

GLY

GLU

VAL

LEU

LYS

15

GLU

ASN

ALA

LYS

GLY

GLU

ILE

LEU

GLU

LYS

16

ILE

ALA

LYS

ILE

GLU

GLY

ILE

ASN

LEU

GLU

17

ASP

THR

VAL

ALA

VAL

GLY

ASP

GLY

ALA

ASN

18

ASP

ILE

SER

MET

PHE

LYS

ALA

GLY

LEU

19

LYS

ILE

ALA

PHE

CYS

ALA

LYS

PRO

ILE

LEU

20

LYS

GLU

LYS

ALA

ASP

ILE

CYS

ILE

GLU

LYS

21

ARG

ASP

LEU

ARG

GLU

ILE

LEU

LYS

TYR

ILE

22

LYS

Samples:

Sample1: Phosphoserine phosphatase 1.0 mM

Ex-cond_1: pH: 6.2 na; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N hsqc	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
HNCO	not available	not available	not available
HNcaCO	not available	not available	not available
HNcoCA	not available	not available	not available
CBCAcoNH	not available	not available	not available
HAHN	not available	not available	not available
HBHAcoNH	not available	not available	not available
HNcaNH	not available	not available	not available
15N NOESY	not available	not available	not available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1F5S 1J97 1L7M 1L7N 1L7O 1L7P
GB	AAB99612 AIJ05608
REF	NP_248603 WP_010871118
SP	Q58989
AlphaFold	Q58989