BMRB Entry 5659

Name: 1H, 13C and 15N Backbone Assignments for the Human Rhinovirus-14 3C Protease
Published: 2006-07-21

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PDB ID: 2in2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5659
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Backbone Assignments for the Human Rhinovirus-14 3C Protease

Deposition date:

2003-01-13

Original release date:

2006-07-21

Authors:

Bjorndahl, Trent; Monzavi, Hassan; Wishart, David

Citation:

Citation: Bjorndahl, Trent; Monzavi, Hassan; Wishart, David. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the human rhinovirus 3C protease (serotype 14) " J. Biomol. NMR 26, 85-86 (2003).

Assembly members:

Assembly members:
HRV14-3C, polymer, 182 residues, 19996.90 Da.

Natural source:

Natural source: Common Name: Human Rhinovirus-14 Taxonomy ID: 12131 Superkingdom: Virus Kingdom: Picornaviridae Genus/species: Rhinovirus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HRV14-3C: GPNTEFALSLLRKNIMTITT SKGEFTGLGIHDRVCVIPTH AQPGDDVLVNGQKIRVKDKY KLVDPENINLELTVLTLDRN EKFRDIRGFISEDLEGVDAT LVVHSNNFTNTILEVGPVTM AGLINLSSTPTNRMIRYDYA TKTGQCGGVLCATGKIFGIH VGGNGRQGFSAQLKKQYFVE KQ

Data sets:

assigned_chemical_shifts
- chemical_shifts

Data type	Count
1H chemical shifts	1163
13C chemical shifts	683
15N chemical shifts	159

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human Rhinovirus-14 3C Protease	1

Entities:

Entity 1, Human Rhinovirus-14 3C Protease 182 residues - 19996.90 Da.

1

GLY

PRO

ASN

THR

GLU

PHE

ALA

LEU

SER

LEU

2

LEU

ARG

LYS

ASN

ILE

MET

THR

ILE

THR

3

SER

LYS

GLY

GLU

PHE

THR

GLY

LEU

GLY

ILE

4

HIS

ASP

ARG

VAL

CYS

VAL

ILE

PRO

THR

HIS

5

ALA

GLN

PRO

GLY

ASP

VAL

LEU

VAL

ASN

6

GLY

GLN

LYS

ILE

ARG

VAL

LYS

ASP

LYS

TYR

7

LYS

LEU

VAL

ASP

PRO

GLU

ASN

ILE

ASN

LEU

8

GLU

LEU

THR

VAL

LEU

THR

LEU

ASP

ARG

ASN

9

GLU

LYS

PHE

ARG

ASP

ILE

ARG

GLY

PHE

ILE

10

SER

GLU

ASP

LEU

GLU

GLY

VAL

ASP

ALA

THR

11

LEU

VAL

HIS

SER

ASN

PHE

THR

ASN

12

THR

ILE

LEU

GLU

VAL

GLY

PRO

VAL

THR

MET

13

ALA

GLY

LEU

ILE

ASN

LEU

SER

THR

PRO

14

THR

ASN

ARG

MET

ILE

ARG

TYR

ASP

TYR

ALA

15

THR

LYS

THR

GLY

GLN

CYS

GLY

VAL

LEU

16

CYS

ALA

THR

GLY

LYS

ILE

PHE

GLY

ILE

HIS

17

VAL

GLY

ASN

GLY

ARG

GLN

GLY

PHE

SER

18

ALA

GLN

LEU

LYS

GLN

TYR

PHE

VAL

GLU

19

LYS

GLN

Samples:

sample_1: HRV14-3C, [U-15N], 1 mM; KH2PO4 20 mM; NaCl 0 mM

sample_2: HRV14-3C, [U-15N; U-13C], 1 mM; KH2PO4 20 mM; NaCl 0 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCO	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONNH	not available	not available	not available
HNHA	not available	not available	not available
15N-HSQC	not available	not available	not available

Software:

NMRPIPE - specta processing

NMRVIEW - peak picking, spectral analysis

NMR spectrometers:

Varian Unity 500 MHz

PDB	2B0F 2IN2
EMBL	CAA25565
GB	AAA45756 AAA45757 AAA45758
REF	NP_041009 NP_740524
SP	P03303
AlphaFold	P03303