BMRB Entry 5552

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PDB ID: 1m3g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5552
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments of the Catalytic Domain of Pac1

Deposition date:

2002-10-10

Original release date:

2003-03-14

Authors:

Farooq, A.; Zhou, M.-M.

Citation:

Citation: Farooq, A.; Plotnikova, O.; Chaturvedi, G.; Yan, S.; Zeng, L.; Zhang, Q.; Zhou, M.-M.. "Solution Structure of the MAPK Phosphotase PAC-1: Catalytic Domain. Insights into Substrate-induced Enzymatic Activation of MKP" Structure 11, 155-164 (2003).
PubMed: 12575935

Assembly members:

Assembly members:
dual specificity protein phosphatase 2, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dual specificity protein phosphatase 2: QGGPVEILPYLFLGSCSHSS DLQGLQACGITAVLNVSASC PNHFEGLFRYKSIPVEDNQM VEISAWFQEAIGFIDWVKNS GGRVLVHSQAGISRSATICL AYLMQSRRVRLDEAFDFVKQ RRGVISPNFSFMGQLLQFET QVLCH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	666
13C chemical shifts	334
15N chemical shifts	124

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dual specificity protein phosphatase 2	1

Entities:

Entity 1, dual specificity protein phosphatase 2 145 residues - Formula weight is not available

1

GLN

GLY

PRO

VAL

GLU

ILE

LEU

PRO

TYR

2

LEU

PHE

LEU

GLY

SER

CYS

SER

HIS

SER

3

ASP

LEU

GLN

GLY

LEU

GLN

ALA

CYS

GLY

ILE

4

THR

ALA

VAL

LEU

ASN

VAL

SER

ALA

SER

CYS

5

PRO

ASN

HIS

PHE

GLU

GLY

LEU

PHE

ARG

TYR

6

LYS

SER

ILE

PRO

VAL

GLU

ASP

ASN

GLN

MET

7

VAL

GLU

ILE

SER

ALA

TRP

PHE

GLN

GLU

ALA

8

ILE

GLY

PHE

ILE

ASP

TRP

VAL

LYS

ASN

SER

9

GLY

ARG

VAL

LEU

VAL

HIS

SER

GLN

ALA

10

GLY

ILE

SER

ARG

SER

ALA

THR

ILE

CYS

LEU

11

ALA

TYR

LEU

MET

GLN

SER

ARG

VAL

ARG

12

LEU

ASP

GLU

ALA

PHE

ASP

PHE

VAL

LYS

GLN

13

ARG

GLY

VAL

ILE

SER

PRO

ASN

PHE

SER

14

PHE

MET

GLY

GLN

LEU

GLN

PHE

GLU

THR

15

GLN

VAL

LEU

CYS

HIS

Samples:

sample_1: dual specificity protein phosphatase 2, [U-13C; U-15N], 0.5 mM; phosphate buffer 5 mM

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available

Software:

XPLOR - structure solution, refinement

ARIA - structure solution, refinement

XWINNMR - collection

NMRPIPE - processing

NMRVIEW - data analysis

NMR spectrometers:

BRUKER DRX 600 MHz

BMRB	5000
PDB	1M3G
GB	AAA50779 AAA86112 AAH07771 AAL57044 AAY24222
REF	NP_004409 XP_001111118 XP_002757414 XP_002811694 XP_003281084
SP	Q05923
AlphaFold	Q05923