BMRB Entry 53200

Name: drp18
Published: 2025-06-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53200

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

drp18

Deposition date:

2025-05-22

Original release date:

2025-06-13

Authors:

Sethi, Aakriti; de Cordovez, Pierre; Mohanty, Biswaranjan; Morris, Vanessa; Gobl, Christoph

Citation:

Citation: Sethi, Aakriti; de Cordovez, Pierre; Mohanty, Biswaranjan; Morris, Vanessa; Gobl, Christoph. "1H, 13C, 15N backbone chemical shift assignment of p18ink4c from Danio rerio (Zebrafish) using solution-state NMR" Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 172 residues, Formula weight is not available

Natural source:

Natural source: Common Name: zebrafish Taxonomy ID: 7955 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Danio rerio

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETZ2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMAEDTAIDRLSTAAAIGD LMEVEQTLQSNVNVNEKNKY GRTALQVMKLGCPSIAETLL QAGADPNVRDPILGLTVIHD AARDGYLDTLHVLAQNGADV NLLDNDGNLPLHLAAREGHL DVVQFLVTHCVTQPFLANAK GYTPRDLAFMHQKHRTVEWL ESIASLQSSQRL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	441
15N chemical shifts	156
1H chemical shifts	519

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	drp18	1

Entities:

Entity 1, drp18 172 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

GLU

ASP

THR

ALA

ILE

ASP

2

ARG

LEU

SER

THR

ALA

ILE

GLY

ASP

3

LEU

MET

GLU

VAL

GLU

GLN

THR

LEU

GLN

SER

4

ASN

VAL

ASN

VAL

ASN

GLU

LYS

ASN

LYS

TYR

5

GLY

ARG

THR

ALA

LEU

GLN

VAL

MET

LYS

LEU

6

GLY

CYS

PRO

SER

ILE

ALA

GLU

THR

LEU

7

GLN

ALA

GLY

ALA

ASP

PRO

ASN

VAL

ARG

ASP

8

PRO

ILE

LEU

GLY

LEU

THR

VAL

ILE

HIS

ASP

9

ALA

ARG

ASP

GLY

TYR

LEU

ASP

THR

LEU

10

HIS

VAL

LEU

ALA

GLN

ASN

GLY

ALA

ASP

VAL

11

ASN

LEU

ASP

ASN

ASP

GLY

ASN

LEU

PRO

12

LEU

HIS

LEU

ALA

ARG

GLU

GLY

HIS

LEU

13

ASP

VAL

GLN

PHE

LEU

VAL

THR

HIS

CYS

14

VAL

THR

GLN

PRO

PHE

LEU

ALA

ASN

ALA

LYS

15

GLY

TYR

THR

PRO

ARG

ASP

LEU

ALA

PHE

MET

16

HIS

GLN

LYS

HIS

ARG

THR

VAL

GLU

TRP

LEU

17

GLU

SER

ILE

ALA

SER

LEU

GLN

SER

GLN

18

ARG

LEU

Samples:

sample_1: drp18, [U-99% 13C; U-99% 15N], 200 uM; Phosphate buffer 50 mM; DTT (Dithiothreitol) 2 mM; EDTA 200 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v11.7 - processing

CcpNMR v3.2.0 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 800 MHz