BMRB Entry 53082

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53082

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Title:
Slf1 La motif domain from Saccharomyces cerevisiae
Deposition date:
2025-04-28
Original release date:
2025-05-23
Authors:
Donaldson, Logan
Citation:

Citation: Pacheco, Evan; Shoara, Aron; Donaldson, Logan. "RNA binding by the yeast Slf1 and Sro9 LaM domains"  .

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKSIESIKNQIEFYFSEENL KTDEFLRSKFKKANDGFIPM SLIGKFYRMVNLSLGGDPNL ILASMREVLQHKETNHLEIA LGSIEGAQKNMADDFNPLEN YFIRRENWAEYAMESNFDEN DDETEKYNIEKLLGPNDLDN YSYLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts423
15N chemical shifts109
1H chemical shifts532

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Slf1 La motif domain1

Entities:

Entity 1, Slf1 La motif domain 151 residues - Formula weight is not available

LE + HHHHHH is added to the C-terminus of the native sequence from cloning

1   METLYSSERILEGLUSERILELYSASNGLN
2   ILEGLUPHETYRPHESERGLUGLUASNLEU
3   LYSTHRASPGLUPHELEUARGSERLYSPHE
4   LYSLYSALAASNASPGLYPHEILEPROMET
5   SERLEUILEGLYLYSPHETYRARGMETVAL
6   ASNLEUSERLEUGLYGLYASPPROASNLEU
7   ILELEUALASERMETARGGLUVALLEUGLN
8   HISLYSGLUTHRASNHISLEUGLUILEALA
9   LEUGLYSERILEGLUGLYALAGLNLYSASN
10   METALAASPASPPHEASNPROLEUGLUASN
11   TYRPHEILEARGARGGLUASNTRPALAGLU
12   TYRALAMETGLUSERASNPHEASPGLUASN
13   ASPASPGLUTHRGLULYSTYRASNILEGLU
14   LYSLEULEUGLYPROASNASPLEUASPASN
15   TYRSERTYRLEUGLUHISHISHISHISHIS
16   HIS

Samples:

sample_1: protein, [U-99% 13C; U-99% 15N], 0.4 mM; sodium chloride 50 mM; sodium phosphate 10 mM; sodium azide 0.05 % w/v

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.52 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks