BMRB Entry 52956

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52956

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Title:
Assembly and functional mechanisms of the Hsp70-Hsp40 chaperone machinery
Deposition date:
2025-03-09
Original release date:
2025-10-20
Authors:
Jiang, Yajun; Ibrahim, Ziad; Clay, Mary; Xia, Youlin; Kalodimos, Babis
Citation:

Citation: Jiang, Yajun; Ibrahim, Ziad; Xia, Youlin; Clay, Mary; Myasnikov, Alexander; Immadisetty, Kalyan; Xia, Zhilian; Tang, Liang; Rossi, Paolo; Ganguly, Pritha; Liu, Jiangshu; Miller, Darcie; Che, Meixia; Palacios, Santiago; Kramer, Gunter; Bukau, Bernd; Kalodimos, Charalampos. "Mechanisms of assembly and function of the Hsp70-Hsp40 chaperone machinery"  Mol. Cell ., .-. (2025).
PubMed: 41092901

Assembly members:

Assembly members:
entity_1, polymer, 382 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Thermus thermophilus   Vector: pCoofy

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAKAVGIDLGTTNSVIAVLE GGKPVVLENAEGERVTPSVV AFRDGETLVGRMAKRQAVLN PEGTIFEIKRFIGRRFEEVQ EEAKRVPYKVVPGPDGGVRV EVKGKLYTPEEISAMILRKL VEDASKKLGEKITKAVITVP AYFNNAQREATANAGRIAGL EVLRIINEPTAAALAYGLDK KGNETVLVFDLGGGTFDVTI LEIGEGVFEVKATSGDTHLG GSDMDHAIVNWLAEEFKKEH GVDLKADRQALQRLIEAAEK AKIELSSTLETTISLPFIAL DPASKTPLHLEKKLTRAKFE ELIQPLLKRLRGPVEQALKD AGLTPAQIDEVILVGGATRV PAVQQVVRELLGKEPNRSVN PDEVVAMGAAIQAGVLMGEV RD

Data sets:
Data typeCount
15N chemical shifts357
1H chemical shifts357

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BaKNBD1

Entities:

Entity 1, BaKNBD 382 residues - Formula weight is not available

1   METALALYSALAVALGLYILEASPLEUGLY
2   THRTHRASNSERVALILEALAVALLEUGLU
3   GLYGLYLYSPROVALVALLEUGLUASNALA
4   GLUGLYGLUARGVALTHRPROSERVALVAL
5   ALAPHEARGASPGLYGLUTHRLEUVALGLY
6   ARGMETALALYSARGGLNALAVALLEUASN
7   PROGLUGLYTHRILEPHEGLUILELYSARG
8   PHEILEGLYARGARGPHEGLUGLUVALGLN
9   GLUGLUALALYSARGVALPROTYRLYSVAL
10   VALPROGLYPROASPGLYGLYVALARGVAL
11   GLUVALLYSGLYLYSLEUTYRTHRPROGLU
12   GLUILESERALAMETILELEUARGLYSLEU
13   VALGLUASPALASERLYSLYSLEUGLYGLU
14   LYSILETHRLYSALAVALILETHRVALPRO
15   ALATYRPHEASNASNALAGLNARGGLUALA
16   THRALAASNALAGLYARGILEALAGLYLEU
17   GLUVALLEUARGILEILEASNGLUPROTHR
18   ALAALAALALEUALATYRGLYLEUASPLYS
19   LYSGLYASNGLUTHRVALLEUVALPHEASP
20   LEUGLYGLYGLYTHRPHEASPVALTHRILE
21   LEUGLUILEGLYGLUGLYVALPHEGLUVAL
22   LYSALATHRSERGLYASPTHRHISLEUGLY
23   GLYSERASPMETASPHISALAILEVALASN
24   TRPLEUALAGLUGLUPHELYSLYSGLUHIS
25   GLYVALASPLEULYSALAASPARGGLNALA
26   LEUGLNARGLEUILEGLUALAALAGLULYS
27   ALALYSILEGLULEUSERSERTHRLEUGLU
28   THRTHRILESERLEUPROPHEILEALALEU
29   ASPPROALASERLYSTHRPROLEUHISLEU
30   GLULYSLYSLEUTHRARGALALYSPHEGLU
31   GLULEUILEGLNPROLEULEULYSARGLEU
32   ARGGLYPROVALGLUGLNALALEULYSASP
33   ALAGLYLEUTHRPROALAGLNILEASPGLU
34   VALILELEUVALGLYGLYALATHRARGVAL
35   PROALAVALGLNGLNVALVALARGGLULEU
36   LEUGLYLYSGLUPROASNARGSERVALASN
37   PROASPGLUVALVALALAMETGLYALAALA
38   ILEGLNALAGLYVALLEUMETGLYGLUVAL
39   ARGASP

Samples:

sample_1: DnaKNBD, [U-100% 13C; U-100% 15N; U-100% 2H], 350 uM; H2O 95%; D2O, [U-100% 2H], 5%; potassium phosphate 25 mM; sodium chloride 75 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks