BMRB Entry 52884

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52884

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Title:
Backbone resonance assignments of the human Bcl-2 in micelles
Deposition date:
2025-01-23
Original release date:
2025-07-03
Authors:
Nagy, Tamas Milan; Mushtaq, Ameeq Ul; Aden, Jorgen; Grobner, Gerhard
Citation:

Citation: Nagy, Tamas Milan; Mushtaq, Ameeq Ul; Aden, Jorgen; Williams, Chloe; Ayscough, Sophie; Gilthorpe, Jonathan; E. Clifton, Luke; Grobner, Gerhard. "The Human Bcl-2 Interacts with Apo hSOD1 and Its Disease-Related mutants"  .

Assembly members:

Assembly members:
entity_1, polymer, 239 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAHAGRTGYDNREIVMKYIH YKLSQRGYEWDAGDVGAAPP GAAPAPGIFSSQPGHTPHPA ASRDPVARTSPLQTPAAPGA AAGPALSPVPPVVHLTLRQA GDDFSRRYRRDFAEMSSQLH LTPFTARGRFATVVEELFRD GVNWGRIVAFFEFGGVMCVE SVNREMSPLVDNIALWMTEY LNRHLHTWIQDNGGWDAFVE LYGPSMRPLFDFSWLSLKTL LSLALVGACITLGAYLGHK

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts132
1H chemical shifts188

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bcl-21

Entities:

Entity 1, Bcl-2 239 residues - Formula weight is not available

1   METALAHISALAGLYARGTHRGLYTYRASP
2   ASNARGGLUILEVALMETLYSTYRILEHIS
3   TYRLYSLEUSERGLNARGGLYTYRGLUTRP
4   ASPALAGLYASPVALGLYALAALAPROPRO
5   GLYALAALAPROALAPROGLYILEPHESER
6   SERGLNPROGLYHISTHRPROHISPROALA
7   ALASERARGASPPROVALALAARGTHRSER
8   PROLEUGLNTHRPROALAALAPROGLYALA
9   ALAALAGLYPROALALEUSERPROVALPRO
10   PROVALVALHISLEUTHRLEUARGGLNALA
11   GLYASPASPPHESERARGARGTYRARGARG
12   ASPPHEALAGLUMETSERSERGLNLEUHIS
13   LEUTHRPROPHETHRALAARGGLYARGPHE
14   ALATHRVALVALGLUGLULEUPHEARGASP
15   GLYVALASNTRPGLYARGILEVALALAPHE
16   PHEGLUPHEGLYGLYVALMETCYSVALGLU
17   SERVALASNARGGLUMETSERPROLEUVAL
18   ASPASNILEALALEUTRPMETTHRGLUTYR
19   LEUASNARGHISLEUHISTHRTRPILEGLN
20   ASPASNGLYGLYTRPASPALAPHEVALGLU
21   LEUTYRGLYPROSERMETARGPROLEUPHE
22   ASPPHESERTRPLEUSERLEULYSTHRLEU
23   LEUSERLEUALALEUVALGLYALACYSILE
24   THRLEUGLYALATYRLEUGLYHISLYS

Samples:

sample_1: Bcl-2, [U-100% 13C; U-100% 15N; U-80% 2H], 0.4 mM; DPC, [U-99% 2H], 5 mM; PBS 20 mM; TCFP 5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6; pressure: 1 atm; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks